HPLC analysis was performed as previously described (12 (link)). Specifically, analysis was performed using 95% water–5% acetonitrile–0.1% trifluoroacetic acid (TFA) as the mobile phase with a flow rate of 0.5 ml/min on a Microsorb-MV C18 column. After a 10-min equilibration period, a linear 40-min acetonitrile gradient (5% to 80%) was employed and the final concentration was maintained for an additional 20 min. The eluate was monitored using a photodiode array detector from 200 to 900 nm by reverse-phase chromatography on a Varian ProStar HPLC instrument.
Prostar hplc instrument
Manufactured by Agilent Technologies
Sourced in China
The ProStar HPLC instrument is a high-performance liquid chromatography system designed for analytical and preparative applications. It provides precise control and monitoring of mobile phase flow, column temperature, and detection parameters to facilitate reliable separation and analysis of a wide range of samples.
Automatically generated - may contain errors
3 protocols using prostar hplc instrument
1
HPLC Analysis of Compounds
2
Analytical Techniques for Natural Product Purification
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The following equipment was used: 3000-Da nanofiltration membrane (SJM, Hefei, China), AB-8 macroporous resin column (Bonc, Cangzhou, China), ordinary-phase silica gel column (200–300 mesh, Anhui Liangchen Silicon Material Co. Ltd., Huoshan, China) and a Varian Prostar HPLC instrument (Model 325) (Varian, Mulgrave, Australia). The HPLC purifications were run on Agilent 1260 HPLC (Agilent, Palo Alto, CA, USA). IR (infrared) spectra were recorded on Nicolet iN10 (Thermo Scientific Instrument Co., Boston, MA, USA) with KBr pellets. NMR spectra were measured on an AVANCE III (600 MHz) spectrometer (Bruker, Fallanden, Switzerland) using methanol-d4 as solvent g methanol-d4 (Sigma-Aldrich, St. Louis, MO, USA). HR-ESI-MS were determined on an Electrostatic Field Orbital Trap Mass Spectrometer (Thermo Scientific, Bremen, Germany) using an ESI source.
3
Polymer Molecular Weight Characterization
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GPC was used to estimate the average molecular weight of the polymers using a Varian ProStar HPLC instrument. All analyses were performed with a PL Gel 5 µm column. A special system was created for this analysis with a pressure of 4000 psi, injection volume of 20 µl and a flow rate of 1 ml/min. Each analysis was run for 35 minutes. The samples were injected into the column using a straight edged syringe and each sample was analysed thrice. A Varian ProStar ultravioletphotodiode array (UV-PDA) detector was used for the detection of the polymer at 245nm.
A calibration with polystyrene standards was performed using the same method and mobile phase.
A calibration plot of t R along the X-axis versus log M on the Y-axis was drawn for the polystyrene standards. The slope and intercept were calculated from the graph.
From the slope and intercept of the calibration curve, the number average (M n ), weight average molecular weight (M w ) and polydispersity (PD) were calculated using the following equations
Where N i is the number of moles with molecular weight M i ; N i and M i being determined from the following equations Log M i = slope × t R + intercept with slope = -0.38 and intercept = 9.92 this gives Log M i = -0.38 × t R + 9.92
A calibration with polystyrene standards was performed using the same method and mobile phase.
A calibration plot of t R along the X-axis versus log M on the Y-axis was drawn for the polystyrene standards. The slope and intercept were calculated from the graph.
From the slope and intercept of the calibration curve, the number average (M n ), weight average molecular weight (M w ) and polydispersity (PD) were calculated using the following equations
Where N i is the number of moles with molecular weight M i ; N i and M i being determined from the following equations Log M i = slope × t R + intercept with slope = -0.38 and intercept = 9.92 this gives Log M i = -0.38 × t R + 9.92
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