Lipopolysaccharides (LPS; L2880) and the anti-glial fibrillary acidic protein (anti-GFAP; G9269) antibody were purchased from Sigma (St. Louis, MO, United States). The complement C3 polyclonal antibody (sc-58926) was purchased from Santa Cruz Biotechnology (Dallas, TX, United States). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), and penicillin-streptomycin, 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Life Technologies (Carlsbad, CA, United States). Anti-tubulin antibodies (2148S) were purchased from Cell Signaling Technology (Beverly, MA, United States). Goat anti-rabbit IgG H&L (Alexa Fluor 488), rabbit anti-rat IgG H&L (Alexa Fluor 555), TNF-α enzyme-linked immunosorbent assay (ELISA) kits, and IL-1α ELISA kits were purchased from Abcam (San Francisco, CA, United States). C1q ELISA Kit (mouse; HK211) was purchased from Hycult Biotech (Plymouth Meeting, PA, United States). The anti-aquaporin 4 (AQP4) (249-323) antibody (AQP-004) was purchased from Alomone Labs (Jerusalem, Israel). The TREM2 polyclonal antibody (PA5-87933) and lactate dehydrogenase (LDH) release were purchased from Thermo Fisher Scientific (Waltham, MA, United States). Cell Counting Kit-8 was purchased from Dojindo Molecular Technologies (Rockville, MD, United States).
Penicillin streptomycin 4 6 diamidino 2 phenylindole dapi
Manufactured by Thermo Fisher Scientific
Sourced in United States
Penicillin-streptomycin is a common antibiotic solution used in cell culture applications to prevent bacterial contamination. 4′,6-diamidino-2-phenylindole (DAPI) is a fluorescent stain that binds to DNA and is used in microscopy techniques to visualize nuclei.
Automatically generated - may contain errors
2 protocols using penicillin streptomycin 4 6 diamidino 2 phenylindole dapi
1
Neuroinflammation and Astrocyte Activation
2
Investigating Cell Signaling Pathways with LPS and siRNA
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Lipopolysaccharides (LPS; L2880) and X-tremeGENE™ siRNA transfection reagent (4476093001) were purchased from sigma (St. Louis, MO). Dulbecco's modi ed Eagle's medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), and penicillin-streptomycin, 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Life Technologies (Carlsbad, CA). Anti-Tubulin antibodies (2148S), anti-GAPDH(2118S), anti-YAP(14074T), anti-β-Actin antibody(4967S), anti-TBP antibody(8515S), anti-TAZ(83669S), anti-p-YAP (Ser127) (13008T), anti-YAP/TAZ(93622), anti-MST1(14946S), anti-p-(Thr183)/MST2 (Thr180)(49332S), anti-VEGFR2(9698S), Anti-rat IgG (Alexa Fluor® 488 Conjugate), Antirabbit IgG (Alexa Fluor® 555 Conjugate) were purchased from Cell Signaling Technology (Beverly, MA).anti-CD31 antibody(ab7388), anti-claudin-5(ab131259), were purchased from Abcam (San Francisco, CA). Anti-ZO-1 antibody (40-2200), anti-TDP43 (c-terminal) antibody(12892-1-AP), anti-TDP43 antibody (PA5-17011), BrdU™ Assay Kit(Alexa Fluor™ 488)(A23210), EdU Cell Proliferation Kit for Imaging(Alexa Fluor™ 488)(C10337), Ki-67 antibody (69-5698-82), and MTT Cell Viability Assay (V13154) were purchased from ThermoFisher Scienti c (Waltham, MA). Cell Counting Kit-8 was purchased from Dojindo Molecular Technologies (Rockville, MD).
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