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Human et 1 quantiglo kit

Manufactured by R&D Systems

The Human ET-1 QuantiGlo kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure endothelin-1 (ET-1) levels in human cell culture supernates, serum, and plasma samples. The kit utilizes a luminescent QuantiGlo substrate to quantify the amount of ET-1 present in the sample.

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2 protocols using human et 1 quantiglo kit

1

Urinary Biomarkers in High Salt Diet

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ET-1 levels in undiluted urine samples were determined by a chemiluminescent assay (Human ET-1 QuantiGlo kit; R&D Systems). Urinary TNF-α concentration was determined by ELISA (R&D Systems), and H2O2 concentration was determined using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit (Thermo Fisher Scientific; Waltham, MA). Urinary concentration of Thiobarbituric Acid Reactive Substances (TBARS) was also assessed by ELISA (Cayman Chemical; Ann Harbor, MI). Excretion rates were determined from 24-hr urine volume collected in metabolic cages at the end of the 3 week period of on high salt diet.
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2

Measuring ET-1 in Plasma and Urine

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Levels of ET-1 in undiluted samples of plasma and urine were determined by a chemiluminescent assay (Human ET-1 QuantiGlo kit, R&D Systems, Minneapolis, MN).
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