Agilent 7890a gc 5975c msd system

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 7890A GC/5975C MSD system is a gas chromatography-mass spectrometry (GC-MS) instrument designed for analytical applications. The system combines the Agilent 7890A gas chromatograph and the 5975C mass selective detector, providing high-performance separation and detection capabilities.

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Lab products found in correlation

Methoxyamine chloride in pyridine, by Merck Group (2 mentions) Rtx 5sil ms capillary column, by Restek (1 mentions) N methyl n trimethylsilyl trifluoroacetamide, by Merck Group (1 mentions) Db 5ms, by Agilent Technologies (1 mentions) Rtx 5sil ms column, by Restek (1 mentions) Hp 5ms fused silica capillary column, by Agilent Technologies (1 mentions) Hp 5ms capillary column, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Agilent 7890A GC/5975C MSD 7890A/5975C-GC/MSD system 7890A GC-5975C MSD Agilent 7890A GC/5975C Agilent 5975C GC MSD Agilent 7890A/5975C system Agilent 5975C MSD system Agilent 7890A GC system Agilent 7890A/5975C Agilent GC-MSD system

8 protocols using agilent 7890a gc 5975c msd system

Metabolite Derivatization and GC/MS Analysis

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Prior to GC/MS analysis, the samples were derivatized by methoxyamination and trimethylsilylation. For methoxyamination, 10 µL of 40 mg/mL methoxyamine chloride in pyridine (Sigma-Aldrich) was added to the samples and incubated for 90 min at 30 °C and 200 rpm. The samples were then trimethylsilylated by adding 45 µL of N-methyl-N-trimethylsilyltrifluoroacetamide (Sigma-Aldrich) with incubation for 30 min at 37 °C and 200 rpm.
For GC/MS, the derivatized metabolite samples were applied to an Agilent 7890A GC/5975C MSD system (Agilent Technologies) equipped with an RTX-5Sil MS capillary column (30 m × 0.25 mm, 0.25 µm film thickness; Restek, Bellefonte, PA) and an additional 10-m-long integrated guard column. One microliter of the derivatized sample was injected into the GC inlet in splitless mode. The oven temperature was initially set to 150 °C for 1 min, after which the temperature was increased to 330 °C at 20 °C/min, where it was held for 5 min. The mass spectra were recorded in a scan range 85–500 m/z at electron impact of 70 eV, and the temperatures of the ion source and transfer line were 230 and 280 °C, respectively.

Yun E.J., Oh E.J., Liu J.J., Yu S., Kim D.H., Kwak S., Kim K.H, & Jin Y.S. (2018). Promiscuous activities of heterologous enzymes lead to unintended metabolic rerouting in Saccharomyces cerevisiae engineered to assimilate various sugars from renewable biomass. Biotechnology for Biofuels, 11, 140.

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GC-MS Analysis of Organic Compounds

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Agilent 7890A GC/5975C MSD system (Agilent Technologies, Santa Clara, CA, USA); column: DB-5MS (30 × 0.25-mm i.d., film thickness 0.25 μm; Agilent Technologies); inlet temperature: 250 °C; oven temperature: 2 min at 60 °C, 10 °C/min to 300 °C, and 5 min at 300 °C; transfer line temperature: 280 °C; injection volume: 1 μL; injection mode: split (20:1) or splitless; carrier gas: He (1.2 mL/min); ionization conditions: electron ionization, 70 eV, 150 °C; mass range: m/z 40–500.

Sugie K.I., Kurakami D., Akutsu M, & Saito K. (2018). Rapid detection of tert-butoxycarbonyl-methamphetamine by direct analysis in real time time-of-flight mass spectrometry. Forensic Toxicology, 36(2), 261-269.

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GC-MS Analysis of Monosaccharide Derivatives

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Experiments were performed on an Agilent 7890A GC-5975C MSD system (Agilent Technologies, Santa Clara, CA) using a DB5-MS+10m Duraguard Capillary Column (30 m × 250 μm × 0.25 μm) as the stationary phase. The GC parameters used were as follows: split injection (1.0 μL sample at 100.0°C, 1.0 min—split ratio of 10:1); He carrier gas (40 cm s⁻¹ at constant velocity); 275.0°C transfer line temperature; oven temperature program: 1.0 min at 100°C, increased 20.0°C min⁻¹ to 200.0°C, then increased 3.0°C min⁻¹ to 325.0°C and held for 10.0 min, MS parameters: electron impact ionization at 70 eV, filament source temperature of 230.0°C, quadrupole temperature of 150.0°C, m/z scan range 50–600 at 2 spectra s⁻¹. Mass spectral signals were recorded after a 6.10 min solvent delay to avoid derivatization interferents, and turned off between 10.0 and 13.0 min to avoid saturation of the detector due to the high content of monosaccharides. A blank sample with the FAME standard mixture (FAME std) was also injected under the same GC conditions.

Carnevale Neto F., Pilon A.C., Selegato D.M., Freire R.T., Gu H., Raftery D., Lopes N.P, & Castro-Gamboa I. (2016). Dereplication of Natural Products Using GC-TOF Mass Spectrometry: Improved Metabolite Identification by Spectral Deconvolution Ratio Analysis. Frontiers in Molecular Biosciences, 3, 59.

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GC/MS Analysis of AR-Mediated Conversion

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To investigate the conversion of AHG and AB to AHGol and ABol, respectively, by in vitro AR enzyme reactions and by fermentation and the chemical conversion of AHGol to isosorbide, GC/MS analysis were performed. For the GC/MS analysis, 20 μL of enzymatic reaction products or fermentation cultures were dried using a centrifugal vacuum evaporator. Samples were derivatized by adding 10 μL of 40 mg/mL methoxyamine chloride in pyridine and incubating at 30 °C for 90 min. The samples were trimethylsilylated by adding 45 μL of N-methyl-N-(trimethylsilyl)-trifluoroacetamide and incubated at 37 °C for 30 min. The chemically derivatized samples were analyzed using an Agilent 7890A GC/5975C MSD system (Agilent Technologies, Wilmington, DE) equipped with a 30 m HP-5MS column with a 0.25 mm inner diameter, 0.25 μm film thickness, and a 10 m guard column. The 1 μL derivatized sample was injected into the GC column in splitless mode. The oven temperature of the GC was programmed to initially be at 80 °C for 1 min before ramping to 300 °C at 10 °C/min for 1 min. Electron ionization was performed at 70 eV. The temperatures of the ion source and transfer line were 250 and 280 °C, respectively. The mass spectra were recorded in a scan range of 20–700 m/z.

Kim D.H., Liu J.J., Lee J.W., Pelton J.G., Yun E.J., Yu S., Jin Y.S, & Kim K.H. (2020). Biological upgrading of 3,6-anhydro-L-galactose from agarose to a new platform chemical. Green chemistry : an international journal and green chemistry resource : GC, 22(5), 1776-1785.

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GC/MS Analysis of AB and Ethyl-AB

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To analyze AB and ethyl-AB using gas chromatography/mass spectrometry (GC/MS), 5 μL each of the samples containing 1 g/L of AB or ethyl-AB was dried using a centrifugal vacuum evaporator (Labconco, Kansas, MO, USA). The dried samples were derivatized by adding 10 μL of 40 mg/mL methoxyamine chloride in pyridine (Sigma-Aldrich, St. Louis, MO, USA) and then incubating at 30 °C for 90 min. The samples were then subjected to trimethylsilylation by adding 45 μL of N-methyl-N-(tri-methylsilyl)-trifluoroacetamide (MSTFA; Fluka, St. Louis, MO, USA) and then incubating at 37 °C for 30 min.
The derivatized samples were analyzed using an Agilent 7890A GC/5975C MSD system (Agilent Technologies) with a RTX-5Sil MS column (30 m length, 0.25 mm inner diameter, and 0.25 µm film thickness; Restek, Bellefonte, PA, USA) with an additional 10-m guard column. The GC/MS operating conditions were as follows. The derivatized sample of 1 μL was injected into the GC column in splitless mode. The GC oven temperature was initially programmed at 50 °C for 1 min, and then it rapidly increased by 20 °C/min to 280 °C, and then it was held constant for 5 min. Electron ionization was performed at 70 eV. The temperatures of the ion source and transfer line were 250 °C and 280 °C, respectively. Mass spectra were recorded in the scan range of 80–700 m/z.

Lee S.H., Yun E.J., Han N.R., Jung I., Pelton J.G., Lee J.E., Kang N.J., Jin Y.S, & Kim K.H. (2023). Production of Ethyl-agarobioside, a Novel Skin Moisturizer, by Mimicking the Alcoholysis from the Japanese Sake-Brewing Process. Marine Drugs, 21(6), 341.

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GC-MS Analysis of Brain Tissue

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GC-MS was carried out on an Agilent 7890A/5975C GC/MSD system (Agilent Technologies, Santa Clara, CA, USA) and an HP-5 MS fused silica capillary column (30 m × 0.25 mm × 0.25 μm; Agilent Technologies). Each 0.5-μL aliquot of the derivatized solution was injected into the system with an injector temperature of 280°C. The MS quadrupole temperature was set at 150°C, and the ion source temperature was set at 230°C. Helium carrier gas was used at a constant flow rate of 6.0 mL/min. The column temperature was initially kept at 70°C for 2 min, and then increased from 70 to 160°C at 6°C/min. The temperature was thereafter increased from 160 to 240°C at 10°C/min, and finally increased from 240 to 300°C at 20°C/min, and maintained for 6 min. Data acquisition was performed in the full scan mode from 50 to 600 m/z. Moreover, quality control samples were prepared by mixing equal amounts of brain tissue sample and were pretreated in the same manner as the real samples. One quality control sample was inserted after every 8–10 real sample runs.

Liu Y.Y., Zhou X.Y., Yang L.N., Wang H.Y., Zhang Y.Q., Pu J.C., Liu L.X., Gui S.W., Zeng L., Chen J.J., Zhou C.J, & Xie P. (2017). Social defeat stress causes depression-like behavior with metabolite changes in the prefrontal cortex of rats. PLoS ONE, 12(4), e0176725.

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Metabolic Profiling of Stressed Rat Hippocampus

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Hippocampus samples were obtained from stressed rats and corresponding controls after being anaesthetized by an intraperitoneal injection of 10% chloral hydrate (100 g/0.4 ml). For each depression model, an independent pool of case and control hippocampi was created. Metabolic profiling of the processed hippocampi was achieved using an Agilent 7890A/5975C GC/MSD System (Agilent Technologies Inc., USA). Details of the pretreatment of the hippocampus samples and GC-MS analysis were provided in our previous studies^{20 (link),21 (link)}.

Liu L., Zhou X., Zhang Y., Pu J., Yang L., Yuan S., Zhao L., Zhou C., Zhang H, & Xie P. (2018). Hippocampal metabolic differences implicate distinctions between physical and psychological stress in four rat models of depression. Translational Psychiatry, 8, 4.

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GC-MS Analysis of Organic Derivatives

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The procedures essentially referred to previous studies.26 (link),27 (link) Briefly, each 1 μl of derivative sample was injected into an Agilent 7890A/5975C GC/MSD system (Agilent Technologies Inc., USA) with an injector temperature of 280°C. A HP-5MS capillary column (30 m × 0.25 mm × 0.25 m; Agilent) was utilized to separate the derivatives. The flow rate of helium carrier gas was set to 6.0 mL/min. The column temperature was initially maintained at 60°C for 2 mins, and then increased from 60°C to 310°C through four heating gradients (60°C to 125°C at 8°C/min; 125°C to 190°C at 10°C/min; 190 to 210°C at 4°C/min; 210°C to 310°C at 20°C/min, respectively), and finally maintained for 8.5 mins. The MS quadrupole temperature and the ion source temperature were set to 150°C and 230°C, respectively. The collision energy was 70 eV. Mass data were acquired in the full scan model from 50 to 600 m/z. Moreover, quality control (QC) samples were prepared by mixing aliquots of the all samples to be a pooled sample, and then analyzed using the same method with the analytic samples. The QCs were injected at regular intervals (every 8 samples) throughout the analytical run to provide a set of data from which repeatability can be assessed. A random order of continuous sample analysis was used to avoid the error resulting from instrument signal fluctuations.

Xu S., Liu Y., Pu J., Gui S., Zhong X., Tian L., Song X., Qi X., Wang H, & Xie P. (2020). Chronic Stress in a Rat Model of Depression Disturbs the Glutamine–Glutamate–GABA Cycle in the Striatum, Hippocampus, and Cerebellum. Neuropsychiatric Disease and Treatment, 16, 557-570.

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