Genistin 6 o acetate

Daidzein, daidzin, daidzin 6″-O-acetate, daidzin 6″-O-malonate, genistein, genistin, genistin 6″-O-acetate, genistin 6″-O-malonate, glycitein, glycitin, glycitin 6″-O-acetate, and glycitin 6″-O-malonate were purchased from FUJIFILM Wako Pure Chemical Corp (Osaka, Japan). CMS; dimethyl sulfoxide (DMSO); Gelrite™; indole-3-butyric acid (IBA); ascorbic acid; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; methyl jasmonate (MJ); and phosphate-buffered saline were purchased from Sigma-Aldrich Co., LLC (St. Louis, MO, USA). Mass-grade formic acid (FA), acetonitrile, methanol, and water were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The other chemicals employed in this study were of American Chemical Society grade or higher. The Sinhwakong soybean variety (an elite cultivar) was supplied by the National Institute of Crop Science (Cheonju, Republic of Korea).

Apigenin, Apigenin 7-O-glucoside, daidzein, daidzin, daidzin 6″-O-acetate, daidzin 6″-O-malonate, genistein, genistin, genistin 6″-O-acetate, genistin 6″-O-malonate, glycitein, glycitin, glycitin 6″-O-acetate, and glycitin 6″-O-malonate were purchased from Fujifilm Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Coumestrol, dimethyl sulfoxide (DMSO), kaempferol, kaempferol 3-O-glucoside (astragalin), isoquercitrin, isorhamnetin, luteolin, quercetin, and rutin were purchased from Sigma-Aldrich Co., LLC (St. Louis, MO, USA). The purities of the authentic compounds are listed in Table S1. Two quercetin diglycosides, two quercetin triglycosides, two kaempferol diglycosides, and three kaempferol triglycosides were purified from SLs. Mass-grade formic acid, acetonitrile, methanol, and water were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Other chemicals used were of American Chemical Society grade or higher.