All experiments involving mice were approved by Washington University’s Institutional Animal Care and Use Committee. We used 2–5 month old C57BL/6J and Thbs1−/− (Jackson Laboratories Cat# 006141; RRID:IMSR_JAX:006141) mice from Jackson Laboratories. To identify CCR2+ monocyte-derived macrophages in mouse choroidal neovascular lesions, we used heterozygote Ccr2+/gfp mice available from Jackson Laboratories (Jackson Laboratories Cat# 027619; RRID:IMSR_JAX:027619). Mice were housed in a barrier facility with a 12:12 light/dark cycle with water and food provided ad libitum.
Thbs1
Manufactured by Jackson ImmunoResearch
Thbs1−/− is a genetically engineered mouse strain with a targeted deletion of the Thbs1 gene, which encodes the thrombospondin-1 protein. This mouse model is used in research to study the biological functions of thrombospondin-1 and its role in various physiological and pathological processes.
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2 protocols using thbs1
1
Mouse Choroidal Neovascularization Models
2
Genetically Modified Mouse Models
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Thbs1-deficient (Thbs1-/- (#006141)), LysM-Cre (#004781) and Rosa26-EYFP (#006148) mice were purchased from Jackson Laboratories. C57BL/6 mice, GFP transgenic mice (C57BL/6-Tg (CAG-EGFP)) mice were purchased from Japan SLC,Inc. Systemic CD47-deficient (CD47-/-) (CD47f/f;E2A-Cre) mice were generated by crossing CD47f/f mice with E2A-Cre mice49 (link). Thbs1f/f mice were generated as previously described39 (link). CD36-deficient (CD36-/-) mice were kindly provided by Dr. Freeman (Harvard Medical School). All mouse strains were generated in a C57BL/6 background and were born and maintained under pathogen-free conditions. These mice were maintained in 14 hr light/10 h dark cycle, and the housing temperature and humidity were maintained were 24 °C and 50%, respectively. Animal handling and experimental procedures conformed to institutional guidelines and were approved by the animal research committee of Kyoto University (Kyoto, Japan) and performed in accordance with Japanese government regulations. 8–10 weeks old male mice were used in all experiments.
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