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3 protocols using 8 amino 1 octanethiol hydrochloride

1

Synthesis of Thiol-based Molecules

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8-Amino-1-octanethiol hydrochloride (AOT), 6-mercapto-1-hexanol (MOH) and 1-undecanethiol (1-UDT) were purchased from Sigma-Aldrich, 11-amino-1-undecanethiol hydrochloride (AUT) was acquired from Dojindo. All the other chemicals were purchased from Sigma-Aldrich and were of the highest purity available. Solutions were prepared using deionized water form a Milli-Q® Water Purification System (Merck Millipore).
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2

Functionalization of MWCNTs with Diverse Thiol Ligands

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MWCNTs (95%
carbon assay, 6–9 nm × 5 μm (outer diameter ×
length)), ethylenediamine (EDA), N,N′-dicyclohexylcarbodiimid (DCC), copper(I) acetate (Cu(OAc)),
indium(III) acetate (In(OAc)3), zinc acetate (Zn(OAc)2), stearic acid (SA), sulfur (S), 1-dodecanethiol (DDT), 1-octadecene
(ODE), oleylamine (OAm), 8-amino-1-octanethiol-hydrochloride (AOT),
2-(dimethylamino)-ethanethiol hydrochloride (DMA), 2-(diethylamino)-ethanethiol
hydrochloride (DEA), 3-mercaptopropionic acid (MPA), 11-mercaptoundecanoic
acid (MUA), l-glutathione (l-GLU), and tetramethylammonium
hydroxide (TMAH) were all purchased from Sigma Aldrich. Cysteamine
hydrochloride (CYS), conc. nitric acid (HNO3, w = 65%), chloroform (CHCl3), n-hexane,
methanol (MeOH), and toluene were obtained from Carl Roth. All reagents
were used as received without any prior purification step.
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3

Peptide Adsorption on Coated Surfaces

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GL13K (GKIIKLKASLKLL-NH2) was purchased from AAPPTec, LLC (Louisville, KY, USA) with purity >98%. HAP-coated (QSX327) and Au-coated (QSX301) QCM-D sensors were purchased from Biolin Scientific (Gothenburg, Sweden). 1-Hexanethiol (95%), 6-Mercaptohexanoic acid (90%), and 8-Amino-1-octanethiol hydrochloride (95%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). PBS at pH 7.4 (1X) was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
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