Balb c nu nu

PC-10 and PC-10 ΔPDPN cells (1 × 10⁷) in Hank’s Balanced Salt Solution (HBSS) were subcutaneously injected into 4- or 5-week-old female BALB/c-nu/nu (Charles River Laboratories, Yokohama, Japan). After 23 days of cell injection, the mice were euthanized and tumour weights were measured.
For therapeutic experiments, PC-10 cells (5 × 10⁶) in HBSS were subcutaneously injected into 4-or 5-week-old female BALB/c-nu/nu (Charles River Laboratories). After 15 days of cell injection, PC-10 tumour-bearing mice were dosed orally with 50 mg/kg/day erlotinib (LKT Laboratories, Inc., Minnesota, America) for 3 weeks. Clopidogrel sulphate (LC Laboratories, Inc., Alabama, America) was dissolved in drinking water with 0.003% HCl (equal to orally dosed approximately 25 mg/kg/day) for 18 days. When designated treatments were completed, the animals were euthanized and tumours were resected. ChMS-1 antibody treatment was performed as previously described^{5 (link)}.
A549/Neo and A549/PDPN cells (5 × 10⁶) in HBSS were subcutaneously injected into 4- or 5-week old female BALB/c-nu/nu (Charles River Laboratories). After 22 days of cell injections, the mice were euthanized and tumour weights were measured.
All animal procedures were performed using protocols approved by the Japanese Foundation for Cancer Research Animal Care and Use Committee in accordance with the relevant guidelines and regulations.

Female nude mice (Balb/c nu/nu), aged between 4 and 6 weeks, were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). The animals had free access to drinking water and feed ad libitum. All animals were treated in accordance with the procedures outlined in the Guide for the Care and Use of Laboratory Animals (China), and experimental procedures were approved by the Animal Ethics Committee of Zhengzhou University.

Animal experiments were performed following the protocols approved by Institutional Animal Care and Use Committees of Jilin University. Nude (BALB/C-nu/nu) mice and SCID (severe combined immunodeficient) mice were obtained from Vital River Laboratory Animal Technology (Beijing, China). 5×10⁶ PANC-1 cells stably transduced with lentivirus-based pGCSIL-GFP vector carrying negative control and RPL34-siRNA were suspended in HBSS and injected subcutaneously into the flank region of 6-week-old female athymic nude (BALB/C-nu/nu) mice (Vital River Laboratory Animal Technology, Beijing, China). The tumors were monitored and grown to an average volume of 200 mm³. Tumor size was assessed by caliper every other day and tumor volumes were calculated after 24 days using the formula: V = 4/3 × π (length/2 × (width/2)²).
To establish a metastasis model, SCID mice were injected with 1× 10⁶ viable PANC-1-luc-NC and PANC-1-luc-KD cells via tail veins. Successful injection was confirmed by immediate luciferase imaging. For luciferase imaging, mice were anesthetized with isoflurane and intraperitoneally injected with luciferin (25 mg/ml in 0.1 ml PBS). Animals were imaged 15 min after injection using an IVIS LuminaXR system (Caliper, Hopkinton, MA, USA) once a week for a total of six weeks.