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Activity assay kits

Manufactured by Cayman Chemical
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Sourced in United States

Activity assay kits are laboratory reagents designed to measure the activity of specific enzymes or biological molecules. These kits provide a standardized and quantitative method to assess the functional capacity of the target analyte.

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Spelling variants of this product

COX Activity Assay Kit (34 citations) MitoCheck Citrate Synthase Activity Assay Kit (19 citations) NOS activity assay kit (8 citations) COX-2 Activity Assay kit (4 citations) Fluorescent activity assay kit (4 citations) CPLA2 activity assay kit (3 citations) Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (3 citations) MAO-A and LSD1 activity assay kits (2 citations) ALT and AST colorimetric activity assay kits (2 citations) Complex I activity assay kit (2 citations)

4 protocols using activity assay kits

1

Antioxidant Enzyme Activity Assay

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Serum levels of SOD, catalase and total glutathione were determined using commercially available activity assay kits purchased from Cayman Chemical (Ann Arbor, MI, USA). Samples were processed and assayed according to the manufacturer’s protocol.
Beharry K.D., Cai C.L., Henry M.M., Chowdhury S., Valencia G.B, & Aranda J.V. (2017). Co-Enzyme Q10 and n-3 Polyunsaturated Fatty Acid Supplementation Reverse Intermittent Hypoxia-Induced Growth Restriction and Improved Antioxidant Profiles in Neonatal Rats. Antioxidants, 6(4), 103.
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2

Antioxidant Enzyme Activity Assay

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Levels of SOD, catalase and total GSH, and total antioxidant capacity in the lung homogenates were determined using commercially available activity assay kits purchased from Cayman Chemical (Ann Arbor, MI, USA) and Millipore-Sigma (St. Louis, MO, USA), respectively. A total of 6 samples per experimental group were analyzed. Samples were processed and assayed according to the manufacturer’s protocol and standardized using total cellular protein levels.
D’Agrosa C., Cai C.L., Siddiqui F., Deslouches K., Wadowski S., Aranda J.V, & Beharry K.D. (2021). Comparison of coenzyme Q10 or fish oil for prevention of intermittent hypoxia-induced oxidative injury in neonatal rat lungs. Respiratory Research, 22, 196.
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3

Evaluating AOEs Activity Assays

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After rutin and TEGDMA treatment, the activity of AOEs, including superoxide dismutase (SOD) and catalase (CAT), was evaluated using activity assay kits (Cayman Chemical, Ann Arbor, MI, USA), according to the manufacturers’ protocols and previous studies [24 (link)].
Yang L.C., Chang Y.C., Yeh K.L., Huang F.M., Su N.Y, & Kuan Y.H. (2022). Protective Effect of Rutin on Triethylene Glycol Dimethacrylate-Induced Toxicity through the Inhibition of Caspase Activation and Reactive Oxygen Species Generation in Macrophages. International Journal of Molecular Sciences, 23(19), 11773.
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4

Antioxidant Enzyme Activity Assays

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Levels of SOD, catalase, and total GSH (n = 6 samples/group) were determined using commercially available activity assay kits (catalogue #s 706002, 707002, and 703002, respectively) purchased from Cayman Chemical (Ann Arbor, MI, USA). Samples were processed and assayed according to the manufacturer’s protocol and standardized using total cellular protein levels.
Siddiqui F., Cai C., Aranda J.V, & Beharry K.D. (2023). Coenzyme Q10 and Fish Oil Supplementation for Reducing Retinal Oxidative Stress in a Rat Model. Vision, 7(1), 20.
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