Benzo h quinoline

Rhodium(III) trichloride hydrate was purchased from Precious Metals Online (PMO Pty Ltd.) and used as received. [Cp*Rh(μ-Cl)Cl]₂, benzo[h]quinoline, silver nitrate, sodium formate, sodium pyruvate, β-nicotinamide adenine dinucleotide reduced disodium salt (NADH), and celite were purchased from Sigma-Aldrich. MeOD-d₄, D₂O, and CDCl₃-d₁ for NMR spectroscopy and Quantofix® peroxides test sticks (1-100 mg/L range) were purchased from Sigma-Aldrich. Reduced glutathione was obtained from Alfa Aesar. Disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate, anhydrous sodium acetate, HPLC grade solvents (water and acetonitrile) with added trifluoroacetic acid with analytic grade, anhydrous DCM/pyridine and laboratory grade solvents used in syntheses were purchased from fisher scientific. A549 human lung and A2780 human ovarian cancer cell lines were purchased from the European Collection of Animal Cell Culture (ECACC, Salisbury, U.K.). Roswell Park Memorial Institute (RPMI-1640) medium, and phosphate-buffered saline (PBS) were purchased from PAA Laboratories GmbH. The total ROS/superoxide detection kit was purchased from Enzo Life Sciences. The apoptosis detection regents were purchased from Abcam.

Benzo[h]quinoline and iodopentoxide, were purchased from Sigma-Aldrich. The formylthiophenes 2-thiophenecarboxaldehyde, 2,2′-bithiophene-5-carboxaldehyde, and 5-[5-(2-thienyl)-2-thienyl]thiophene-2-carbaldehyde were purchased from Maybridge. Ethylene diamine was purchased from Alfa Aesar, and benzaldehyde was purchased from ACP. All of these chemicals were used without further purification. [Ru(bpy)₂Cl₂]·2H₂O was prepared as previously described.¹⁷ The cyclometalating ligands IBQ-nT, where n=1–4, were synthesized from benzo[h]quinoline-5,6-dione¹⁸ and the corresponding formylthiophenes by a method previously reported by us.^{14 (link)} Formyltetrathiophene was synthesized following a published method,^{19 ,20} with full characterization details reported previously by us.^{14 (link)} We are not aware of any unexpected, new, and/or significant hazards or risks associated with the reported work. However, utmost precaution should be taken when working with novel compounds of unknown risk.