Desethylatrazine (DEA), desisopropylatrazine, simazine, cyanazine, ATZ, propazine (PPZ), TER, MAA, ethylene glycol dimethacrylate (EGDMA), and 2,2′‐azobis‐2‐methylbutyronitrile (AIMN) were supplied by Sigma‐Aldrich (Madrid, Spain). HEMA was purchased from Merck (Darmstadt, Germany). ACN and methanol (MeOH) were from Scharlab (Barcelona, Spain). AIMN was recrystallized in MeOH and EGDMA and MAA were freed from stabilizers by distillation under reduced pressure prior to use. Purified water was obtained from a Milli‐Q purification system purchased from Millipore (Madrid, Spain). All other chemicals were of analytical reagent grade and used as received.
Methanol meoh
Manufactured by Scharlab
Sourced in Spain
Methanol (MeOH) is a clear, colorless, and volatile liquid chemical compound. It is the simplest alcohol, with the chemical formula CH3OH. Methanol serves as a solvent, fuel, and chemical intermediate in various industrial processes.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile acn, by Thermo Fisher Scientific (2 mentions)
Milli q purification system, by Merck Group (1 mentions)
Ethylene glycol dimethacrylate, by Merck Group (1 mentions)
2 2 azobis 2 methylbutyronitrile, by Merck Group (1 mentions)
Nylon syringe filters, by Scharlab (1 mentions)
α solanine, by Phytolab (1 mentions)
α chaconine, by Phytolab (1 mentions)
Dibutyltin dilaurate, by Merck Group (1 mentions)
Anhydrous magnesium sulfate mgso4, by Thermo Fisher Scientific (1 mentions)
Sodium tetraphenylborate nabph4, by Merck Group (1 mentions)
1 8 diazabicyclo 5.4.0 undec 7 ene dbu, by Thermo Fisher Scientific (1 mentions)
2 2 dimethoxy 2 phenylacetophenone dmpa, by Thermo Fisher Scientific (1 mentions)
Deuterated chloroform cdcl3, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Lc ms grade formic acid, by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
Simvastatin, by Merck Group (1 mentions)
Salicylic acid, by Thermo Fisher Scientific (1 mentions)
Cholic acid, by Merck Group (1 mentions)
Paracetamol, by Merck Group (1 mentions)
7 protocols using methanol meoh
1
Analytical Reagent Purification Protocol
2
HPLC Analysis of Potato Glycoalkaloids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HPLC-grade solvents such as methanol (MeOH) and ethanol (EtOH) were obtained from Scharlab (Barcelona, Spain), and acetonitrile (ACN) was obtained from Fisher Chemical (Madrid, Spain). Monosodium phosphate (NaH2PO4) and disodium phosphate (Na2HPO4) were purchased from Panreac (Barcelona, Spain). Ultrapure deionized water with a resistance of 18.2 MΩ cm was obtained by a Milli-Q system (Billerca, MA, USA). Nylon syringe filters (0.45 μm, 13 mm) were purchased from Scharlab (Barcelona, Spain). For standard solutions preparation, α-solanine (>99%) and α-chaconine (>99%), purchased from Phytolab (Madrid, Spain), were used. Individual stock standard solutions of α-solanine and α-chaconine were prepared at a concentration of 200 μg/mL in MeOH and stored at −20 °C. The calibration standards were prepared at the appropriate concentrations (1, 2.5, 5, 10, 25, 50, and 100 mg/L for both analytes) by serial dilution of the stock solutions in MeOH. The prepared solutions were stored in the dark at a temperature of −20 °C.
3
Synthesis and Characterization of Novel Polyurethanes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
(R)-(+)-limonene (LM), hexamethylene diisocyanate (HDI), dibutyltin dilaurate (DBTDL), and sodium tetraphenylborate (NaBPh4) were obtained from Sigma Aldrich (Saint Louis, MO, USA). Squalene (SQ) and 1,8-diazabicyclo(5.4.0)undec-7-ene (DBU) were obtained from Alfa Aesar (Thermo Fisher, Kandel, Germany). Thioacetic acid (TAA) and 2,2-dimethoxy-2-phenylacetophenone (DMPA) were obtained from Acros Organics (Thermo Fisher Scientific, Geel, Belgium). Anhydrous magnesium sulfate (MgSO4) was obtained from (Thermo Fisher Scientific, Geel, Belgium). Sodium hydroxide (NaOH), hydrochloric acid (HCl), chloroform (CHCl3), and methanol (MeOH) were obtained from Scharlab (Barcelona, Spain). All products were used as received. BGDBU, which is a base generator, was synthesized from DBU and sodium tetraphenyl borate according to a reported methodology [24 (link)].
4
Synthesis of Photosensitive Polymers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methyl methacrylate (MMA ) (99%),
(2-acetoacetoxy)ethyl methacrylate (AEMA ) (95%), 2.2′-azo(2-methylpropionitrile)
(AIBN ) (≥98%), p-carboxybenzenesulfon-azide
(p-CBSA ) (97%), triethylamine (Et 3 N ) (>99%), HPS (98%), 1,1-dimethyl-2,5-bis(4-(azidomethyl)phenyl)-3,4-diphenylsilole
(Silole-N 3 ) (96%), ethyl acetate
(EtOAc ) (anhydrous, 99.8%), diethyl ether (Et 2 O ) (ACS reagent, anhydrous,
>99.0%), dichloromethane (CH 2 Cl 2 ) (anhydrous, ≥99.8%),
and deuterated chloroform (CDCl 3 ) (99.96 atom % D, containing 0.03% (v/v) tetramethylsilane) were
purchased from Sigma-Aldrich and used, unless specified, as received.
2-Cyanoprop-2-yl-dithiobenzoate (≥97%) was purchased from Strem
Chemicals. Methanol (MeOH ) (synthesis grade) and THF (HPLC grade) were purchased from Scharlab. AIBN was recrystallized from methanol. MMA was purified
by distillation before use.AEMA was purified by passing
through alumina.
(2-acetoacetoxy)ethyl methacrylate (
(
(
(
(
>99.0%), dichloromethane (
and deuterated chloroform (
purchased from Sigma-Aldrich and used, unless specified, as received.
2-Cyanoprop-2-yl-dithiobenzoate (≥97%) was purchased from Strem
Chemicals. Methanol (
by distillation before use.
through alumina.
5
LC-MS System Validation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The LC–MS grade formic acid and acetonitrile (ACN) used for the mobile phases’ preparation were purchased from Fisher Scientific (Pittsburgh, PA, USA) and Scharlab, S.L. (Barcelona, Spain), respectively. The methanol (MeOH) used in the sample and reagent solution preparation was obtained from Scharlab, S.L. (Barcelona, Spain). In addition, ultra-high-purity water, obtained from tap water pre-treated by Elix reverse osmosis and a Milli-Q system from Millipore (Bedford, MA, USA), was used for mobile phase, reagent solutions, and sample preparation.
Standard reagents used to assess the proper LC–MS system operation were from different manufacturers: paracetamol, cholic acid, (±) verapamil hydrochloride, simvastatin, reserpine, and leucine enkephalin acetate salt hydrate were provided by Sigma-Aldrich (Steinheim, Germany); caffeine and salicylic acid were supplied by Alfa Aesar (Karlsruhe, Germany) and Fluka Analytical (Bucharest, Romania), respectively. Finally, sodium fluvastatin was kindly supplied by Novartis (Basel, Switzerland). A system suitability test solution (SST) was prepared with the nine compounds at a final concentration of 100 ng/mL in MeOH:H2O 2:1 (v/v); this solvent composition was chosen as this is similar to the final solution (supernatant) of plasma samples.
Standard reagents used to assess the proper LC–MS system operation were from different manufacturers: paracetamol, cholic acid, (±) verapamil hydrochloride, simvastatin, reserpine, and leucine enkephalin acetate salt hydrate were provided by Sigma-Aldrich (Steinheim, Germany); caffeine and salicylic acid were supplied by Alfa Aesar (Karlsruhe, Germany) and Fluka Analytical (Bucharest, Romania), respectively. Finally, sodium fluvastatin was kindly supplied by Novartis (Basel, Switzerland). A system suitability test solution (SST) was prepared with the nine compounds at a final concentration of 100 ng/mL in MeOH:H2O 2:1 (v/v); this solvent composition was chosen as this is similar to the final solution (supernatant) of plasma samples.
6
Targeted LC-MS/MS Quantification of Olive Phenolics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LC grade reagents and solvents were used in this research. Ammonium fluoride from Fisher Scientific (Hampton, NH, USA) was used as the ionizing agent. Methanol (MeOH) from Scharlab (Barcelona, Spain) and deionized water (18 mΩ • cm) from a Millipore Milli-Q water purification system (Merck Millipore, Bedford, MA, USA) were used for the preparation of chromatographic mobile phases.
Hydroxytyrosol and tyrosol were purchased from Extrasynthese (Genay, France), whereas secoiridoid derivatives oleacein and oleocanthal were acquired from Phytolab (Vestenbergsgreuth, Germany). On the other hand, oleuropein aglycone and ligstroside aglycone (both as monoaldehyde closed isomers) were purchased from TRC (Ontario, Canada). Syringaldehyde from Sigma-Aldrich (St. Louis, MO, USA) was used as an internal standard (IS) to control the LC-MS/MS performance.
Hydroxytyrosol and tyrosol were purchased from Extrasynthese (Genay, France), whereas secoiridoid derivatives oleacein and oleocanthal were acquired from Phytolab (Vestenbergsgreuth, Germany). On the other hand, oleuropein aglycone and ligstroside aglycone (both as monoaldehyde closed isomers) were purchased from TRC (Ontario, Canada). Syringaldehyde from Sigma-Aldrich (St. Louis, MO, USA) was used as an internal standard (IS) to control the LC-MS/MS performance.
7
Targeted Analysis of Perfluorinated Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The target analytes included two perfluorosulfonates which were PFHxS and PFOS and five perfluorocarboxylates which included PFHxA, PFHpA, PFOA, PFNA and PFDA. Internal Standards (I.S.) in methanol included 13 C 2 -PFHxA, 13 C 4 -PFHpA, 13 C 8 -PFOA, 13 C 5 -PFNA, 13 C 6 -PFDA, 18 O 2 -PFHxS and 13 C 8 -PFOS. A list with abbreviations of the nonlabelled and labelled standards is shown in Table S1 of the Supporting information (SI).
The analytical standards and labelled standards were provided as a mixture in methanol by Prof. De Voogt and his team and had originally been procured by Campro Scientific, Veenendaal, The Netherlands. Methanol (MeOH) of LC-MS grade was obtained from Scharlab, while ammonium acetate (AcNH 4 : 77.08 g/mol; 98%) and sodium thiosulfate pentahydrate (Na 2 S 2 O 3 •5H 2 O: 248.18 g/mol; N 99.5%) were obtained from Sigma-Aldrich (Steinheim, Germany). Ultra-pure water having a conductivity of 18 MΩ/cm was prepared using the Elga Purelab Classic purification system.
The analytical standards and labelled standards were provided as a mixture in methanol by Prof. De Voogt and his team and had originally been procured by Campro Scientific, Veenendaal, The Netherlands. Methanol (MeOH) of LC-MS grade was obtained from Scharlab, while ammonium acetate (AcNH 4 : 77.08 g/mol; 98%) and sodium thiosulfate pentahydrate (Na 2 S 2 O 3 •5H 2 O: 248.18 g/mol; N 99.5%) were obtained from Sigma-Aldrich (Steinheim, Germany). Ultra-pure water having a conductivity of 18 MΩ/cm was prepared using the Elga Purelab Classic purification system.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!