Sodium chloride (NaCl, ≥99%) and sodium iodide (NaI, anhydrous, ≥99.5%) were purchased from Sigma-Aldrich. Multi-walled carbon nanotubes (CNT, MR99, average diameter of 10 nm and average length of 10 μm) were purchased from Carbon Nano-material Technology Co. (Korea). Nitric acid (HNO3, 60%), hydrochloric acid (HCl, 36%), acetone (99.7%), ethanol (94.5%), and anhydrous ethanol (99.9%) were purchased from Samchun Chemicals (Korea). Perchloric acid (HClO4, 70%, Veritas double distilled) was purchased from GFS Chemicals. Hydrogen peroxide (H2O2, 30%) was purchased from Junsei Chemical. Carbon paper (TGP-H-60, Toray), Sodium perchlorate monohydrate (NaClO4∙H2O, >97%), standardised sodium thiosulfate solution (Na2S2O3, 0.01 N), and soluble starch powder (ACS, for iodometry) were purchased from Alfa Aesar. Pt(II) meso-tetraphenylporphine (PtTPP, >95%) were purchased from Frontier Scientific. Dihydrogen hexachloroplatinate(IV) hydrate (H2PtCl6∙6H2O, 99.95%) was purchased from Umicore. Natural seawater, obtained from Ilsan beach, Ulsan, Republic of Korea (GPS 35.497005, 129.430996), was used after filtration, whose relative ion concentrations can be found in a previous report67 (link). Commercial dimensionally stable anode (DSA, Ir/Ru atomic ratio = 2) was provided by Siontech Inc. (Korea). All chemicals were used without further purification, except the CNT.
Hydrogen peroxide
Manufactured by Junsei
Sourced in Japan
Hydrogen peroxide is a clear, colorless liquid chemical compound with the formula H2O2. It is a common laboratory reagent used for various purposes, such as oxidation, bleaching, and disinfection.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (3 mentions)
Glucose, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (2 mentions)
Sodium acetate, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
1 1 3 3 tetraethoxypropane, by Merck Group (2 mentions)
Sodium phosphate dibasic, by Merck Group (2 mentions)
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
Sodium hydroxide (naoh), by Daejung Chemicals (2 mentions)
Iron 3 chloride hexahydrate, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Samchun (1 mentions)
Ethanol, by Samchun (1 mentions)
Anhydrous ethanol, by Samchun (1 mentions)
Sodium phosphate monobasic monohydrate, by Merck Group (1 mentions)
D trehalose dihydrate, by Merck Group (1 mentions)
3 3 5 5 tetramethylbenzidine dihydrochloride tmb, by Merck Group (1 mentions)
Sephadex g 15, by Merck Group (1 mentions)
Succinimidyl 4 n maleimidomethyl cyclohexane 1 carboxylate smcc, by Thermo Fisher Scientific (1 mentions)
Ez link nhs lc lc biotin, by Thermo Fisher Scientific (1 mentions)
17 protocols using hydrogen peroxide
1
Synthesis and Characterization of Pt-Based Electrocatalysts
2
Cardiac Troponin I Assay Development
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The stock of a cTn I-T-C complex (SRM 2912) and monoclonal antibodies (clones M18, 560, 19C7, and MF4) specific to cTnI were supplied by Hytest (Turku, Finland). Sodium phosphate dibasic, sodium phosphate monobasic monohydrate, sodium chloride, casein (sodium salt form, extract from bovine milk), 3,3′,5,5′-tetramethylbenzidine dihydrochloride (TMB), Sephadex G-15, and D-(+)–trehalose dihydrate were purchased from Sigma (St. Louis, MO, USA). The NC membrane (HiFlowPlus HFB13504) and polyester membrane were supplied by Millipore (Billerica, MA, USA). EZ-Link NHS-LC-LC-Biotin, succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC), N-succinimidyl 3-(2-pyridyldithio)-propionate (SPDP), dithiotheritol (DTT), goat anti-mouse IgG, and SuperSignal West Femto Chemiluminescent Substrate for HRP were purchased from Thermo Fisher Scientific (Rockford, IL, USA). Streptavidin and HRP were supplied by Calbiochem (San Diego, CA, USA). The cellulose membrane (17CHR, chromatography grade) and the glass fiber membrane for the supply of conjugate were obtained from Whatman (Maidstone, England) and MDI (Ambala Cantt, India) respectively. Hydrogen peroxide, insoluble TMB, and streptavidin-PolyHRP20 (SA-Poly-HRP) conjugate were obtained from Junsei (Tokyo, Japan), Moss (Pasadena, MD, USA), and Fitzgerald (North Acton, MA, USA) respectively. The grade of all reagents was analytical.
3
Electrochemical Bacterial Detection Assay
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Precursor reagents and other chemicals such as graphite powder, zinc acetate, sodium hydroxide, dimethyl sulfoxide (DMSO), 3-aminopropyltriethoxysilane (APTS), and dimethylformamide were commercially obtained from Sigma-Aldrich Co. (St Louis, MO, USA). LIVE/DEAD BacLight kit was used according to manufacturer’s protocol (Molecular Probes; Thermo Fisher Scientific, Waltham, MA, USA). Potassium permanganate and hydrogen peroxide were obtained from Junsei Chemical Co., Ltd (Tokyo, Japan). Sulfuric acid and hydrochloric acid were commercially obtained from Daejung Chemicals and Metal Co., Ltd (Gyeonggi-do, Korea). Milli-Q water with a resistance greater than 18 MΩ was used in all experiments. All of the chemicals were of analytical grade and used as received without further purification. Four pathogenic bacterial strains, namely the Gram-negative strains E. coli (KACC 10005) and S. typhimurium (KCCM 40253), and the Gram-positive strains B. subtilis (KACC 14394) and E. faecalis (KACC 13807) were utilized in the study. The gold printed circuit board (Au-PCB) working electrode used in the cyclic voltammetry (CV) study was made from a conventional Au printed circuit board (PCB) chip.
4
Multimodal Nanoparticle Synthesis and Characterization
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Ferrocenylmethyl methacrylate (FMMA; 95%), methacrylic acid (MA; 99%), PEG methyl ether methacrylate (PEGMA; Mn = 500 g/mol), Nile Red (NR), tetrahydrofuran (THF, anhydrous, 99.9%), and N-acetyl-l -cysteine (NAC) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Hydrogen peroxide (30%) and 2,2-azobisisobutyronitrile (AIBN, 99%) were obtained from Junsei Chemical Co. (Tokyo, Japan) and Daejung (Seoul, Korea), respectively. PTX was obtained from the loading control (LC) Laboratories (Woburn, MA, USA). deionized water (DIW) and phosphate-buffered saline (PBS) were obtained from HyClone (Logan, UT, USA). Acetonitrile (ACN, High Performance Liquid Chromatography (HPLC) grade) was obtained from Honeywell (Charlotte, NC, USA). The fluorescent ROS indicator 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) was obtained from Invitrogen (Carlsbad, CA, USA). Solid DiIC18 (5) (DiD) and Hoechst 33342 were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Paraformaldehyde (4%) and formalin (10%) were obtained from Biosesang (Seongnam, Gyeonggi-do, Korea). The optimal cutting temperature (OCT) compound was obtained from Scigen Scientific Inc. (Gardena, CA, USA).
5
Synthesis of Organic Compounds
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All chemicals were purchased from Sigma–Aldrich or Alfa–Aesar with the highest purity and used without further treatment. Melamine, alkalis chloride and potassium halide, 3,3’,5,5 tetramethylbenzidine dihydrochloride (TMB) were bought from Sigma–Aldrich and used as received. Hydrogen peroxide (35 wt %) was bought from Junsei. Milli-Q water was used for all the experiments.
6
Enzymatic Antioxidant Activity Evaluation
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Barium chloride dihydrate, ethanol, iron (II) sulfate heptahydrate, acetic acid, hydrochloric acid, nitric acid, ammonium sulfate, sodium hydroxide, phenol, and sulfuric acid were purchased from Daejung (Seoul, Korea). From Sigma-Aldrich (St. Louis, MO, USA), 2,2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2-azobis (2-methylpropionamidine) dihydrochloride, 1,1-diphenyl-2-picrylhydrazyl (DPPH), gallic acid, glucose, Folin and Ciocalteu’s phenol reagent, peroxidase, α-(4-pyridyl N-oxide)-N-tert-butylnitrone, 5,5-dimethyl-1-pyrroline-N-oxide, 2′,7′-dichlorofluorescein diacetate (DCF-DA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), and gum arabic were purchased. RPMI-1640 medium, fetal bovine serum, penicillin-streptomycin, and trypsin were purchased from Gibco (Mississauga, ON, Canada). Sodium carbonate anhydrous (Yakuri, Japan), bicinchoninic acid protein assay kit (Thermo Fisher Scientific, Pittsburgh, PA, USA), bovine serum albumin (Bovogen, East Keilor VIC, Australia) and hydrogen peroxide (Junsei, Tokyo, Japan) were used. Commercial food-grade enzymes (alcalase (Al), α-chymotrypsin (α-chy), flavourzyme (Fla), kojizyme (Koj), neutrase (Neu), papain (Pap), pepsin (Pep), protamex (Pro), and trypsin (Try)) were purchased from Novozyme (Bagsvaerd, Copenhagen, Denmark).
7
Nanoceria-based Cholesterol Sensor
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Nanoceria (<5 nm in diameter), cholesterol oxidase from Streptomyces sp. (ChOx), cholesterol, glucose, uric acid, urea, cysteine, xanthine, galactose, triton X-100, sodium acetate, chitosan, succinic acid, aminopropyl triethoxysilane (APTES), and human blood serum were purchased from Sigma-Aldrich (Milwaukee, WI). Hydrogen peroxide (35 %) was obtained from Junsei Chemical Co. (Japan). Whatman grade 1 qualitative filter papers were purchased from GE Healthcare Co. (USA). All other chemicals were of analytical grade or higher and all solutions were prepared with distilled (DI) water purified using a Milli-Q Purification System (Millipore, USA).
8
Antioxidant Capacity Evaluation
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Folin-Ciocalteu’s phenol reagent, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), gallic acid, catechin, nicotinamide adenine dinucleotide, Tris-HCl, and sodium phosphate monobasic were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Hydrogen peroxide was purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan) and 2-thiobarbituric acid was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All chemicals used were of analytical grade.
9
Quantification of Vitamin D2 and Antioxidants
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Vit. D2 (ergocalciferol), quercetin, trifluoroacetic acid, tris-base, sodium acetate, sodium chloride, and hydrochloric acid were purchased from Sigma-Aldrich (St. Louis, MI, USA). Chloroform, ethanol, and methanol were purchased from merckmillipore (Frankfurter Strasse 250 Darmstadt, Germany). Hydrogen peroxide was purchased from Junsei chemical Co., Ltd (Nihonbashi-honcho, Chuo-ku, Tokyo). All other chemicals used for the study but not mentioned here were of the highest analytical grade.
10
Plant Growth Stimulation with Iron and Humic Acid
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Kraft lignin was purchased
from Sigma-Aldrich, whereas lignin from the acid-induced hydrolysis
of wood chips was kindly donated by the GS Caltex Corporation. The
acid lignin containing approximately 1% H2SO4 was directly used without further washing. Plant-stimulation tests
were based on A. thaliana wild-type
(Col-0 background) cultivated on Murashige and Skoog (MS) medium purchased
from Duchefa Biochemie. Iron(II) sulfate heptahydrate, humic acid,
and hydrogen peroxide (35%) were obtained from Junsei, Sigma-Aldrich,
and Duksan, respectively. Additional humic acids used in the ICP-OES
analyses for determining the Fe content originated from China and
the United States and were purchased from MR Innovation Corporation
(Daegu, Korea). Sulfuric acid and 60% perchloric acid were purchased
from Samchun Chemical and J. T. Baker, respectively. Both Folin and
Ciocalteu’s phenol reagent and gallic acid were obtained from
Sigma-Aldrich.
from Sigma-Aldrich, whereas lignin from the acid-induced hydrolysis
of wood chips was kindly donated by the GS Caltex Corporation. The
acid lignin containing approximately 1% H2SO4 was directly used without further washing. Plant-stimulation tests
were based on A. thaliana wild-type
(Col-0 background) cultivated on Murashige and Skoog (MS) medium purchased
from Duchefa Biochemie. Iron(II) sulfate heptahydrate, humic acid,
and hydrogen peroxide (35%) were obtained from Junsei, Sigma-Aldrich,
and Duksan, respectively. Additional humic acids used in the ICP-OES
analyses for determining the Fe content originated from China and
the United States and were purchased from MR Innovation Corporation
(Daegu, Korea). Sulfuric acid and 60% perchloric acid were purchased
from Samchun Chemical and J. T. Baker, respectively. Both Folin and
Ciocalteu’s phenol reagent and gallic acid were obtained from
Sigma-Aldrich.
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