Human m csf quantikine elisa kit

Manufactured by R&D Systems

The Human M-CSF Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human macrophage colony-stimulating factor (M-CSF) levels in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Human angiopoietin 2 quantikine elisa kit, by R&D Systems (1 mentions) Easysep mouse monocyte enrichment kit, by STEMCELL (1 mentions) Ack lysis buffer, by Quality Biological (1 mentions) Legendplex human m1 m2 macrophage panel, by BioLegend (1 mentions)

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Quantikine ELISA kit (1163 citations) M-CSF (827 citations) Quantikine ELISA (398 citations) Quantikine kits (80 citations) Human M-CSF (24 citations) Quantikine M (7 citations) Quantikines (4 citations) M-CSF ELISA (2 citations) Quantikine Human (2 citations) Human kits (2 citations)

3 protocols using human m csf quantikine elisa kit

Serum Ang-2 and CSF-1 Levels Quantification

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A 3 mL sample of venous blood was collected into serum separator tubes from each study participant before any treatment, allowed to coagulate at room temperature for 1 hour and then centrifuged before serum collection. Serums aliquots were stored at −20°C until analyses were carried on.
The measurement of CSF-1 serum concentration was performed through ELISA using the Human M-CSF Quantikine ELISA kit, purchased from R&D Systems (catalogue #DMC00B) and used the values of serum Ang-2 levels previously determined in this study population,24 (link) using the Human Angiopoietin-2 Quantikine ELISA kit, also from R&D Systems (catalogue #DANG20), according to manufacturer’s protocols. We performed the specimens’ assays in duplicate, using the average of the two measurements to data analysis. The minimum detectable levels of Ang-2 and CSF-1 were 1.2 pg/mL and 1.3 pg/mL, respectively.

Coelho A.L., Gomes M.P., Catarino R.J., Rolfo C., Medeiros R.M, & Araújo A.M. (2018). CSF-1 and Ang-2 serum levels — prognostic and diagnostic partners in non-small cell lung cancer. ESMO Open, 3(5), e000349.

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Murine Monocyte-Derived Macrophage Isolation

Cited 1 time

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Eight- to 10-week old male mice were used as cell donors. Mouse cMDM were prepared as described previously [5 (link)]. In brief, bone marrow cells (BMC) were isolated from donor mice femur and tibia of the hind limbs. Erythrocytes were removed by lysis with ACK lysis buffer (Quality Biological, Inc.), and a single cell suspension was obtained by passing through 40-μm cell strainers (BD Falcon, #352340), and was either resuspended in PBS and used for cell IV infusion, or cultured in suspension with complete growth medium composed of RPMI1640 medium supplemented with 10% FBS and 1,000 U/ml M-CSF (obtained from 5/9 m alpha3-18 cell media, ATCC#CRL-10154. M-CSF in condition media was quantified using Human M-CSF quantikine ELISA kit, R&D system cat#DMC00B), at 37°C with 5% CO₂ for 2, 5, 7, 9, 12, 15, 21 and 28 days prior to flow cytometry analysis or adoptive transfer study.
In studies using freshly isolated monocytes (Enriched monocytes, EnMo), monocytes were enriched from the freshly isolated BMC suspension using EasySep mouse monocyte enrichment kit (Stem Cell Technologies, #19761) according to manufacturer’s instructions.

Tong H.I., Kang W., Davy P.M., Shi Y., Sun S., Allsopp R.C, & Lu Y. (2016). Monocyte Trafficking, Engraftment, and Delivery of Nanoparticles and an Exogenous Gene into the Acutely Inflamed Brain Tissue – Evaluations on Monocyte-Based Delivery System for the Central Nervous System. PLoS ONE, 11(4), e0154022.

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Quantifying Macrophage Cytokine Profiles

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IL-34 and M-CSF titrations were performed with the Human IL-34 DuoSet ELISA and the Human M-CSF Quantikine ELISA kit (both from R&D Systems), respectively, following the manufacturers’ recommendations. The supernatants of MCTS were aliquoted and stored at −80°C until use. Cytokines were measured using the LEGENDplex Human M1/M2 macrophage panel (BioLegend) according to the manufacturer’s recommendations.

Blondy T., d'Almeida S.M., Briolay T., Tabiasco J., Meiller C., Chéné A.L., Cellerin L., Deshayes S., Delneste Y., Fonteneau J.F., Boisgerault N., Bennouna J., Grégoire M., Jean D, & Blanquart C. (2020). Involvement of the M-CSF/IL-34/CSF-1R pathway in malignant pleural mesothelioma. Journal for Immunotherapy of Cancer, 8(1), e000182.

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