Serum cytokines from time-course experiments were measured by LEGENDPlex bead-based immunoassay using the Mouse Th Cytokine Panel (13-plex), per the manufacturer’s instructions (Biolegend). Serum IFN-γ levels on day 7 post-MCMV were determined via sandwich ELISA using the following antibodies: purified IFN-γ (clone R4–6A2, eBioscience), Biotin-IFN-γ (clone XMG1.2, eBioscience), and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Labs) (Wesley et al., 2005 (link)).
Mouse th cytokine panel 13 plex
Manufactured by BioLegend
The Mouse Th Cytokine Panel (13-plex) is a multiplex assay that measures 13 different cytokines related to T helper cell immune responses in mouse samples. The assay allows for the simultaneous quantification of multiple analytes in a single sample.
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2 protocols using mouse th cytokine panel 13 plex
1
Serum Cytokine Profiling in MCMV
2
Serum Cytokine Profiling in MCMV
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