Caspase 3 assay kit

Manufactured by Qiagen

The Caspase 3 Assay kit is a laboratory tool used to detect and measure the activity of the Caspase 3 enzyme. Caspase 3 is a key regulator of apoptosis, a form of programmed cell death. The assay kit provides a quantitative method for evaluating Caspase 3 levels in cell and tissue samples.

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Lab products found in correlation

Gapdh primer, by Qiagen (2 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions) Penicillin streptomycin antibiotic, by Thermo Fisher Scientific (2 mentions) Annexin 5 fitc apoptosis detection kit, by Merck Group (2 mentions) Mitotracker green, by Merck Group (2 mentions) Bcl 2, by Qiagen (2 mentions) Hek 293, by Iranian Biological Resource Center (2 mentions) Bcl2 associated x (bax), by Qiagen (2 mentions) 2 7 dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions)

2 protocols using caspase 3 assay kit

Cytotoxicity of Anti-Cancer Agents

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Human colorectal adenocarcinoma (HT-29) and human embryonic kidney 293 (HEK-293) cell lines were purchased from Iranian Biological Resource Center (Tehran, Iran). The anti-cancer agents, 5-FU and DCA/3Br-P, were supplied by Ebewe Pharma company and Sigma Aldrich (Saint Louis, MO), respectively. Dulbecco’s modified eagle’s medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), and antibiotic (penicillin-streptomycin) were purchased from Invitrogen Co, (Carlsbad, CA). Furthermore, MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide), 2,7-dichlorofluorescein diacetate (DCFH-DA), Annexin V/FITC apoptosis detection kit and MitoTracker Green supplied by Sigma Aldrich (Saint Louis, MO) were applied in this work. Moreover, Caspase 3 Assay kit, Bax, Bcl-2, and GAPDH primers were obtained from Qiagen (Hilden, Germany).

Nikravesh H., Khodayar M.J., Behmanesh B., Mahdavinia M., Teimoori A., Alboghobeish S, & Zeidooni L. (2021). The combined effect of dichloroacetate and 3-bromopyruvate on glucose metabolism in colorectal cancer cell line, HT-29; the mitochondrial pathway apoptosis. BMC Cancer, 21, 903.

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Anticancer effects of 5-FU and DCA/3Br-P

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Human colorectal adenocarcinoma (HT-29) and human embryonic kidney 293 (HEK-293) cell lines were purchased from Iranian Biological Resource Center (Tehran, Iran). The anti-cancer agents, 5-FU and DCA/3Br-P, were supplied by Ebewe Pharma company and Sigma Aldrich (Saint Louis, MO), respectively. Dulbecco's modi ed eagle's medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), and antibiotic (penicillin-streptomycin) were purchased from Invitrogen Co, (Carlsbad, CA). Furthermore, MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide), 2,7-dichloro uorescein diacetate (DCFH-DA), Annexin V/FITC apoptosis detection kit and MitoTracker Green supplied by Sigma Aldrich (Saint Louis, MO) were applied in this work. Moreover, Caspase 3 Assay kit, Bax, Bcl-2, and GAPDH primers were obtained from Qiagen (Hilden, Germany).

Nikravesh H., Khodayar M.J., Behmanesh B., Mahdavinia M., Teimoori A., Alboghobeish S., & Zeidooni L. (2021). The Combined Effect of Dichloroacetate and 3-Bromopyruvate in Colorectal Cancer Cell Line, HT-29; The Mitochondrial Pathway Apoptosis.

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