Transdetect in situ fluorescein tunel apoptosis detection kit

TUNEL assay (TransDetect^® In Situ Fluorescein TUNEL Apoptosis Detection Kit; TransGen Biotech, Beijing, China) was performed according to the manufacturer’s instructions. Briefly, the sectioned slides were incubated with xylene and in a descending ethanol series (100%, 95%, 80%, and 70%) for deparaffinization and washed with distilled water. Then, the slides were rinsed with phosphate-buffered saline (PBS) and incubated with permeabilization solution for 5 min at room temperature. After incubation with labeling solution for 1 h at 37 °C, the labeled slides were rinsed with permeabilization solution 3 times. The washed slides were incubated with 4’,6-Diamidino-2-Phenylindole, dihydrochloride (DAPI), and mounted with VECTASHIELD (Vector Laboratories Inc.) mount.

The TransDetect^® In Situ Fluorescein TUNEL Apoptosis Detection Kit was purchased from Transgen Biotech (Beijing, China) and used according to the manufacturer’s instructions. The fluorescein-labeled DNA was detected via fluorescence microscopy.