Immunoprecipitation was performed using the following antibodies: IMPDH2 (Abcam, ab129165), RAC1 (Santa Cruz Biotechnology, sc-514583), Pierce™ Anti-c-Myc Magnetic Beads (Thermo Fisher Scientific, 88842). Immunoblotting was performed with the following antibodies: RAC1 (Proteintech, 24072-1-AP 1:500), GMPS (Abcam, ab228716 1:1000), IMPDH2 (Proteintech, 67663-1 1:500), Tubulin (Proteintech, HRP-66031 1:1000), GMPR (Proteintech, 15683-1-AP 1:1000), NME1 (Proteintech, 11086-2-AP 1:1000), ITGB1 (Proteintech, 26918-1-AP 1:1000), KLF9 ((Santa Cruz Biotechnology, sc-376422 1:250), RHOA (Cell Signaling, #2117, 1:500), CDC42 (Cell Signaling, #8747, 1:200), and RAS (Cell Signaling, #8832, 1:500).
Impdh2
Manufactured by Proteintech
Sourced in United States
IMPDH2 is an enzyme involved in the biosynthesis of guanine nucleotides. It catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP), which is a key step in the de novo purine biosynthetic pathway.
Automatically generated - may contain errors
Lab products found in correlation
Tubulin, by Proteintech (1 mentions)
Impdh2, by Abcam (1 mentions)
Cdc42, by Cell Signaling Technology (1 mentions)
Pierce anti c myc magnetic beads, by Thermo Fisher Scientific (1 mentions)
Itgb1, by Proteintech (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Boster Bio (1 mentions)
Ripa buffer, by Boster Bio (1 mentions)
P erk1 2, by Cell Signaling Technology (1 mentions)
Mek1 2, by Proteintech (1 mentions)
Fanci, by Santa Cruz Biotechnology (1 mentions)
Phospho mek1 2, by Cell Signaling Technology (1 mentions)
Erk1 2, by Proteintech (1 mentions)
Gapdh, by Proteintech (1 mentions)
2 protocols using impdh2
1
Immunoprecipitation and Immunoblotting Assays
2
Protein Extraction and Western Blot Analysis
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Total protein was extracted from cells using RIPA buffer (Boster, Wuhan, China) containing the protease inhibitor PMSF (Boster). Proteins were resolved by SDS-PAGE and transferred to PVDF membranes. The blots were blocked by incubation with 5% fat-free milk at room temperature for 2 h and then incubated overnight at 4°C with a 1:500 dilution of antibodies to the following proteins: FANCI (Santa Cruz Biotechnology, Dallas, TX, USA), IMPDH2, MEK1/2, ERK1/2, MMP2, MMP9, GAPDH (all Proteintech, Wuhan, China), phospho (p)-MEK1/2, and p-ERK1/2 (both Cell Signaling Technology, Danvers, MA, USA). The membranes were washed three times with TBST and then incubated for 2 h with horseradish peroxidase-conjugated rabbit or mouse secondary antibodies. After signal development, expression of proteins was analyzed using ImageJ software (National Institutes of Health, Bethesda, MD, USA).
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