We established PD model by 6-Hydroxydopamine (6-OHDA) according to Nass’s report [49 (link)]. About 150 L3 stage worms were harvest, washed with M9 buffer, and added to a mixture including 10 mM ascorbic acid and 50 mM 6-OHDA. The worms were treated for 1 h at 24 °C, mixed mildly every 15 min, washed with M9 buffer. After the PD model was successfully established, the animals were divided into two groups including PD model group and gastrodin group. The animals of PD model group were laid in OP50/NGM medium addition with no gastrodin for 24 h, then treated with floxuridine (purchased from Sigma, F0503) in 22 °C; The animals of gastrodin group were laid in OP50/NGM medium addition with gastrodin for 24 h, then treated with gastrodin and floxuridine in 22 °C. Both of the control group and gastrodin group were observed and further test after 72 h in Fig. 1 a. Every experiment was performed at least three independent replicates.
Floxuridine
Manufactured by Merck Group
Sourced in United States
Floxuridine is a laboratory compound used in research and development. It is a synthetic nucleoside analog that can be used as a research tool. The core function of Floxuridine is to inhibit DNA synthesis, which can be utilized in various scientific experiments and investigations.
Automatically generated - may contain errors
Lab products found in correlation
5 ethynyl 2 deoxyuridine edu, by Thermo Fisher Scientific (1 mentions)
2 deoxy 5 fluoro uridine, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Thymidine, by Merck Group (1 mentions)
Cyclopamine, by Merck Group (1 mentions)
Sunitinib, by Merck Group (1 mentions)
Ribavirin, by Merck Group (1 mentions)
Dasatinib, by Merck Group (1 mentions)
Ifosfamide, by Merck Group (1 mentions)
Vinblastine, by Merck Group (1 mentions)
Fluorouracil, by Merck Group (1 mentions)
Bisindolylmaleimide, by Cayman Chemical (1 mentions)
Cilostazol, by Merck Group (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Docetaxel, by Merck Group (1 mentions)
Imatinib, by Cayman Chemical (1 mentions)
Bicalutamide, by Merck Group (1 mentions)
Salinomycin, by Merck Group (1 mentions)
4 protocols using floxuridine
1
Parkinson's Disease Model in C. elegans
2
Monitoring DNA Repair Kinetics via EdU
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Cells were cultured on 24-mm cover slips and were serum starved (0.5% fetal calf serum) for 2 days to accumulate cells in G0 phase (∼98%). After damage induction, cells were directly labeled with EdU for 3 h (wt cells) or 7 h (GG-NER deficient cells) using Ham's F10 supplemented with 0.5% dialyzed fetal calf serum containing 20 μM 5-ethynyl-2΄-deoxyuridine (EdU, ThermoFisher Scientific). 2΄-Deoxy-5-fluorouridine (1 μM) (Floxuridine, Sigma-Aldrich) was added to inhibit the thymidylate synthase to prevent the generation of endogenous thymidine. After Edu labeling, medium was changed to Ham's F10 supplemented with 0.5% dialyzed fetal calf serum containing 10 μM thymidine (Sigma-Aldrich) for 15 min to deplete unincorporated EdU in the cell. Cells were fixed by incubation with 3.6% formaldehyde (Sigma-Aldrich) in PBS with 0.5% Triton X-100 (Sigma-Aldrich) for 15 min at room temperature. After permeabilization in 0.5% Triton for 20 min, cells were blocked with 3% BSA (Sigma-Aldrich) in PBS.
3
Comprehensive Pharmacological Screening Protocol
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The following 43 reagents were used in this study: AMG 102 and panitumumab (Amgen); cercosporamide (BioAustralis); AKT inhibitor V (Calbiochem); AS-252424, bisindolylmaleimide, CGP 57380 and imatinib (Cayman Chemical); CTX ILK inhibitor (CRC); Avastin (bevacizumab) (Genentech/Roche); 4EGI-1, ABT-737, ABT-737 enantiomer, pazopanib, and retinoic acid (Santa Cruz Biotechnology); erlotinib, lapatinib, sorafenib, and temsirolimus (Scientifix); bortezomib and CYT387 (Selleck Chemicals); AKT-I-1/2, axitinib, bicalutamide, cilostazol, cyclopamine, DAPT, dasatinib, docetaxel, doxorubicin, floxuridine, fluorouracil, goserelin, ifosfamide, PD98059, ribavirin, salinomycin, SU11274, sunitinib, Tamoxifen, and vinblastine (Sigma); NSC 7908 (Tocris); and temozolomide (Wyeth).
4
Fluorescent Imaging Reagents Protocol
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Floxuridine and GA were obtained from Sigma (St. Louis, MO, USA). FUdR was stored as 2 mm stocks in ultrapure water at −20 °C. GA was stored as 2 mm stock in dimethyl sulfoxide at −20 °C with protection from light. 4’,6‐Diamidino‐2‐phenylindole dihydrochloride was obtained from Invitrogen (Carlsbad, CA, USA).
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