B6 129s gt rosa 26sortm1 cag cox8a dendra2 dcc j

PhAM-floxed mice (B6;129S-Gt[ROSA]26Sor^{tm1[CAG-COX8A/Dendra2]Dcc}/J) and VE-Cadherin–Cre mice (B6;129-Tg[Cdh5-cre]1Spe/J) were purchased from The Jackson Laboratory. EC-PhAM mice was generated following the method presented in a previous study (44 (link)). All mice were fed a chow diet and water ad libitum under a 12-hour light/dark cycle. Mice were anesthetized with isoflurane and euthanized by cervical dislocation. For canine aorta samples, animals were euthanized via Euthasol (390 mg/mL pentobarbital sodium and 50 mg/mL phenytoin sodium) at 1 mL/10 lb, i.v. injection. Freshly isolated canine aortas were obtained from one-year-old male dogs (n = 3). Detailed methods for canine aorta collection and EC isolation procedure are provided in Supplemental Methods.

C57BL/6 J mice were purchased Shanghai Jihui Laboratory Animal Care (China); Sod2^+/− mice (B6/JGpt-Sod2^em18Cd19076/Gpt) were purchased from GemPharmatech Company; ROSA26-iDTR^KI mice (C57BL/6-Gt(ROSA)26Sor^{tm1(HBEGF)Awai}/J) were provide by Dr. Wen-Biao Gan at Peking University, Shenzhen Graduate School; Mrp8-Cre mice (B6.Cg-Tg (S100A8-cre,-EGFP) 1Ilw/J) and PhAM^floxed mice (B6; 129S-Gt(ROSA)26Sort^{m1(CAG-COX8A/Dendra2)Dcc}/J) were purchased from The Jackson Laboratory. TSPAN9^−/− mice were provided by Dr. Li Yu at Tsinghua University. All mice were bred and maintained in the specific pathogen-free (SPF) facility at Center for Excellence in Molecular Cell Science, CAS. All experiments were performed with 7- to 12-week-old male mice. Littermates were used as controls whenever possible. Housing conditions for the mice: dark/light cycle 12 h, ambient temperature 20–26 °C, humidity 40–70%. All experimental animal procedures were approved by Institutional Animal Care and Use Committees (IACUCs) of Center for Excellence in Molecular Cell Science, CAS.