Plaque formation assay was used to enumerate the coronavirus. RD cells were seeded in a 12-well plate one day prior to infection. The media containing coronavirus were diluted 10-fold in MEM (Welgene, Seoul, Korea) with 2 % Fetal Bovine Serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA) and 1 % penicillin and streptomycin solution (Welgene). The diluted media were added to RD cells and incubated for 1 h at 33 °C and 5 % CO2. Overlay medium containing 0.6 % agarose was added to each well and incubated for 3 days. After incubation, the cells were fixed with a 10 % formaldehyde solution and stained with 1 % crystal violet to visualize plaques. RD cells were obtained from Korean Cell Line Bank (KCLB, Seoul, Korea) and HCoV-OC43 was obtained from ATCC (Rockville, MD, USA).
Penicillin and streptomycin solution
Manufactured by Welgene
Sourced in Switzerland
Penicillin and streptomycin solution is a sterile liquid media used to supplement cell culture media. It contains the antibiotics penicillin and streptomycin, which inhibit the growth of a wide range of bacteria.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Lonza (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Welgene (1 mentions)
Hcov oc43, by American Type Culture Collection (1 mentions)
Mccoy s 5a medium, by Welgene (1 mentions)
Dulbecco s modified eagle s medium, by Welgene (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Ckx53 microscope, by Olympus (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
4 protocols using penicillin and streptomycin solution
1
Quantifying Coronavirus Plaque Formation
2
Cell culture of HCT116, MCF7, and HEK293T
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MCF7 cells and HEK293T cells were grown in Dulbecco's modified Eagle's medium (WelGENE, Daegu, Korea). DICER knockout (−/−) HCT116 cell line, which was developed by Kim et al. (38 (link)), was purchased from the Korean Collection for Type Cultures (KCTC), Korea Research institute of Bioscience and Biotechnology (KRIBB), Korea. HCT116 parent cells and DICER knockout (−/−) HCT116 cell lines were grown in McCoy's 5A medium (WelGENE). Cells were cultured with 10% Fetal Bovine Serum (Lonza Group Ltd., Basel, Switzerland) and 1% penicillin and streptomycin solution (WelGENE) at 37°C in a 5% CO2 incubator.
3
Metformin-induced Myotube Differentiation
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C2C12 myoblasts were placed in DMEM containing 2% horse serum (Gibco) and 1% penicillin and streptomycin solution (WELGENE) to induce differentiation. After differentiation, Lipofectamine (5
μL
4
Cell Culture of MCF7 and A549
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MCF7 and A549 cells were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and grown in Dulbecco’s modified Eagle’s medium (PAA Laboratories GmbH, Pasching, Austria) and RPMI 1640 (WelGENE, Daegu, Korea), respectively, containing 10% FBS (Lonza Group Ltd., Basel, Switzerland) and 1% penicillin and streptomycin solution (WelGENE) at 37 °C under 5% CO2.
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