Log In Sign up
The largest database of trusted experimental protocols

Teg 5000

Manufactured by Haemonetics
Visit Supplier
Sourced in United States

The TEG 5000 is a laboratory instrument used to analyze the viscoelastic properties of whole blood samples. It provides quantitative measurements of the clotting process, including the rate of clot formation and the strength of the clot. The device is designed to assist healthcare professionals in assessing the coagulation status of patients.

Automatically generated - may contain errors

Lab products found in correlation

Teg 6, by Haemonetics (1 mentions) Kaolin, by Haemonetics (1 mentions) Xe 2100, by Sysmex (1 mentions) Teg 5000 analyzer, by Haemonetics (1 mentions) Kaolin tube, by Haemonetics (1 mentions) 0.2 m calcium chloride, by Haemonetics (1 mentions) Thrombelastograph hemostasis analyzer system, by Haemonetics (1 mentions) Rhodamine 6g, by Merck Group (1 mentions) Ferric chloride, by Merck Group (1 mentions) Tiplaxtinin, by Axon Medchem (1 mentions) Actilyse, by Boehringer Ingelheim (1 mentions) Collagen, by Thermo Fisher Scientific (1 mentions) Fibrinogen, by Merck Group (1 mentions) Thrombin, by Merck Group (1 mentions) Cell lab quanta sc, by Beckman Coulter (1 mentions) Cd61 fitc, by Thermo Fisher Scientific (1 mentions) Pfa 100, by Siemens (1 mentions) Aggram, by Helena Laboratories (1 mentions)

27 protocols using teg 5000

1

Thromboelastography and Platelet Mapping

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Thromboelastography with platelet mapping was determined using a TEG 5000 (Haemonetics Corp., Braintree, Mass) using the intrinsic pathway activator kaolin (Haemonetics Corp.) and recalcification to 10 mmol/L final calcium concentration. The TEG 5000 reported time to onset of clot formation (R), which positively correlates with thrombin generation; the time to reach a predetermined level of clot stiffness (K) and the clotting angle (α-angle), which correlates with fibrin polymerization; the maximal amplitude (MA) or stiffness, representing clot strength. Platelet mapping was done using the TEG-5000 instrument and a platelet mapping kit (Haemonetics Corp.) that tests the platelet component to clot formation (MA) on TEG [21 (link)]. Briefly, heparinized blood treated with reptilase and activated factor XIII was used to form thrombin independent cross-linked fibrin. The platelet specific component for the adenosine diphosphate (ADP) and thromboxane receptor pathways were determined by activation with ADP and arachidonic acid (AA), respectively, on the heparinized blood samples. All assays were performed according to manufacturer guidelines.
Liebrecht L.K., Newton J., Martin E.J., Wickramaratne N., Jayaraman S., Han J., Aboutanos M., Brophy D.F, & Mangino M.J. (2019). Effects of a novel low volume resuscitation solutions on coagulation and platelet function. PLoS ONE, 14(5), e0215386.
+ Open protocol
+ Expand
2

Baseline Coagulation Assessment Pre- and Post-CPB

Check if the same lab product or an alternative is used in the 5 most similar protocols
Baseline investigations were performed following induction of anaesthesia, but prior to systemic heparinisation. These were the following: kaolin ACT, kaolin and heparinase TEG, full blood examination, prothrombin time (PT), and activated partial thromboplastin time (aPTT). TEG parameters were measured using the TEG 5000 or TEG 6s devices (Haemonetics, Braintree, Massachusetts, USA). These investigations were repeated 3 minutes after protamine administration following separation from CPB to ensure complete circulation of protamine before sampling was performed.
Miles L.F., Burt C., Arrowsmith J., McKie M.A., Villar S.S., Govender P., Shaylor R., Tan Z., De Silva R, & Falter F. (2021). Optimal protamine dosing after cardiopulmonary bypass: The PRODOSE adaptive randomised controlled trial. PLoS Medicine, 18(6), e1003658.
+ Open protocol
+ Expand
3

Viscoelastic Analysis of Clot Formation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Viscoelastic properties of clot formation and lysis were measured using the TEG 5,000 (TEG, Haemonetics, Boston, Mass). The RapidTEG reagent was used to initiate clot formation according to the manufacturer's instructions. RapidTEG (rTEG) activates both the intrinsic and extrinsic clot formation pathways to provide a rapid assessment of clot formation, clot strength, and fibrinolysis. Measured parameters include activated clotting time (ACT), α-angle, maximum amplitude (MA), and clot lysis at 30 min (LY30). These parameters measure the speed of clot initiation, rate of clot development, maximum clot strength, and rate of fibrinolysis respectively. Reference ranges were defined by the manufacturer for α-angle (66°–82°), MA (54 mm–72 mm), and LY30 (0%–8%). An ACT value of >140 s was considered abnormal (25 (link)).
Keyloun J.W., Le T.D., Pusateri A.E., Ball R.L., Carney B.C., Orfeo T., Brummel-Ziedins K.E., Bravo M.C., McLawhorn M.M., Moffatt L.T, & Shupp J.W. (2020). Circulating Syndecan-1 and Tissue Factor Pathway Inhibitor, Biomarkers of Endothelial Dysfunction, Predict Mortality in Burn Patients. Shock (Augusta, Ga.), 56(2), 237-244.
+ Open protocol
+ Expand
4

Thrombelastography for Clot Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
The process of thrombus formation was quantified using thrombelastography at time points T0, T2, T4 and T8. After sampling of 1ml perfusate from the coronary sinus, 1μl protamine was added immediately before thromboelastography (TEG5000, Haemonetics Corporation, MA, USA) was started. The reaction time (R) required to initiate clot formation, kinetic time (K) measuring the speed of clot development, α-angle characterizing the kinetics of clot growth and the maximum amplitude (MA) describing clot strength were all calculated.
Abicht J.M., Kourtzelis I., Reichart B., Koutsogiannaki S., Primikyri A., Lambris J.D., Chavakis T., Holdt L., Kind A., Guethoff S, & Mayr T. (2016). Complement C3 inhibitor Cp40 attenuates xenoreactions in pig hearts perfused with human blood. Xenotransplantation, 24(1), 10.1111/xen.12262.
+ Open protocol
+ Expand
5

Evaluating Viscoelastic Properties of Proteome Mixtures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Viscoelastic measurements were evaluated using the TEG 5000 (Haemonetics, Braintree, MA, USA). Proteome mixtures were constructed as above, except fibrinogen was also added. Mixtures were diluted 50% into buffer solutions in TEG cups as above, generating solutions of pH 7.4 and 7.0 at 37 °C.
Gissel M., Brummel-Ziedins K.E., Butenas S., Pusateri A.E., Mann K.G, & Orfeo T. (2016). Effects of an acidic environment on coagulation dynamics. Journal of thrombosis and haemostasis : JTH, 14(10).
+ Open protocol
+ Expand
6

Thromboelastography for Coagulation Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thromboelastography (TEG) was performed using TEG 5000 equipment according to the manufacturer's description (Haemonetics Corporation, Braintree, MA, USA). For whole blood thromboelastography, citrated blood samples were spiked with FXa or thrombin inhibitor, clotting factor concentrate (PCC, FVC), or solvent. Other additives were TF and tPA, which were added just before the start of the measurement to a final concentration of 1 pM and 200 ng/ml, respectively. Additives accounted for 9% v/v of the final blood sample. A prepared sample (340 µl) was transferred into a TEG cup, and measurements were started directly after adding 20 µl of 0.2 mM CaCl2. Derived parameters were reaction time R (equivalent to CT), maximal amplitude (MA), and CLT (elapsed time between MA and 2‐mm amplitude post‐MA).42 Used concentrations of TF and tPA allowed completion of the test within 90 min under all experimental conditions.
Brinkman H.J., Swieringa F., Zuurveld M., Veninga A., Brouns S.L., Heemskerk J.W, & Meijers J.C. (2022). Reversing direct factor Xa or thrombin inhibitors: Factor V addition to prothrombin complex concentrate is beneficial in vitro. Research and Practice in Thrombosis and Haemostasis, 6(3), e12699.
+ Open protocol
+ Expand
7

Venous Blood Coagulation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Each venous blood sample was collected into an citrate-containing vacuum blood collection tube (Becton Dickinson Medical Devices Co. Ltd., NC, USA). Professional technical personnel in our laboratory department analyzed the specimens within 2 h. All samples were kept at room temperature. Samples were gently mixed by inverting the tube five to ten times and were then run simultaneously on a two-channel TEG analyzer (TEG® 5000, Haemonetics Corp., Niles, IL, USA). Into each cup, 20 microliters of calcium chloride (CaCl2) and 340 microliters of samples were pipetted. Analysis was started immediately. The TEG values reaction time (R), K-time (K), angle, maximum amplitude (MA), and coagulation index (CI) were recorded. CBC data were processed by Sysmex XE-2100 (Sysmex Corporation, Kobe, Japan). Three levels of internal quality control (IQC) were made every day. The results of external quality assessment (EQA) were qualified.
Zhang M., He K., Ye D., Zhang Q, & Zhang Z. (2022). To Investigate Whether Hematocrit Affects Thromboelastography Parameters. Contrast Media & Molecular Imaging, 2022, 8877321.
+ Open protocol
+ Expand
8

Thromboelastography Assay for Coagulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The TEG was performed on previously described citrated whole blood samples within 2 hr after blood collection by using a TEG Analyzer 5000 (Haemonetics Corp., Braintree, MA, USA) based on the manufacturer's instructions. Briefly, 20 µL of 0.2M calcium chloride, 20 µL of diluted human tissue factor (TF), and 340 µL of the blood sample, were added to the TEG cup. The samples were allowed to react for 90 min, and the clot formation and lysis processes were recorded as a curve. Several parameters were generated: reaction time (R, min), which represented the time from the start of the test to the initial clot formation; coagulation time (K, min), which denoted the time for clot formation to reach a 20 mm amplitude; angle (α, degree), which indicated the speed of fibrin buildup; maximum amplitude (MA), which referred to the maximum strength of the fibrin clot formation; and LY30, which represented the percentage lysis after 30 min post-MA. The R, K, α, and MA were measured by using the TEG 5000 software (Haemonetics Corp.) and incorporated into a coagulation index (CI), which was calculated by using the following equation: CI=−0.6516R−0.3772K+0.1224MA +0.0759α−7.7922.
Kim S.Y., Gu J.Y., Yoo H.J., Kim J.E., Jang S., Choe S., Koh Y., Kim I, & Kim H.K. (2017). Benefits of Thromboelastography and Thrombin Generation Assay for Bleeding Prediction in Patients With Thrombocytopenia or Hematologic Malignancies. Annals of Laboratory Medicine, 37(6), 484-493.
+ Open protocol
+ Expand
9

Thromboelastography in Migraine Headache

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thromboelastography was performed on whole blood and on platelet poor plasma (obtained by centrifugation of citrated native blood and thawed after storage at −80°C) at baseline and during the headache phase of migraine using a TEG 5000 computer-controlled device (Haemonetics Corp., Niles, IL, USA) and following manufacturer's instructions. This method was used to monitor the viscoelastic profiles of migraineurs at baseline and during the headache phase of migraine.
de Villiers S., Bester J., Kell D.B, & Pretorius E. (2019). A Possible Role of Amyloidogenic Blood Clotting in the Evolving Haemodynamics of Female Migraine-With-Aura: Results From a Pilot Study. Frontiers in Neurology, 10, 1262.
+ Open protocol
+ Expand
10

Coagulation Profiling of a Novel Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols
Coagulation profiles were collected using a thromboelastograph (TEG 5000, Haemonetics). The instrument was calibrated with citrated platelet rich bovine plasma containing tissue factor (Factor III) with two levels of clot strength (Level I and II Control Plasma, Haemonetics). Test samples (n = 3) were prepared by mixing 4 mg of NMF with 340 ml of Level I control plasma. Coagulation profiles were recorded after adding 20 ml of 0.2 M CaCl2. Specific parameters representing the three phases of the cell-based model of hemostasis (R, k, a angle, MA, G, and coagulation index [CI]) were collected. R is the reaction time for formation of the first levels of detectable clot formation and represents the enzymatic portion of coagulation. K is the time to reach a fixed level of clot strength (amplitude of 22 mm) and represents initial clot kinetics. The angle a measures the rapidity of fibrin buildup and cross linking as the clot strengthens and represents fibrinogen level. Maximum amplitude (MA) is a direct function of clot strength and is reflective of platelet function. G is the shear elastic modulus strength and expresses clot firmness while the CI is an overall assessment of coagulability.22 Arista was used as positive control, Level I plasma after addition of calcium chloride was used as a standard.
Denry I., Nédélec J.M, & Holloway J.A. (2021). Tranexamic acid-loaded hemostatic nanoclay microsphere frameworks. Journal of biomedical materials research. Part B, Applied biomaterials, 110(2), 422-430.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!