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Flag m2

Manufactured by Abcam
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Sourced in United Kingdom

The FLAG M2 is a monoclonal antibody that recognizes the FLAG epitope tag. It is commonly used for the detection and purification of recombinant proteins expressed with a FLAG tag.

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Lab products found in correlation

Chd4 a301 081a, by Fortis Life Sciences (1 mentions) Hrp anti mouse, by Jackson ImmunoResearch (1 mentions)

2 protocols using flag m2

1

Antibody Validation for Protein Analysis

Cited 1 time
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The following antibodies were used: α-Tubulin (T9026); Acetyl-Histone H4 (06–866 lot#2554112, EMD Millipore (Billerica, MA)); CHD4 (A301-081A, Bethyl Laboratories (Montgomery, TX)); FITC anti-mouse (715-095-150 lot#115855, Jackson ImmunoResearch (West Grove, PA)); FLAG M2 (F1804 lot#SLBG5673V and lot#124K6106); FLAG M2 (F3165 lot#SLBL1237V); HDAC2 (ab7029, lot#GR88809-7, Abcam (Cambridge, UK)); HRP anti-mouse (115-035-146, Jackson ImmunoResearch); MBD2 (A301-632A, Bethyl); mouse IgG (315-005-003 lot#120058, Jackson ImmunoResearch); MTA2 (ab8106 lot#GR185489-3, Abcam); TRITC anti-rabbit (711-025-152 lot#114768, Jackson ImmunoResearch); rabbit monoclonal antibodies against DUX4 (E5-5 and E14-3) were produced in collaboration with Epitomics and are described elsewhere (Geng et al., 2012 (link)).
Campbell A.E., Shadle S.C., Jagannathan S., Lim J.W., Resnick R., Tawil R., van der Maarel S.M, & Tapscott S.J. (2018). NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins. eLife, 7, e31023.
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2

Antibody Reagents for Protein Analysis

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Antibodies were from Enzo Life Science (ubiquitin FK2), Sigma (Flag M2), Abcam (WIPI2, ab101985; TBK1, ab40676), Dabco (HRP‐conjugated reagents), and Invitrogen (Alexa‐conjugated anti‐mouse and anti‐goat antisera).
Thurston T.L., Boyle K.B., Allen M., Ravenhill B.J., Karpiyevich M., Bloor S., Kaul A., Noad J., Foeglein A., Matthews S.A., Komander D., Bycroft M, & Randow F. (2016). Recruitment of TBK1 to cytosol‐invading Salmonella induces WIPI2‐dependent antibacterial autophagy. The EMBO Journal, 35(16), 1779-1792.
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