Xterra ms c18 column

Manufactured by Agilent Technologies

The XTerra MS-C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. It features a silica-based stationary phase with C18 functional groups, which provides excellent retention and selectivity for a variety of analytes, including polar, non-polar, and ionizable compounds. The column is suitable for use in various HPLC applications, including pharmaceutical, environmental, and food analysis.

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Lab products found in correlation

Lc2695, by Waters Corporation (1 mentions) Prisma ht buffer, by Pion Inc (1 mentions) Agilent 1100 hplc system, by Agilent Technologies (1 mentions) 1290 uhplc system, by Agilent Technologies (1 mentions) Masshunter workstation software, by Agilent Technologies (1 mentions) Agilent 1260 infinity system, by Agilent Technologies (1 mentions)

Close spelling variants of this product

XTerra C18 column C18 column

4 protocols using xterra ms c18 column

RP-HPLC Analysis of P. harmala Alkaloids

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The analysis of P. harmala alkaloids was performed, according to our previously reported method,^{16 (link)} by RP-HPLC using a Waters LC 2695 coupled to
a Waters 996 diode array detector (DAD). A 100 × 4.6 mm² id, 5 μm, XTerra MS-C18 column (Agilent Technologies) was
used for analysis. Chromatographic conditions were water supplemented
with triethylamine (pH 8.6) (A) and acetonitrile (B). The gradient
was programmed from 0% B (100% A) to 20% B in 4 min, then 30% B at
9 min, 50% B at 14 min, and finally to 60% B at 16 min. The separation
was followed by a 2 min washing procedure with 100% B and a reequilibration
period of 4 min. Flow rate: 1 mL/min, column temperature: 25 °C,
injection volume: 20 μL, and absorbance detection: 320 nm. Under
these conditions, the elution order was peganine, harmol, and harmaline.
These peaks were compared to standard harmine, and the isolated alkaloids
were peganine, harmol, and harmine when injected individually on HPLC
(Figure 1). The isolated
ones were annotated by their mass and UV spectra (Figures S1 and S2). Calibration curves were constructed for
peganine, harmol, and harmine to be used in the measurement of encapsulation
efficiency and in vitro release studies.

Fahmy S.A., Issa M.Y., Saleh B.M., Meselhy M.R, & Azzazy H.M. (2021). Peganum harmala Alkaloids Self-Assembled Supramolecular Nanocapsules with Enhanced Antioxidant and Cytotoxic Activities. ACS Omega, 6(18), 11954-11963.

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In Vitro Permeability of Quinolones

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In vitro permeability through the lipid membrane model of selected QNs was investigated using the BBB-PAMPA assay. Briefly, 100 μmol/L donor solutions of the selected QNs and reference drugs were prepared in Pion's Prisma HT® buffer (pH = 7.4). “Brain sink buffer” (Pion, USA) was used as the acceptor fluid. The PAMPA sandwich was incubated for 4 h at 25 °C before sample analysis. All samples were analyzed with an Agilent 1100 HPLC system with an Xterra MS C18 column (3.5 μm, 100 mm × 4.6 mm) at 50 °C and 1% ammonium phosphate buffer at pH = 2.5 (A) with MeCN (B) as the mobile phase. The percentage of B for isocratic elution: 34%, 19%, 31%, 19%, 14%, 26%, 30% and 6% for 15, 18, 19, 32, lidocaine, sulfasalazine, haloperidol and theophylline, respectively, while the detection wavelengths were 246, 302, 296, 262, 230, 362, 246 and 272 nm, respectively. The negative logarithm of the effective permeability (–logP_e) under sink conditions was calculated using the permeability equation⁸² . Permeability was binned based on the reference drugs: CNS+, –logP_e < 5.6, high; CNS–, –logP_e > 6.3, low; intermediate values were designated as intermediate permeability.

Knez D., Diez-Iriepa D., Chioua M., Gottinger A., Denic M., Chantegreil F., Nachon F., Brazzolotto X., Skrzypczak-Wiercioch A., Meden A., Pišlar A., Kos J., Žakelj S., Stojan J., Sałat K., Serrano J., Fernández A.P., Sánchez-García A., Martínez-Murillo R., Binda C., López-Muñoz F., Gobec S, & Marco-Contelles J. (2023). 8-Hydroxyquinolylnitrones as multifunctional ligands for the therapy of neurodegenerative diseases. Acta Pharmaceutica Sinica. B, 13(5), 2152-2175.

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UHPLC-QTOF-MS Analysis of Compounds

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Both chromatographic conditions and mass spectrometer parameters were previously described in other publication [29] (link). Briefly, analysis was performed on Agilent 1290 UHPLC system with XTerra MS C18 Column (30 mm×2.1 mm; 3.5 µm). The mobile phase A was ultrapure water with 0.05% FA and B was MeOH at a flow rate 0.4 ml min⁻¹ in gradient elution (95% A to 0% A in 15 min, back to 95% A in 1 min and kept at 95% A for 5 min). The column was maintained at 22°C and the injection volume was 5 µL.
The UHPLC system was coupled to a hybrid quadrupole time-of-flight (QTOF) mass spectrometer (Agilent 6540 Series Accurate Mass QTOF-MS) with Dual ESI interface and operated in positive ion mode. Operation conditions were as follow: sheath gas temperature 400°C at flow rate of 12 L min⁻¹, capillary voltage 4000 V, nebulizer pressure 20 psig, drying gas 10 L min⁻¹, gas temperature 325°C, skimmer voltage 45 V, octopole RF peak 750 V and fragmentor voltage 100 V. Analysis were performed using MS/MS or TargetMS/MS mode with variable collision energies (10, 20 or 30 V; however, 20 V was the optimal value for all the target compounds) and in mass range 50–1000 m/z. The instrument was operated in the 4 GHz high-resolution mode and acquisition rate was 1.5 spectra per second. Acquisition data were processed with Agilent MassHunter Workstation software.

Jakimska A., Śliwka-Kaszyńska M., Nagórski P., Namieśnik J, & Kot-Wasik A. (2014). Phototransformation of Amlodipine: Degradation Kinetics and Identification of Its Photoproducts. PLoS ONE, 9(10), e109206.

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Purification and Characterization of 9-Methoxyluminarine

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9-Methoxyluminarine, 9-MeLM was obtained as a gift sample from Prof. B. Skalski, Faculty of Chemistry, Adam Mickiewicz University, Poznań. The purity of the ligand was examined using the HPLC technique. The analysis was performed on an HPLC Agilent 1260 Infinity system, on X Terra MS C18 Column, (3.5 µm, 4.6 × 250 mm) at 25 °C, eluted with H20, using a linear gradient of 0–100% of acetonitrile over 15 min at a flow rate of 0.8 mL/min. The ligand sample was dissolved in H₂O to obtain a stock solution of 1.3 mM concentration, which was stored at 4 °C.

Nowak-Karnowska J., Głuszyńska A., Kosman J., Neunert G, & Dembska A. (2021). Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies. International Journal of Molecular Sciences, 22(19), 10399.

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