Tecnai g2 20

Manufactured by Thermo Fisher Scientific

Sourced in United States, Netherlands, United Kingdom, Germany, Canada

The Tecnai G2 20 is a high-performance transmission electron microscope (TEM) designed for advanced materials analysis and research. It features a 200 kV electron beam with high-resolution imaging capabilities. The core function of the Tecnai G2 20 is to provide detailed structural and compositional information about a wide range of materials at the nanoscale level.

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Spelling variants of this product

Tecnai G2 (325 citations) Tecnai 20 (109 citations) Tecnai G2 20 TWIN (101 citations) FEI/TECNAI G2 20 (13 citations) Tecnai G2 20S-Twin transmission electron microscope (11 citations) Tecnai G2 20 S-twin instrument (3 citations) Tecnai G2-20—FEI SuperTwin 200 kV (3 citations) Tecnai G2 20S-Twin 200 kV (2 citations) Tecnai™ G2 20 U-Twin (2 citations) Tecnai G2 20 TWIN instrument (2 citations)

192 protocols using tecnai g2 20

Cryo-EM Analysis of RNF213 Protein

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The samples were diluted to 0.05–0.1 mg/mL and applied onto carbon-coated Cu/Pd Hexagonal 400 mesh grids (Agar Scientific, #G2440PD). Prior to application of the sample, the grids were glow-discharged on a glass plate with a current of 20 mA for 60 s in the SCD 005 Sputter Coater (BAL-TEC) to clean and hydrophilize the surface. The grids were screened to assess sample quality on a FEI Morgagni microscope equipped with a Morada camera (Olympus-SIS) or a FEI Tecnai G2 20 microscope equipped with a 4 k Eagle camera (FEI). Additionally, promising negative stain grids were imaged on the FEI Tecnai G2 20 using SerialEM (Schorb et al., 2019 (link)) with a defocus range of –1.5 µm to –2.5 µm and a pixel size of 1.85 Å/px. Negative staining micrographs were analyzed using Relion 2.1 (Scheres, 2012 (link)). No CTF correction was applied to the images. Around 5000 individual particles were manually picked, from which 2D class averages were generated and used for auto-picking the entire dataset. This dataset was again subjected to 2D classification to assess the conformational variability of RNF213 particles.

Ahel J., Lehner A., Vogel A., Schleiffer A., Meinhart A., Haselbach D, & Clausen T. (2020). Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism. eLife, 9, e56185.

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Characterization of Nanoparticle Size and Zeta Potential

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The size and zeta potential of NPs were examined using Malvern Mastersizer 2000 Particle Analyzer (Zetasizer Nano ZS90, Malvern Instruments Ltd., UK). The freshly prepared NPs were diluted with distilled water. After equilibration for 10 min, the detections were performed at 25 °C. The data were obtained with the average of three measurements.
The morphology of NPs was characterized by transmission electron microscopy (TEM, Tecnai G2 20, FEI Company, Hillsboro, Oregon, USA). The NPs power was suspended by deionized water at first. Then the solution of NPs was dropped onto a copper grid coated with a carbon membrane and dried at room temperature before observing the TEM images.

Gong X., Zheng Y., He G., Chen K., Zeng X, & Chen Z. (2019). Multifunctional nanoplatform based on star-shaped copolymer for liver cancer targeting therapy. Drug Delivery, 26(1), 595-603.

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Ultrastructural Analysis of Eye Tissues

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Electron microscopy analysis was performed as described previously [53 (link)]. Briefly, eye samples were enucleated, and the anterior segment and the lens were removed. Eye cups were fixed in fixative buffer (4% paraformaldehyde and 3% glutaraldehyde for 4 h in 0.1 M sodium-phosphate buffer, pH 7.4) at 4 °C for at least 24 h, and post-fixed in 1% osmium tetroxide for 2 h at 4 °C. After a stepwise ethanol and acetone dehydration and infiltration with Spurr’s epoxy resin, the samples were embedded and polymerized in Spurr’s epoxy resin at 60 °C for 48 h. Then, the samples were sectioned at a thickness of 70 nm using an ultramicrotome (EM UC7, Leica). The sections were contrasted with 5% uranyl acetate and lead citrate and examined under a transmission electron microscope (Tecnai G² 20, FEI, Oregon, USA).

Li Z., Li H., Xu X., Wang L., Liu B., Zheng W., Lian L., Song Y., Xia X., Hou L., Cheng H, & Zhou R. (2019). Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina. Cell Death and Differentiation, 27(2), 556-572.

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Characterization of Macromolecular Samples

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The SEC-System (Pharmacia, Uppsala, Sweden) was equipped with a prepacked Superdex 200 10/300 GL size exclusion column (GE Healthcare Bio-Sciences, Uppsala, Sweden). The gas-phase electrophoretic mobility macromolecular analyzer (GEMMA TSI Inc., Shoreview, MN, U.S.A.) instrument consisted of an electrospray aerosol generator, an electrostatic classifier control unit equipped with a nano differential mass analyzer (nano DMA) and an ultrafine condensation particle counter (CPC) for detecting the analyte or alternatively an electrostatic nano particle sampler (ENPS) for collecting the analyte on a selected substrate. A fused-silica capillary with an ID of 25 μm (polyimide coated, OD: 150 μm) was used for the spray process. The AFM images were recorded on a NanoScope III Multimode SPM instrument (Veeco Instruments, Santa Barbara, CA, U.S.A.) using silicon cantilevers with integrated silicon tips (NanoWorld, Neuchâtel, Switzerland, Arrow type: NC) and mica platelets for AFM (Plano, Wetzlar, Germany). Transmission electron microscopy was performed on a Tecnai G2 20 instrument (FEI, Hillsboro, OR, U.S.A.) on 300 mesh copper grids with carbon and Formvar coating purchased from Plano (Wetzlar, Germany).

Havlik M., Marchetti-Deschmann M., Friedbacher G., Winkler W., Messner P., Perez-Burgos L., Tauer C, & Allmaier G. (2015). Comprehensive Size-Determination of Whole Virus Vaccine Particles Using Gas-Phase Electrophoretic Mobility Macromolecular Analyzer, Atomic Force Microscopy, and Transmission Electron Microscopy. Analytical chemistry, 87(17), 8657-8664.

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Comprehensive Characterization of LNSD and TD

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DLS (Zetasizer Nano ZS-90, Malvern, UK), TEM (Tecnai G2 20, FEI) and AFM (SPM-9700, Shimadzu, Japan) were used for characterizing zeta potential, particle size and morphology of LNSD and TD, respectively. Polyacrylamide gel electrophoresis (PAGE, 8%) was used to verify TD and TD/DOX.

Li Y., Zhai Y., Liu W., Zhang K., Liu J., Shi J, & Zhang Z. (2019). Ultrasmall nanostructured drug based pH-sensitive liposome for effective treatment of drug-resistant tumor. Journal of Nanobiotechnology, 17, 117.

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Characterization of PDA-Coated Carbon Nanotubes

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The morphology of CNTs and PDA@CNTs was observed by transmission electron microscopy (TEM, Tecnai G2 20, FEI). The specimens were dispersed in ethanol under ultrasonication, and one drop of the dispersion was placed on the carbon-coated copper grid and then dried at 50 °C. The chemical components and functional groups were investigated by Fourier transform infrared (FTIR) and Raman spectroscopy; FTIR spectra were recorded using a VERTEX 70 FTIR spectrometer (Bruker, Germany) in the range of 600–4000 cm⁻¹. Raman spectra were obtained using LabRAM HR800 (Horiba Jobin Yvon, USA) with a 532 nm Nd-YAG laser at 50 mW in the range of 400–4000 cm⁻¹. The percentage of PDA on CNTs was estimated by thermogravimetric analysis (TGA, Pyris1 TGA, PerkinElmer Instruments), in which a 10 mg PDA@CNTs specimen was heated from 40 °C to 800 °C at the rate of 10 °C min⁻¹ under a nitrogen atmosphere.

Cai G., Hou J., Jiang D, & Dong Z. (2018). Polydopamine-wrapped carbon nanotubes to improve the corrosion barrier of polyurethane coating. RSC Advances, 8(42), 23727-23741.

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Transmission Electron Microscopy of 3T3-L1 Cells

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For transmission electron microscopy, undifferentiated or differentiated 3T3-L1 cells (ntc, shPex16, si-ctrl, and si-Pex16) were cultured on an Aclar film, fixed in 0.1 M phosphate buffer (pH 7.4) containing 2.5% glutaraldehyde and 2% formaldehyde (2 h), post-fixed in 2% OsO4 (2 h), dehydrated in graded series of ethanol, and embedded in a TAAB epoxy resin. Images of 70 nm sections (stained with lead citrate and platine blue) were taken on a FEI Tecnai G2 20 transmission electron microscope (FEI, Eindhoven, Netherlands) with a Gatan ultrascan 1000 CCD camera (acceleration voltage 120 kV). Peroxisomes and mitochondria were counted in ≥35 images per biological replicate.

Hofer D.C., Pessentheiner A.R., Pelzmann H.J., Schlager S., Madreiter-Sokolowski C.T., Kolb D., Eichmann T.O., Rechberger G., Bilban M., Graier W.F., Kratky D, & Bogner-Strauss J.G. (2016). Critical role of the peroxisomal protein PEX16 in white adipocyte development and lipid homeostasis. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1862(3), 358-368.

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Multi-Technique Characterization of Functional Materials

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Fourier transform
infrared (FT-IR) spectra were recorded on a PerkinElmer spectrum100
spectrophotometer. The crystallinity of the samples was analyzed by
powder X-ray diffraction (XRD) using a Bruker D8 advance diffractometer
with Cu Kα radiation (1.5418 Å), and the patterns were
recorded in the 2θ range of 5–70°. N₂ adsorption/desorption isotherms were conducted on a Quantachrome
instrument (Quantachrome Instruments, Boynton Beach, USA). The sample
morphology was examined using a scanning electron microscope (SEM,
Hitachi S4800) and transmission electron microscope (TEM; FEI Tecnai
G2 20, accelerating voltage of 200 kV). The acidic properties of samples
were examined via an NH₃-TPD (Micromeritics AutoChem II
2920) instrument. Thermogravimetric (TG) analysis was carried out
using a NETZSCH/STA 409 PC Luxx simultaneous thermal analyzer at a
heating rate of 5 °C/min.

Zhang Q., Yang T., Lei D., Wang J, & Zhang Y. (2020). Efficient Production of Biodiesel from Esterification of Lauric Acid Catalyzed by Ammonium and Silver Co-Doped Phosphotungstic Acid Embedded in a Zirconium Metal–Organic Framework Nanocomposite. ACS Omega, 5(22), 12760-12767.

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Ultrastructural Analysis of Mid-Intestine

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The mid-intestinal tracts were prepared for TEM by fixation in 2.5% glutaraldehyde for 4 h at 4 °C, post-fixation for 3 h in 1% (w/v) osmic acid dissolved in PBS and embedding the tracts in Epon. Finally, the prepared ultrathin sections were examined by TEM (Tecnai G220, FEI, USA). The space between the tight junctions was assessed by Image pro-plus6.0. Six sections from the respective six fish were randomly selected to measure the space between tight junctions for each group.

Kong W., Huang C., Tang Y., Zhang D., Wu Z, & Chen X. (2017). Effect of Bacillus subtilis on Aeromonas hydrophila-induced intestinal mucosal barrier function damage and inflammation in grass carp (Ctenopharyngodon idella). Scientific Reports, 7, 1588.

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Transmission Electron Microscopy of First Trimester Placenta

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For transmission electron microscopy, first trimester placenta was fixed in 0.1 M phosphate buffer (pH 7.4) containing 2.5% glutaraldehyde and 2% formaldehyde (2 h), post-fixed in 2% OsO4 (2 h), dehydrated in graded series of ethanol, and embedded in a TAAB epoxy resin (Gröpl). Ultrathin sections (75 nm) were cut with a Leica UC 7 and stained with lead citrate and platine blue. Images were taken on a FEI Tecnai G2 20 transmission electron microscope (FEI, Eindhoven, the Netherlands) with a Gatan ultrascan 1000 CCD camera (acceleration voltage 120 kV.

Gauster M., Maninger S., Siwetz M., Deutsch A., El-Heliebi A., Kolb-Lenz D., Hiden U., Desoye G., Herse F, & Prokesch A. (2017). Downregulation of p53 drives autophagy during human trophoblast differentiation. Cellular and Molecular Life Sciences, 75(10), 1839-1855.

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