Hrp conjugated anti mouse igg whole molecule

Manufactured by Merck Group

HRP-conjugated anti-mouse IgG (whole molecule) is a laboratory reagent used for immunodetection assays. It consists of anti-mouse IgG antibodies conjugated to horseradish peroxidase (HRP), which can be used to detect the presence of mouse immunoglobulin G (IgG) in samples.

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Lab products found in correlation

Ab56676, by Abcam (1 mentions) Ab24170, by Abcam (1 mentions) Biospot reader, by Cellular Technology (1 mentions) Saponin, by Merck Group (1 mentions)

Close spelling variants of this product

HRP anti-mouse (6 citations) Whole mouse IgG (5 citations) Anti-IgG mouse (5 citations) IgG mouse (3 citations)

2 protocols using hrp conjugated anti mouse igg whole molecule

Immunoblotting and Immunofluorescence Assays

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The goat anti-CatD antiserum was purchased from Santa Cruz Biotechnology (sc-6487 Dallas, TX, USA), and used 1:1000 in PBS for immunoblotting (IB) and 1:150 for immunofluorescence (IFI). The rabbit anti-CD-MPR antiserum was gently provided by Dr. Luzio (Cambridge University, UK), and used 1:250 for IB and 1:200 for IFI. The rabbit anti-CI-MPR antiserum was gently provided by Dr. Nancy Dahms (Medical College of Wisconsin, USA) and used 1: 500 for IB and 1:100 for IFI. The rabbit anti-LAMP1 antiserum (ab-24170) and mouse anti-β-tubulin monoclonal antibody (ab-56676) were obtained from Abcam (USA). The mouse anti-golgin97 monoclonal antibody was obtained from Santa Cruz Biotechnology (sc-73619). The HRP-conjugated anti-goat IgG antiserum was obtained from H&L (401515), the HRP-conjugated anti-rabbit IgG fraction was obtained from Sigma (A9169) and the HRP-conjugated anti-mouse IgG (whole molecule) was purchased from Sigma (A9044). Chemiluminescent reagents were from Pearce (Rockford, IL, USA).

Bannoud N., Carvelli F.L., Troncoso M., Sartor T., Vargas-Roig L.M, & Sosa M. (2018). Cation-dependent mannose-6-phosphate receptor expression and distribution are influenced by estradiol in MCF-7 breast cancer cells. PLoS ONE, 13(8), e0201844.

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Viral Focus-Forming Assay in Vero Cells

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Vero cells were plated in 96-well flat bottom plates. One day later, viruses were serially diluted 10-fold and added to cell monolayers. After 1 hour at 37°C, 1% (w/v) methylcellulose in MEM overlay was added. Cells were incubated for 2 days, then fixed with 1% paraformaldehyde diluted in PBS for 1 h at room temperature. Cells were then stained with primary antibody: 1 μg/ml of E60 for either 2 h at room temperature or overnight at 4°C and then secondary antibody: 1:2000 of HRP-conjugated anti-mouse IgG whole molecule (Sigma) for 1 h at room temperature; both stains are diluted in PBS with 0.1% (w/v) saponin (Sigma) and 0.1% BSA. Foci were developed with TrueBlue peroxidase (KPL) and counted on a BioSpot reader (Cellular Technology Limited).

Chen R.E., Smith B.K., Errico J.M., Gordon D.N., Winkler E.S., VanBlargan L.A., Desai C., Handley S.A., Dowd K.A., Amaro-Carambot E., Cardosa M.J., Sariol C.A., Kallas E.G., Sékaly R.P., Vasilakis N., Fremont D.H., Whitehead S.S., Pierson T.C, & Diamond M.S. (2021). Implications of a highly divergent dengue virus strain for cross-neutralization, protection, and vaccine immunity. Cell host & microbe, 29(11), 1634-1648.e5.

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