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Standard 24 well boyden control and invasion chambers

Manufactured by BD

The Standard 24-well Boyden control and invasion chambers are laboratory equipment used to study cell migration and invasion. These chambers consist of an upper and a lower compartment separated by a porous membrane. Cells are seeded in the upper compartment, and their ability to migrate through the membrane or invade a matrix coated on the membrane can be measured.

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3 protocols using standard 24 well boyden control and invasion chambers

1

Boyden Chamber Cell Migration Assay

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Standard 24-well Boyden control and invasion chambers (BD Biosciences) were used to assess cell migration and invasion following manufacturer suggestions. Details are in the Supplemental Information.
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2

Boyden Chamber Cell Migration Assay

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Standard 24-well Boyden control and invasion chambers (BD Biosciences) were used to assess cell migration and invasion following manufacturer suggestions. Details are in the Supplemental Information.
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3

Cell Proliferation and Migration Assay

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We utilized CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) kit (# G3582) from Promega to examine cell proliferation (16). Standard 24-well Boyden control and invasion chambers (BD Biosciences) were used to assess cell migration and invasion following manufacturer suggestions. We cloned shRNAs into pGPU6/GFP/Neo vectors. The sequence of shRNA targeting hsa_circ_0001860-2 is "TTACTGAAGCTTCAAGGTTAC"; and the control shRNA sequence is "TTCTCCGAACGTGTCACGT".
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