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Avance 2

Manufactured by Bruker
Sourced in Germany, United States, Switzerland, France

The Avance II is a high-performance nuclear magnetic resonance (NMR) spectrometer developed by Bruker. It is designed to provide researchers and scientists with advanced analytical capabilities for various applications, including chemical analysis, material science, and structural biology. The Avance II offers a range of features and functionalities to support comprehensive NMR investigations, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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229 protocols using avance 2

1

Characterization of Organic Compounds

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Melting points were determined
by an open capillary using a Veego precision digital melting point
apparatus (MP-D) and are uncorrected. 1H NMR spectra were
recorded in CDCl3 with a Bruker AVANCE II (500 MHz) spectrometer
using tetramethylsilane (TMS) as the internal standard. Chemical shift
values are expressed as parts per million downfield from TMS, and
the J values are in hertz. Splitting patterns are
indicated as s: singlet, d: doublet, t: triplet, m: multiplet, dd:
double doublet, ddd: doublet of a doublet of a doublet, and br: broad
peak. 13C NMR spectra were recorded in CDCl3 with a Bruker AVANCE II (125 MHz) spectrometer using TMS as the
internal standard. Mass spectra were recorded on a Bruker high resolution
mass spectrometer (micrOTOF-QII). Elemental analyses were performed
on a Heraeus CHN-O-Rapid elemental analyzer.20e Column chromatography
was performed on a silica gel (60–120 mesh) using an ethyl
chloroform/hexane mixture as the eluent.
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2

NMR Characterization of Organic Compounds

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Proton nuclear magnetic resonance (1H-NMR) spectra were recorded with a Bruker Avance II (Bruker, Rheinstetten, Germany) operating at 600 MHz, with 64 scans, 2.65 s acquisition time, and an 11 µs pulse width. 13C-NMR spectra were recorded with a Bruker Avance II operating at 150.9 MHz, with 20,480 scans, 0.9088 s acquisition times, and 9.40 µs pulse width. The 1H-NMR and 13C-NMR spectra were run in CDCl3 at room temperature with tetramethylsilane (TMS) as an internal standard.
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3

NMR Spectroscopy Protocol for Chemical Analysis

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1H-NMR spectra were recorded with a Bruker Avance II (Bruker, Rheinstetten, Germany) operating at 600 MHz, with 64 scans, 2.65 s acquisition time and 11 µs pulse width. 13C-NMR spectra were recorded with a Bruker Avance II operating at 150.9 MHz, with 20,480 scans, 0.9088 s acquisition times, and 9.40 µs pulse width. 1H-NMR and 13C-NMR spectra were run in CDCl3 at room temperature with tetramethylsilane (TMS) as an internal standard.
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4

Spectroscopic Characterization of BCF and BBF

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The starting chemicals and synthesized BCF and BBF were identified by FT-IR (Fourier transform-infrared) and NMR (nuclear magnetic resonance) spectroscopy. The proton NMR spectra were acquired on a 500 MHz Bruker NMR spectrometer (Bruker Avance II, Bruker BioSpin Corporation, Billerica, MA) with the sample dissolving in deuterated dimethyl sulfoxide. FT-IR spectra were acquired on a FT-IR spectrometer (Mattson Research Series FT/IR 1000, Madison, WI).
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5

Spectroscopic Characterization of Metal Complexes

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Infrared spectra were recorded in the wavenumber region 4000–650 cm−1 on a Spectrum 100 FTIR spectrometer (Perkin-Elmer Inc. MA, USA) equipped with the attenuated total reflectance (ATR) sampling device. 1H, 13C and 31P NMR spectra were recorded on a 300 MHz Bruker AVANCE II and 500 MHz Bruker AVANCE II spectrometer (Bruker Avance Biospin Germany) at the Department of Chemistry of the University of the Witwatersrand (Johannesburg, South Africa). All signals were confirmed by the 1H-1H COSY and 1H-13C HSQC experiments. A temperature-modulated differential scanning calorimeter (Mettler Toledo DSC1 STARe System, Switzerland) was used to investigate the thermal behaviour of the metal complexes. The thermogravimetric (TG and DTG) analyses were performed under a nitrogen atmosphere with a heating rate of 10 °C.min−1 using the Thermogravimetric Analyzer TGA 4000 (Perkin-Elmer Inc. MA, USA). The UV-Vis measurements were recorded on a Lambda 25 UV/VIS Spectrophotometer (Perkin-Elmer Inc. MA, USA). The fluorescence spectrum was recorded on a Perkin Elmer LS-40 fluorescence spectrophotometer (Perkin-Elmer Inc. MA, USA).
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6

Solid-state NMR Spectra Acquisition Protocol

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Solid-state NMR spectra were recorded on a Bruker
Avance II spectrometer (Bruker Biospin, Faellanden, CH) operating
at 700 MHz 1H Larmor frequency (16.4 T), corresponding
to 146 MHz 13C Larmor frequency. The spectrometer is equipped
with a 3.2 BVT MAS probehead in double-resonance mode. The spinning
rate was regulated to 11111 ± 2 Hz using dry air, and the temperature
was set to 280 K. The pulse lengths were 2.5 and 3.5 μs for 1H and 13C, respectively. Cross-polarization20 (link) was achieved by matching the k = 1 Hartmann–Hahn condition.21 (link) For the {1H}13C HETCOR spectra, the spectral
windows for the different nuclei were 60 and 315 ppm for 1H and 13C, respectively. During the 1H magnetization
evolution under the chemical shift in the indirect dimension of heteronuclear
correlation experiments, a PMLG decoupling sequence was used to suppress 1H–1H dipolar couplings.22 (link),23 (link) The CP spectrum was denoised through the MCR procedure.24 (link),25 (link)
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7

Trehalose-modified Hydrogel Characterization

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The trehalose-modified hydrogels were characterized by 1H-NMR (400 MHz Bruker Avance II NMR spectrometer, Bruker BioSpin Co., Switzerland) with deuterated dimethyl sulfoxide as the solvent. NMR peak of the solvent (δ = 2.50 ppm) was used as the reference.
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8

High-Field MRI Imaging Protocol

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All MRI examinations were performed on an 11.7T vertical-bore Bruker Avance II imaging system (Bruker BioSpin, Ettlingen, Germany) with a volume radiofrequency coil for transmission and reception (m2m Imaging Corp., Cleveland, OH, USA).
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9

Characterization of Alumina Particles

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The alumina particle surfaces were characterized with Fourier transform-infrared (FT-IR) and thermal gravity analysis (TGA). FT-IR spectra were acquired on a FT-IR spectrometer (Mattson Research Series FT/IR 1000, Madison, WI). The thermal decomposition history of the selected alumina particles was determined on a thermogravimetric analyzer (Mettler Toledo, Columbus, OH) at a heating rate of 10°C/min under nitrogen. The synthesized derivatives and polymer were characterized by nuclear magnetic resonance (NMR) spectroscopy. The proton NMR (1HNMR) spectra were obtained on a 500 MHz Bruker NMR spectrometer (Bruker Avance II, Bruker Bio-Spin Corporation, Billerica, MA) using deuterated dimethyl sulfoxide as solvent.
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10

Ethyl-AB Identification via NMR Spectroscopy

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Purified ethyl-AB was dried at 30 °C in a centrifugal vacuum concentrator and dissolved in deuterium oxide (D2O), and then it was subjected to nuclear magnetic resonance (NMR) analysis for the identification of ethyl-AB. 1H–13C NMR spectroscopy was performed using a 900 MHz NMR spectrometer equipped with a cryoprobe (Bruker Avance II; Bruker, Billerica, MA, USA). 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid (TSP) was used as a chemical shift reference.
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