Uas ecr rnai

All flies were raised at 25°C under 12 hours/12 hours light/dark cycle. The animals were reared on standard fly food containing 6 g Drosophila agar type II (Genesee Scientific, #66–103), 100 g D-(+)-glucose (SIGMA, #G8270–25KG), 50 g inactive dry yeast (Genesee Scientific, #62–106), 70 g yellow corn meal (Genesee Scientific, #62–101), 6 ml propionic acid (SIGMA, #402907–500ML), and 10 ml Tegosept (Genesee Scientific, #20–258) in 1,025 ml of water. Flies used are as follows: w¹¹¹⁸ (#5905; the control strain), Mi{MIC}Oatp74D^MI05878 (#42353), EcRE-LacZ (#4516 and #4517), UAS-EcR RNAi (#9327), and UAS-mCD8-GFP (#5130) were obtained from the Bloomington Drosophila Stock Center (BDSC); UAS-dicer2 (#60008 and #60009) was obtained from the Vienna Drosophila Resource Center (VDRC); UAS-EcI (Oatp74D) RNAi (#7571R-1) was obtained from the National Institute of Genetics Fly Stock Center; NP2276-Gal4 (#112853) and NP6293-Gal4 (#105188) were obtained from the Kyoto Stock Center (Department of Drosophila Genomics and Genetic Resources, Kyoto Institute of Technology). 9–137-Gal4 and Moody-Gal4 were obtained from Ulrike Heberlein (Janelia Research Campus) and Ulrike Gaul (Ludwig-Maximilians-Universität München), respectively.