To measure the ratio of the filled area in the reaction chamber, solution containing orange food dye was pumped into the microfluidic device using the miniaturized pumping system. The process of liquid filling was recorded with a camera (Canon 80D, EF 100 mm) and analyzed in ImageJ. The filling ratio was defined as the ratio of the filled area over the footprint of the reaction chamber.
Ef 100 mm
Manufactured by Canon
Sourced in Japan
The EF 100mm is a prime lens designed for Canon's EF mount cameras. It offers a fixed focal length of 100mm and a maximum aperture of f/2.8, providing a medium-telephoto field of view and good low-light performance. The lens features a high-quality optical design and is suitable for a variety of photographic applications.
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6 protocols using ef 100 mm
1
Microfluidic Filling Ratio Measurement
2
Microfluidic Vacuum-Driven Reagent Loading
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A microfluidic device was taken out from its vacuum package before measurement. Reagents were applied to their respective inlets using disposable droppers. The outlet hole was sealed through pressure and immediately the reagents started to be drawn towards the reagent reservoirs by the vacuum loaders. This loading process was recorded with a camera (Canon 80D, EF 100 mm) and analyzed with a video player (VLC). The loading time was determined from the instance of outlet sealing to the moment that the reagent reservoirs were fully filled.
3
Exoskeleton Compressive Strength Characterization
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Compression tests were performed using a ZwickiLine uniaxial compression testing machine (Zwick Roell, Ulm, Germany) equipped with a 500 N load cell (Xforce P load cell) on two mature and two teneral adults to measure the load-carrying capacity of the exoskeleton. After specimens were completely thawed at room temperature, they were placed in the center of a sample holder (102.34 × 89.79 mm). Two small pieces of rough sandpaper were glued at the surface of the sample holder and at the tip of the load cell (diameter: 14 mm) to avoid slippage of the specimens (Figure 1A ). The load cell compressed the specimens dorsally at a constantly increasing displacement of 1 mm/min. After reaching the maximal displacement of 4 mm followed by a three-second pause, the displacement was constantly decreased at a speed of 5 mm/min until complete unloading was achieved. The amount of the force and displacement were recorded using the test software testXpert (v. 3.5, Zwick Roell, Ulm, Germany). The testing process was recorded using a camera (D5300, Nikon Co., Tokyo, Japan) equipped with a macro lens (EF100 mm, Canon Inc., Tokyo, Japan).
4
Cross-polarized Microscopy of Specimens
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The digital images of the specimens were captured with a Canon EOS 5D SR camera mounted with Caron MP-E 65 mm (1–5×) or Canon EF 100-mm macro lenses under cross-polarised light, and were processed in Adobe Photoshop CC 2018. All measurements were processed with ImageJ version 1.49.
5
Camera-Based Stent Coil Diameter Measurement
6
Comprehensive Insect Specimen Curation and Analysis Protocol
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Studied specimens belong to the following collections:
BMNH British Museum of Natural History, London, UK MHBU
MNHN Muséum National d’Histoire Naturelle, Paris, France SNUC
SWU
SZPT School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen, Guangdong Province, China .
The terminology used mainly follows Costa et al. (2010) (link) and Douglas (2011) (link). The classification follows Cate et al. (2007) (link). Observations and measurements were made under a stereomicroscope Motic SMZ-168. Photographs were made using a digital microscope (LY-WN-YH 3D system), Canon EOS-1 camera with Canon EF 100 mm, 65 mm and 55–250 mm lens.
Measurements: body length was measured along the midline from the anterior edge of the head capsule to the apex of elytra; body width was measured across the broadest part (usually across the elytra). Pronotal length was measured along the midline; the pronotal width was measured across the broadest part (usually across the hind angles).
Specimens were mounted on paper cards. The genitalia were removed, cleaned and fixed under the body of the specimen in glycerol mounts following Prosvirov and Savitsky (2011) (link).
The terminology used mainly follows Costa et al. (2010) (link) and Douglas (2011) (link). The classification follows Cate et al. (2007) (link). Observations and measurements were made under a stereomicroscope Motic SMZ-168. Photographs were made using a digital microscope (LY-WN-YH 3D system), Canon EOS-1 camera with Canon EF 100 mm, 65 mm and 55–250 mm lens.
Measurements: body length was measured along the midline from the anterior edge of the head capsule to the apex of elytra; body width was measured across the broadest part (usually across the elytra). Pronotal length was measured along the midline; the pronotal width was measured across the broadest part (usually across the hind angles).
Specimens were mounted on paper cards. The genitalia were removed, cleaned and fixed under the body of the specimen in glycerol mounts following Prosvirov and Savitsky (2011) (link).
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