Ov 90

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

The OV-90 is a compact and robust vacuum oven designed for a variety of laboratory applications. It offers precise temperature control and uniform heating within its interior chamber. The OV-90 is suitable for tasks such as drying, curing, and heat treatment of samples.

Automatically generated - may contain errors

Lab products found in correlation

12 protocols using ov 90

Ovarian Cancer Cell Line Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ovarian cancer cell lines, OVCAR3, OV-90, PEO1 and PEO4 were purchased from ATCC (American Type Culture Collection) and were maintained, respectively, in RPMI (OVCAR3, PEO1 and PEO4) and DMEM (OV-90) (Gibco, Paisley, UK), supplemented with 20% (OVCAR-3) or 10% (OV-90, PEO1 and PEO4) fetal bovine serum (FBS; Gibco BRL, Italia), 1% L- Glutamine and 1% of penicillin – streptomycin (Gibco, Paisley, UK). Kuramochi cells were purchased from Sekisui XenoTech, LLC and were maintained in RPMI (Gibco, Paisley, UK), supplemented with 10% FBS, 1% L-Glutamine and 1% of penicillin – streptomycin (Gibco, Paisley, UK). Olaparib (AZD2281), and P005091 were provided by SelleckChem. PARG inhibitor (PDD00017273), SB-216763, cycloheximide, MG132 and cisplatin were provided by Merk Millipore. The genetic background of the ovarian cancer cells is reported in Additional File 1: Table S1.

Morra F., Merolla F., Damia G., Ricci F., Varricchio S., Ilardi G., Arenare L., Califano D., Napolitano V., Fruscio R., Melillo R.M., Palazzo L, & Celetti A. (2022). The disruption of the CCDC6 – PP4 axis induces a BRCAness like phenotype and sensitivity to PARP inhibitors in high-grade serous ovarian carcinoma. Journal of Experimental & Clinical Cancer Research : CR, 41, 245.

+ Open protocol

+ Expand

Cisplatin-Resistant Ovarian Cancer Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human high-grade serous ovarian cancer cell lines OV-90 and OVCAR3 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). OV-90CIS and OVCAR3CIS cells were generated by exposing parental cell lines to increasing doses of cisplatin as previously described [70 (link)]. OV-90 and OV-90CIS cells were maintained in M199 (Gibco, Thermo Fisher Scientific, Grand Island, NY, USA)/MCDB-105 (Sigma-Aldrich, St. Louis, MO, USA) media. OVCAR3 and OVCAR3CIS cells were maintained in RPMI-1640 (Thermo Scientific, Grand Island, NY, USA) medium supplemented with 0.01 mg/mL insulin (Sigma-Aldrich). In all cases, the medium was supplemented with 10% fetal bovine serum (FBS; HyClone, GE Healthcare Life Sciences, Logan, UT, USA) and 0.1% antibiotic/antimycotic solution (HyClone). The cells were incubated at 37 °C in 5% CO₂ with 95% air. All cell lines were screened for mycoplasma using the LookOut^® Mycoplasma PCR detection kit (Sigma). In vitro experiments were performed at 70–85% cell confluence.

Flores-Colón M., Rivera-Serrano M., Reyes-Burgos V.G., Rolón J.G., Pérez-Santiago J., Marcos-Martínez M.J., Valiyeva F, & Vivas-Mejía P.E. (2024). MicroRNA Expression Profiles in Human Samples and Cell Lines Revealed Nine miRNAs Associated with Cisplatin Resistance in High-Grade Serous Ovarian Cancer. International Journal of Molecular Sciences, 25(7), 3793.

+ Open protocol

+ Expand

Culturing Ovarian and Fallopian Epithelial Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The SV40 transformed primary normal ovarian epithelial cell line (OSE tsT/hTERT, henceforth OSE) (18 (link)) was a kind gift from Dr. V. Shridhar, Mayo Clinic, Rochester, MN, USA. The SV40 transformed primary normal fallopian tube epithelial cells (henceforth FTE188) (19 (link)) were kindly provided by Dr. Jinsong Liu, MD Anderson Cancer Center, Houston, TX, USA. The CP20 cell line was a kind gift from Dr. Anil K. Sood, MD Anderson Cancer Center, Houston, TX, and was authenticated by the STR profiling facility at MD Anderson Cancer Center. The OV90, and OVCAR4 cell lines were purchased from ATCC and NCI respectively and OVSAHO, TYKNU, KURAMOCHI, OVKATE were purchased from JCRB Cell Bank (Japanese Collection of Research Bioresources Cell Bank). OSE cells were routinely cultured in 1:1 MCDB 105 and Medium 199 (Corning, Corning, NY, USA) + 10% FBS (Gibco, Grand Island, NY); FTE188 cells were cultured in 1:1 MCDB 105 and Medium 199 + 10% FBS + 0.01ug/ml EGF; CP20, OV90 and OVCAR4 were routinely cultured in RPMI + 10% FBS. All the cells were cultured with 1× penicillin-streptomycin (Gibco, Grand Island, NY) in a 5% CO₂ humidified atmosphere and tested for mycoplasma contamination prior to any experiment.

Dwivedi S.K., Khader S., Dey A., Mustafi S.B., Xiong X., Bhattacharya U., Neizer-Ashun F., Rao G., Wang Y., Ivan C., Yang D., Dudley J.T., Xu C., Wren J.D., Mukherjee P, & Bhattacharya R. (2019). KRCC1: a potential therapeutic target in ovarian cancer. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(2), 2287-2300.

+ Open protocol

+ Expand

Cultivation of Human Ovarian Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human serous OC cell line OV-90 and human epithelial OC cell line SKOV-3 were obtained from Korean Biotech Co., Ltd., Seoul, Korea the domestic distributor of American Type Culture Collection (ATCC). The OV-90 was cultured in a 1:1 mixture of MCDB 105 medium (LM016-01) and Medium 199 (#GIB-11150-059, Gibco, Life Technologies, Grand Island, NY, USA), while SKOV-3 was cultured in McCoy’s 5A modified (#GIB-16600082, Gibco). All media are supplemented with 10% fetal bovine serum (FBS, #GIB-16000-044, Gibco) and 1% penicillin-streptomycin (#P4333, Sigma-Aldrich, St. Louis, MO, USA). Cells were grown in 5% CO₂ saturated humidity, at 37 °C, and sub-cultured by harvesting with trypsin-ethylenediaminetetraacetic acid (EDTA) (#GIB-25300-054, Life Technologies, Grand Island, NY, USA).

Bahar E., Kim J.Y., Kim H.S, & Yoon H. (2020). Establishment of Acquired Cisplatin Resistance in Ovarian Cancer Cell Lines Characterized by Enriched Metastatic Properties with Increased Twist Expression. International Journal of Molecular Sciences, 21(20), 7613.

+ Open protocol

+ Expand

Ovarian Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human epithelial ovarian cancer cell lines, namely, OV-90 (MZ-2097, MINGZHOUBIO, Ningbo, China) and A2780 (CBP60283, Cobioer Biosciences, Nanjing, China), were stored in our laboratory. A2780—ovarian endometrioid adenocarcinoma. OV-90—High-grade serous ovarian cancer derived from metastatic site: ascites. Mouse epithelial ovarian cancer cell line ID-8 was stored in our laboratory. ID-8, OV-90, and A2780 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, C11995500BT) in an incubator with 5% CO₂ at 37 °C. The complete medium contained 10% fetal bovine serum (FBS, Biological Industries, Israel, C04001500) and 1% penicillin–streptomycin (Cytiva, Logan, UT, USA, SV30010).

Liu W., Wang L., Zhang J., Cheng K., Zheng W, & Ma Z. (2023). CC Chemokine 2 Promotes Ovarian Cancer Progression through the MEK/ERK/MAP3K19 Signaling Pathway. International Journal of Molecular Sciences, 24(13), 10652.

+ Open protocol

+ Expand

Isolation and Culture of Chemosensitive and Chemoresistant Ovarian Cancer Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chemosensitive (n = 5) and chemoresistant (n = 7) primary cells were obtained from ascites of high grade OC patients before and after chemotherapy treatment as previously described [103 (link)]. Chemoresistance was determined when patients relapse and no longer respond to chemotherapy. Chemosensitivity was classified as patients responding to chemotherapy and not progressing within 6 months of completing their treatment. These primary cells were grown in Advanced RPMI 1640 media (Gibco, Waltham, MA, USA, cat no. 12633-020) containing 10% fetal calf serum (FCS) (Scientifix, Clayton, VIC, Australia) and 1% each of penicillin/streptomycin (Life Technologies, Mulgrave, VIC, Australia), fungizone (Sigma−Aldrich, St. Louis, MO, USA) and glutamax (Life Technologies, Mulgrave, VIC, Australia) and maintained at 37 °C in a 6% CO₂ environment.
Human HGSOC cell lines, OVCAR-3 and OV-90, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). They were cultured in RPMI 1640 (Gibco, Waltham, MA, USA, cat no. 11875-093) with FCS (5% for OVCAR-3, 10% for OV-90), 1% penicillin/streptomycin and 1% glutamax.

Lee E., Lokman N.A., Oehler M.K., Ricciardelli C, & Grutzner F. (2020). A Comprehensive Molecular and Clinical Analysis of the piRNA Pathway Genes in Ovarian Cancer. Cancers, 13(1), 4.

+ Open protocol

+ Expand

Ovarian Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

OC cell lines (A2780, OV90, OVCAR3, HEY) and normal ovarian epithelial cell lines (IOSE-80) were purchased from the American Type Culture Collection. IOSE-80 cells were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.); A2780, OV90, OVCAR3 and HEY cells were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) containing 10% FBS. All cells were cultured in 5% CO₂ incubator at 37˚C.

Chen H., Liu Y., Liu P., Dai Q, & Wang P. (2021). LINC01094 promotes the invasion of ovarian cancer cells and regulates the Wnt/β-catenin signaling pathway by targeting miR-532-3p. Experimental and Therapeutic Medicine, 22(5), 1228.

+ Open protocol

+ Expand

Silencing CaMKK2 in Ovarian Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two OC cell lines (HO8910 and OV90 cells) were obtained from Hunan Fenghui Biotechnology Co., Ltd. (Hunan, China). HO8910 and OV90 cells were cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) containing 10% FBS in an incubator at37°C with 5% CO₂. SiRNA targeting CaMKK2 (si-CaMKK2; 5'-GTGTTTACACAGTAAGATCAAGA-3') or CaMKK2-NC synthesized by Genepharma (Shanghai, China) were transfected into HO8910 and OV90 cells using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).

Chen Z., Sun X., Xia Z., Wang J., Guo N, & Zhang Y. (2022). CaMKK2 Promotes the Progression of Ovarian Carcinoma through the PI3K/PDK1/Akt Axis. Computational and Mathematical Methods in Medicine, 2022, 7187940.

+ Open protocol

+ Expand

Cell Line Authentication and Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

CAOV3 and OV90 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). The HEK293T cell line was obtained from the Chinese Academy of Sciences (Shanghai, China). CAOV3 and OV90 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS), while HEK293T cells were maintained in DMEM supplemented with 10% FBS (all from Gibco, Grand Island, NY, USA). A humidified 37 °C incubator with 5% CO₂ was used to culture all cells. Short tandem repeat (STR) profiling was performed to confirm the cells and to exclude mycoplasma contamination.

Qi G., Ma H., Li Y., Peng J., Chen J, & Kong B. (2021). TTK inhibition increases cisplatin sensitivity in high-grade serous ovarian carcinoma through the mTOR/autophagy pathway. Cell Death & Disease, 12(12), 1135.

+ Open protocol

+ Expand

Establishing AZD1775-resistant ovarian cancer cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

A2780 and primary ovarian cancer cells were cultured in RPMI 1640 medium with 10% FBS. OV90 cells were cultured in a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 (Gibco, Thermo Fisher Scientific) containing a final concentration of 2.2 g/L sodium bicarbonate with 15% FBS. SKOV3 cells were cultured in McCoy’s 5a medium with 10% FBS. ES2 and 293T cells were cultured in DMEM with 10% FBS. SKOV3, OV90, and ES2 cells were purchased from the American Type Culture Collection (ATCC). A2780 was obtained from MilliporeSigma. All cell lines were authenticated by short tandem repeat (STR) profiling and tested for mycoplasma contamination. AZD1775-resistant sublines of A2780, SKOV3, OV90, and ES2 were generated by serial passage of cells in the presence of increasing concentrations of AZD1775 and maintained in the presence of 50 nM AZD1775. Lentivirus production and stable gene overexpression in human cells were described previously (39 (link), 40 (link)).

Li J., Lu J., Xu M., Yang S., Yu T., Zheng C., Huang X., Pan Y., Chen Y., Long J., Zhang C., Huang H., Dai Q., Li B., Wang W., Yao S, & Pan C. (2023). ODF2L acts as a synthetic lethal partner with WEE1 inhibition in epithelial ovarian cancer models. The Journal of Clinical Investigation, 133(2), e161544.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!