Western blotting was performed following the method we used in our previous study [13 ]. The antibodies included anti-rabbit BMP2 (1:2000, BOSTER, China), anti-rabbit OPN (1:2000, SANTA CRUZ, USA), anti-rabbit OCN (1:2000, SANTA CRUZ, USA), anti-rabbit β-actin antibodies (1:2000, BOSTER, China), anti-mouse TSG101 (1:2000, Abcam, USA), anti-mouse CD9 (1:2000, Abcam, USA), anti-mouse Alix (1:2000, Abcam, USA),anti-mouse Calnexin (1:2000, Abcam, USA), and anti-rabbit and mouse immunoglobulin G (IgG) horseradish peroxidase (HRP)-linked antibody (1:5000, BOSTER, China). All WB quantification was performed using three independent WBs.
Anti rabbit opn
Manufactured by Santa Cruz Biotechnology
Sourced in China, United States
Anti-rabbit OPN is a laboratory reagent used for the detection and quantification of osteopontin (OPN) in rabbit samples. It is a specific antibody that binds to rabbit OPN, allowing for its identification and measurement in various experimental and diagnostic applications.
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Lab products found in correlation
2 protocols using anti rabbit opn
1
Protein Expression Analysis by Western Blot
2
Western Blot Analysis of Myogenic Markers
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Western blotting was performed as previously described [54 (link)]. After incubation with the primary antibodies, including anti-rabbit Myocardin (Sigma, United States), anti-rabbit MRTF-A (Abcam, United States), anti-rabbit SRF (Santa Cruz, United States), anti-rabbit ACTA2 (Abcam, United States), anti-rabbit SM-22 (Abcam, United States), anti-rabbit OPN (Santa Cruz, United States), anti-rabbit CyclinD1 (Santa Cruz, United States), anti-Rabbit CYR61 (Santa Cruz, United States), anti-rabbit MYL9 (Santa Cruz, United States), anti-rabbit LC3 (Novus Biologicals, United States), anti-mouse P62 (BD Transduction Laboratories, United States), anti-rabbit ATG7 (Bioss, China) and anti-mouse β-actin (Santa Cruz, United States) antibodies; the membranes were incubated with the appropriate secondary antibodies, including IR Dye-800 conjugated anti-rabbit IgG secondary antibody and IR Dye-680 conjugated anti-mouse IgG. The specific protein was visualized using the Odyssey Infrared Imaging System (Gene Company, HongKong). β-actin expression was used as an internal control to show equal loading of the protein sample. The relative quantity of proteins was analyzed using Image J software. The relative quantity of proteins was analyzed using Image J software.
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