Formalin-fixed, paraffin-embedded labial and parotid salivary gland tissue samples were serially sectioned at 3 µm thickness and deparaffinized. Automatic staining was performed with H&E, and tissue samples were manually stained for CD3 (clone 2GV6, Roche), high-molecular-weight cytokeratin (hmwCK, clone 34βE12, Roche) and CD20 (clone L-26, Roche). Antigen retrieval was performed for 15 min in EDTA buffer (98°C, pH 8.0), and endogenous peroxidase was blocked. Pre-fixed dilutions of primary antibodies (1% BSA-PBS, Roche) were applied for 75 min. Primary antibodies were visualized by using 3,3′-diaminobenzidine (DAB) after incubation with a poly-horseradish peroxidase-labelled secondary antibody (Thermo Scientific). Tonsillar tissue was used as both a positive and negative control, as the tonsillar epithelium expresses hmwCK and does not express CD3 or CD20, and the tonsillar lymphoid tissue expresses CD3 and CD20 but does not express hmwCK.
Bsa pbs
Manufactured by Roche
BSA-PBS is a buffer solution used in various laboratory applications. It is composed of bovine serum albumin (BSA) and phosphate-buffered saline (PBS). BSA-PBS is commonly used for blocking, washing, and diluting samples in immunoassays and other protein-based experiments.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 clone 2gv6, by Roche (1 mentions)
Cd20 clone l26, by Roche (1 mentions)
Prolong diamond, by Thermo Fisher Scientific (1 mentions)
Alexa 568, by Thermo Fisher Scientific (1 mentions)
Nedd4l, by Cell Signaling Technology (1 mentions)
Snap surface alexa 488, by New England Biolabs (1 mentions)
Nedd4, by Santa Cruz Biotechnology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
2 protocols using bsa pbs
1
Comprehensive Immunohistochemical Characterization of Salivary Gland Tissues
2
SNAP-LGR5 and SNAP-FZD5 Colocalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HEK293T cells were grown on laminin‐coated glass coverslips. Cells were co‐transfected with 100 ng SNAP‐LGR5 or SNAP‐FZD5 and 150 ng myc‐NEDD4, HA‐NEDD4‐CS, myc‐NEDD4L, HA‐NEDD4L‐CA or pcDNA4 as a control with Fugene according to the manufacturer's instructions. After 24 h of transfection, cells were labelled with 1 μM SNAP‐Surface Alexa 488 (NEB) for 15 min at 4°C to block endocytosis. Cells were then immediately washed with fresh RPMI media and fixed in 4% paraformaldehyde. Cells were incubated with primary antibodies NEDD4 (Santa Cruz) or NEDD4L (Cell Signaling) for 1 h at RT followed by a secondary antibody conjugated to Alexa‐568 (Invitrogen) and DAPI (Sigma) in 2% BSA‐PBS (Roche). Cells were mounted in Prolong Diamond (Life technologies) and imaged using a DeltaVision Core system.
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