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Goat antibodies

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Goat antibodies are a type of immunoglobulin produced by goats. They are a versatile tool used in various laboratory applications, such as immunoassays, affinity purification, and immunohistochemistry.

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2 protocols using goat antibodies

1

FGFR1 Signaling Antibody Characterization

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Goat antibodies against adenine nucleotide translocase (ANT, #sc-929) were from Santa Cruz Biotechnology, Dallas, TX, USA. Mouse monoclonal antibodies against cyclophilin (#MSA04) D and cytochrome c (#556433), were, respectively, from MitoSciences, Eugene, OR, USA, and BD Biosciences Pharmingen, Mississauga, ON, Canada. Rabbit anti-FGFR1 (#sc-121), anti-pY766 (#16309-R), anti-pY653/654 (#30262-R), all recognizing sites at the catalytic C-terminal domain, were from Santa Cruz Biotechnology. Neutralizing anti-FGFR1 antibody (#MAB125, ligand-binding domain) was from Millipore Sigma, Oakville, ON, Canada. Rabbit-affinity-purified anti-FGFR1 (#F5421) was from Sigma, and mouse monoclonal anti-FGFR1 (#30101; M19B2; QED A/B, ligand-binding domain) was from QED Bioscience Inc. (San Diego, CA, USA). Mouse anti-Shc (#610878) was from BD Transduction, while anti-pY239/240-Shc (sc-18074-R) from Santa Cruz Biotechnology. Donkey anti-rabbit horseradish peroxidase (HRP), #711-035-152, and anti-mouse HRP, #715-035-150, as well as anti-goat HRP, #705-035-147, were from Jackson Immuno Res. Lab. Secondary anti-rabbit antibodies used in immunoelectron microscopy were coupled to 10 nm gold particles (Sigma, Oakville, ON, Canada).
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2

Hypoxia-Induced Beclin1 Immunoprecipitation

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Cells were subjected to hypoxia for 6 hours, followed by isolation of cellular extracts as above. From each sample, containing 2 mg of total protein, Beclin1 was immunoprecipitated overnight with goat antibodies (Santa Cruz, 20 μl) using ExactoCruz system (Santa Cruz Biotechnology) according to manufacturer’s recommendations. Immunoprecipitates were lysed in 60 μl of 2x gel loading buffer, and subjected to electrophoresis (15 μl per lane) followed by Western blot analysis, using rabbit primary antibodies from Cell Signaling Technology.
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