Starting on day 3 or day 5 post‐infection, mice were imaged three times a week for 2 weeks then once a week until no above‐background luciferase signal was detected for at least two sequential imaging sessions. Ten minutes prior to imaging, mice were administered D‐luciferin, potassium salt (GoldBio Cat#LUCK1G) via the intraperitoneal route. Stock solutions of D‐luciferin were prepared in DPBS at 15 mg/ml and each mouse received 150 mg luciferin/kg body weight. During each imaging session, mice were anesthetized with isoflurane and imaged in groups of three for 1 min using the In‐Vivo Xtreme II system (Bruker) or the IVIS Spectrum CT (Perkin‐Elmer). Contrast levels of X‐ray images were adjusted using MI software (Bruker). Scaling of the luminescence overlays was standardized for all groups of mice and mean luminescence intensity (MLI) or average radiance values were calculated using MI software (Bruker) or Living Image software (Perkin‐Elmer). Images were exported to Adobe Photoshop for cropping.
Mi software
Manufactured by Bruker
MI software is a data analysis and processing tool designed for Bruker's scientific instruments. It provides users with a platform to manage, visualize, and analyze data acquired from Bruker's analytical equipment.
Automatically generated - may contain errors
Lab products found in correlation
In vivo xtreme imaging system, by Bruker (2 mentions)
Female sho mice, by Charles River Laboratories (2 mentions)
In vivo ms fx pro, by Bruker (2 mentions)
In vivo fx pro, by Bruker (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
Fx pro, by Carestream (1 mentions)
Luck 1g, by GoldBio (1 mentions)
In vivo xtreme 2 system, by Bruker (1 mentions)
Living image software, by PerkinElmer (1 mentions)
Ivis spectrum ct, by PerkinElmer (1 mentions)
13 protocols using mi software
1
In Vivo Bioluminescence Imaging of Mice
2
In vivo Optical Imaging of Nectin-4 Tumor Targeting
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MDA-MB-468 xenograft tumor-bearing mice were administrated intravenously with 200 μL mAbNectin-4-ICG, free ICG (1 mg/kg ICG dose), or saline. Mice were anesthetized by 1% pentobarbital sodium during imaing process. The in vivo fluorescence (FL) imaging at multiple time points (3, 6, 12, 24, 36, and 48 h) was achieved with IVIS Spectrum imaging system with Excitation/Emission of 750/790 nm) and analyzed using Bruker MI software. The ROIs of the tumor area (tumor) and the lower limb muscle (muscle) were delineated and the corresponding FL intensity was obtained. The T/M ratio was calculated as Intensitytumor/Intensitymuscle. After the last scanning time point, all mice were killed to collect the tumors and major organs to conduct the ex vivo FL imaging. The intensity of each organ and tumors were measured for quantification and comparison.
3
Quantitative Bioluminescent Imaging of Ovarian Tumor Burden
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The Yale Institutional Animal Care and Use Committee approved all animal protocols that guided the in vivo experiments described in this study. All animals bear i.p. tumors from TKO ovarian cancer cells. 1 × 107 (link) mCherry-positive TKO cells were injected in 4-week old female C57BL/6 J mice (Jackson Laboratories). Imaging was performed twice a week under isoflurane anesthesia using the Invivo FX PRO imaging system (Bruker Corp.). mCherry fluorescence was acquired at 550 excitation and 635 emission. Images were set at 0.5 min and 5000 max and mCherry region of interest (ROI) area was quantified as previously described using Bruker MI software.34 (link),35 (link) Body area, which was used as a surrogate for abdominal girth, was calculated using height and width measures from x-ray images. Body area measurement that is equal to or greater than 800 mm2 was considered to be positive for ascites.
4
Multimodal Imaging of Tumor Uptake
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Fluorescence and X-ray images were acquired with the optical/X-ray Imaging System In-Vivo Xtreme (BVX, Bruker Corp. USA) to verify tumor uptake 24 h and 48 h after the intravenous injection of IntegriSense™680. Ex vivo images of the tumor were also acquired. Fluorescence images were obtained with an exposure time of 2 s; the binning was 4 × 4 and the field of view (FOV) was 12.5 cm. IntegriSenseTM 680 excitation and emission wavelengths were 630 and 700 nm respectively. The X-ray parameters were set as follows: energy of 45 kVp, current of 497 μA, and an aluminum filter of 0.8 mm. The exposure time was 2 s, a 1 × 1 binning, and a FOV of 12.5 cm. Using the Bruker’s MI software, the fluorescence and X-ray images were overlapped. Due to the variability of tumor morphology, the regions of interests (ROIs) were drawn freehand and quantified in terms of mean photons/s/mm2.
5
Near-Infrared Fluorescence Imaging of Drug Delivery
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The near-infrared (NIR) fluorescence imaging is able to provide intuitive understanding with respect to the distribution of drug delivery system in tumor-bearing mice. In this study, a Bruker in vivo imaging system (FXPro; Carestream Health, Rochester, NY, USA) was used. The excitation wavelength was set at 670 nm and the emission at 700 nm. Prior to imaging experiments, the mice with S180 tumors ranging from 180 mm3 to 220 mm3 were intravenously administrated with MnO2/HA/Cy 5.5 and free Cy 5.5 formulation, which was prepared in Cremophor polyoxyl 35 castor oil/ethanol/5% of glucose (v/v/v, 1:1:9). The administrated dose of the probe was set at 4.8 μg/kg. At the scheduled time, the anesthetized animals were laid on a removable tray equipped with the imaging cabinet. The NIR fluorescence images and X-ray images were recorded by applying the custom capture settings. The captured images were processed with the Bruker MI software.
Three tumor-bearing mice were involved in the MRI. Two of them were administrated with MnO2/HA/CDDP and the other was the control.
Three tumor-bearing mice were involved in the MRI. Two of them were administrated with MnO2/HA/CDDP and the other was the control.
6
Cy-peptide Analogues Biodistribution in Mice
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Female SHO mice (catalog no. 474; Charles River Laboratories) were administered with free Cyanine5.5 or Cy-peptide analogues (0.5 nmol of Cyanine5.5 content measured by UV absorbance at 680 nm) in a PBS solution (150 μL) via tail-vein injections (n = 4/group). Real-time fluorescence imaging was performed using an In Vivo Xtreme imaging system (Bruker). Whole body fluorescence images were acquired 1 and 4 hours postinjection using the appropriate excitation (670 nm) and emission (750 nm) filters. The animals were then euthanized. The organs were excised to perform ex vivo fluorescence imaging. Imaging was also performed on urine samples (20 μL) collected from separate animals 1, 4, and 6 hours after intravenous injection of free dye or Cy-peptide analogues (n = 4/treatment). Bruker MI software was used to process the fluorescence/bright light images and measure the fluorescence intensity in different ROIs. All the data were corrected to eliminate the organ or fluid auto-fluorescence.
7
In Vivo Multimodal Imaging Protocol
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Live in vivo x-ray and fluorescence images were obtained using In Vivo MS FX PRO (Bruker, Inc., Billerica, MA) in mice that were administered 2% isoflurane. Quantitative analysis of the optical signal capture was performed using Bruker MI Software. Fluorescence filters were as follows: (1) mCherry excitation = 550 and emission = 635; (2) DIR excitation = 760 and emission = 700; and (3) Coumarin 6 excitation = 450 and emission = 700. Fluorescence intensity for each of the probes was determined and reported as mean fluorescent intensity (MFI). Images were overlaid and analyzed for colocalization (Pearsons’s correlation coefficient) using ImageJ software (v 2.0). For all analysis, the investigators were not blinded to the group allocation.
8
In vivo Optical Imaging of Nectin-4 Tumor Targeting
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MDA-MB-468 xenograft tumor-bearing mice were administrated intravenously with 200 μL mAbNectin-4-ICG, free ICG (1 mg/kg ICG dose), or saline. Mice were anesthetized by 1% pentobarbital sodium during imaing process. The in vivo fluorescence (FL) imaging at multiple time points (3, 6, 12, 24, 36, and 48 h) was achieved with IVIS Spectrum imaging system with Excitation/Emission of 750/790 nm) and analyzed using Bruker MI software. The ROIs of the tumor area (tumor) and the lower limb muscle (muscle) were delineated and the corresponding FL intensity was obtained. The T/M ratio was calculated as Intensitytumor/Intensitymuscle. After the last scanning time point, all mice were killed to collect the tumors and major organs to conduct the ex vivo FL imaging. The intensity of each organ and tumors were measured for quantification and comparison.
9
Biodistribution of Cationic Nanomaterials in CIA Rats
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At day 30 after the first immunization, the untreated CIA rats and normal rats were anaesthetized (1.5–2% isoflurane, 0.5 ml/min oxygen) and the Alexa Fluor® 750 labeled cationic materials were intravenously injected. The biodistribution of the materials in four groups, including PDMA-treated normal group, PDMA-treated model group, cNP-treated normal group, and cNP-treated model group, was monitored by NIRF imaging using an in vivo imaging scanner (Carestream FX PRO) at different time points during 24 h. At 4 and 24 h, one from each group was sacrificed for dissection. The joints, thymus, heart, lung, liver, kidney, pancreas, spleen, and bladder were taken out for ex vivo NIRF imaging and their mean NIRF intensity was calculated by Bruker MI software.
10
In Vivo Fluorescence Imaging of Cyanine5.5 and Cy-peptide Analogues
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