The number of viable yeasts in the infected organs (lung, liver, and spleen) was determined by counting the number of colony-forming units (CFU), as previously described (36 (link)). Survival studies were carried out with groups of 10 to 12 mice. Deaths were recorded daily. For histological examinations, the left lung of infected mice was removed and fixed in 10% formalin. Five-micrometer sections were stained with HE for lesion analysis and silver (Grocott) for fungal evaluation. Morphometric analysis was performed using a Nikon DXM 1200c camera and Nikon NIS AR 2.30 software.
Nis ar 2
Manufactured by Nikon
The NIS AR 2.30 is a software solution that provides advanced image analysis capabilities for microscopy applications. It offers a comprehensive set of tools for image acquisition, processing, and analysis, enabling users to extract quantitative data from their microscopy samples.
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Lab products found in correlation
4 protocols using nis ar 2
1
Quantifying Fungal Infection in Mice
2
Quantifying Fungal Lung and Liver Infection
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The number of viable yeasts in lung and liver was determined by counting the number of colony-forming units (CFU) as previously described71 (link). Mortality studies were done with groups of 10–12 mice. Deaths were registered daily. For histological examinations, five-micrometer tissue sections were stained by hematoxylin–eosin for characterization of lesions and were silver stained (Grocott stain) for fungal evaluation. Morphometrical analysis was performed using a Nikon DXM 1200c camera and Nikon NIS AR 2.30 software. The areas of lesions were measured (in square micrometers) in 10 microscopic fields per slide in 5 mice per group as previously described72 (link). Results are expressed as the mean ± SEM of total area of lesions for each mouse.
3
Quantifying Fungal Infection Dynamics
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The numbers of viable microorganisms in cell cultures and infected organs were determined by counting the number of colony-forming units (CFU) as previously described [69 ]. Mortality studies were done with groups of 10–12 mice. Deaths were registered daily. For histological examinations, the left lung and liver of infected mice was removed and fixed in 10% formalin. Five-micrometer sections were stained by hematoxylin-eosin for an analysis of the lesions and were silver stained (Grocott stain) for fungal evaluation. Morphometrical analysis was performed using a Nikon DXM 1200c camera and Nikon NIS AR 2.30 software. The areas of lesions were measured (in square micrometers) in 10 microscopic fields per slide in 5 mice per group. Results are expressed as the mean ± SEM of total area of lesions for each mouse.
4
Fungal Burden and Histological Analysis
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The number of viable yeast cells in the lungs, liver, and spleen was determined by counting the number of CFU, as previously described (49 (link)). Mortality studies were performed using groups of 10–11 mice. Deaths were registered daily. For histological examinations, 5-μm-thick tissue sections were stained with hematoxylin-eosin (HE) and silver (Grocott stain) for lesions and fungal evaluation, respectively. Morphometric analysis was performed using a Nikon DXM 1200c camera and Nikon NIS AR 2.30 software. The areas of lesions were measured (in μm2) in 10 microscopic fields per slide in 5 mice per group, as previously described (50 (link)). Results are expressed as the mean ± SEM of the total lesion area for each mouse.
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