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Micropure uv

Manufactured by Thermo Fisher Scientific
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The Micropure UV is a laboratory equipment designed for the purification of DNA and RNA samples. It utilizes ultraviolet (UV) light to effectively remove contaminants and inhibitors from nucleic acid preparations, ensuring the purity and quality of the samples for downstream applications.

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Lab products found in correlation

1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Formic acid, by Merck Group (1 mentions) Ammonium formate, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Glacial acetic acid, by Mallinckrodt (1 mentions) Ethyl acetate, by Merck Group (1 mentions) Dimethylformamide dmf, by Merck Group (1 mentions) Ultrapure hno3, by Avantor (1 mentions) 15 ml polystyrene tube, by Corning (1 mentions) Ethyl acetate, by Thermo Fisher Scientific (1 mentions) Acetone, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Ammonium acetate, by Thermo Fisher Scientific (1 mentions) 2 propanol, by Thermo Fisher Scientific (1 mentions) 1 palmitoyl 2 oleoyl 3 linoleoyl rac glycerol, by Merck Group (1 mentions) Li heparin, by Innovative Research (1 mentions) Triolein, by Merck Group (1 mentions) Splash lipidomix mass spectrometry standard, by Avanti Polar Lipids (1 mentions) Hplc grade methanol, by Avantor (1 mentions)

6 protocols using micropure uv

1

Phospholipid and Fatty Acid Standards Preparation

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1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (PC 16:0/18:1(9Z)) and 1-stearoyl-2-oleoyl-sn-glycero-3-phosphate (PA 18:0/18:1(9Z)) dissolved in 10 mg/mL chloroform were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Stock solutions in chloroform were diluted with isopropanol (LC grade; Macron Fine Chemicals; Center Valley, PA, USA) before diluting to the final working solution. Primary organic solvents used in ESI working solutions were all LC grade and consisted of methanol (Macron Fine Chemicals), acetonitrile (Sigma Aldrich; St. Louis, MO, USA), and isopropanol. Ultrapure H2O was obtained from a purification system at 0.03 μS·cm (model: Micropure UV; Thermo Scientific; San Jose, CA, USA). Ammonium hydroxide (28–30% as NH3; Macron Fine Chemicals), glacial acetic acid (Mallinckrodt Chemicals; Hazelwood, MO, USA), formic acid and ammonium formate (Sigma Aldrich, St. Louis, MO, USA) were used as solution modifiers to enhance lipid ionization via ESI. 1-Stearoyl-2-arachidonoyl (PC 17:0/20:4), cis-vaccenic acid (FA 18:1 (11Z)), and oleic acid (FA 18:1 (9Z)) were purchased as purified oils and dissolved in chloroform for final concentrations of 1 mg/mL and subsequently diluted into working solutions.
Stinson C.A., Zhang W, & Xia Y. (2017). UV Lamp as a Facile Ozone Source for Structural Analysis of Unsaturated Lipids via Electrospray Ionization-Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 29(3), 481-489.
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2

Algae Culture and Chlorine Pretreatment

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TCP (98%) was purchased from Aladdin (Shanghai, China), and its stock solution of 12 mM was prepared by pure water (Micropure UV, Thermo Fisher Scientific, USA) containing 0.01 mM NaOH. M. aeruginosa (No. FACHB-909) was obtained from the Institute of Hydrobiology, Chinese Academy of Sciences and cultured in BG-11 media according to our previous research (Wang et al., 2016 (link)). Cells were harvested by centrifugation at 4,500 rpm for 10 min and re-suspended with 15 mM NaClO4 solution twice to remove residual BG-11 media. Then the algae solution was diluted to 1 × 106 cells/mL by adding 15 mM NaClO4 solution (Liu et al., 2018 (link)). All the other chemical reagents of analytical grade at least were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
Wang J., Wan Y., Yue S., Ding J., Xie P, & Wang Z. (2020). Simultaneous Removal of Microcystis aeruginosa and 2,4,6-Trichlorophenol by UV/Persulfate Process. Frontiers in Chemistry, 8, 591641.
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3

Synthesis and Characterization of Diverse Diacylglycerols

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All the chemical reagents and solvents were purchased from commercial sources and were used without further purification. DAGs 16:0/18:1(9Z)/0:0, 15:0/18:1-d7(9Z)/0:0, 18:0/20:4(5Z,8Z,11Z,14Z)/0:0, 18:1(9Z)/18:1(9Z)/0:0, and 18:1(9Z)/0:0/18:1(9Z) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). DAGs 14:1(9Z)/14:1(9Z)//0:0, 16:1(9Z)//16:1(9Z)//0:0, 17:1(9Z)/17:1(9Z)/0:0, 18:1(6Z)/18:1(6Z)/0:0, 18:2-(9Z,12Z)/18:2(9Z,12Z)/0:0, 18:3(9Z,12Z,15Z)/18:3-(9Z,12Z,15Z)/0:0, 20:1(9Z)/20:1(9Z)/0:0, 22:1(9Z)/22:1(9Z)/0:0, and 24:1(9Z)/24:1(9Z)/0:0 were purchased from Nu-Check Prep, Inc. (Elysian, MN, USA). Thioglycolic acid (TGA), sodium-2-mercaptoethanesulfonate (MESNA), cysteamine (CA) hydrochloride, dimethylformamide (DMF), 2,2-dimethoxy-2-phenylacetophenone (DMPA), hydrochloric acid, and ethyl acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Deionized water was obtained from a purification system at 0.03 μS·cm (model: Micropure UV; Thermo Scientific; San Jose, CA, USA). Pooled human plasma (Li Heparin used as anticoagulant) was purchased from Innovative Research (Novi, MI, USA).
Adhikari S., Zhang W., Xie X., Chen Q, & Xia Y. (2018). Shotgun Analysis of Diacylglycerols Enabled by Thiol–ene Click Chemistry. Analytical chemistry, 90(8), 5239-5246.
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4

Trace Element Analysis in Tissues

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One mL of whole blood from each subject was collected into a 15 mL polystyrene tube (Corning, Glendale, AZ, USA), and was added to 2 mL of ultrapure HNO3 (VWR, Leuven, Belgium) and digested on a heat block (ModBlock, CPI International, Santa Rosa, CA, USA) at 80 °C until complete dissolution. The digests were further diluted with ultrapure deionized water (Micro Pure UV, Thermo Scientific Barnstead, Langenselbold, Germany).
Approximately 0.150 g of cancer and non-cancer tissues were dried at 105 °C overnight (the water content was ca. 50%). Further, samples were weighed in 15 mL polystyrene tubes, added with 2 mL of ultrapure HNO3 (VWR), digested on a heat block at 80 °C until complete dissolution (ModBlock), and then diluted with ultrapure, deionized water for analysis.
Forte G., Pisano A., Bocca B., Fenu G., Farace C., Etzi F., Perra T., Sabalic A., Porcu A, & Madeddu R. (2024). Toxic Metal and Essential Element Concentrations in the Blood and Tissues of Pancreatic Ductal Adenocarcinoma Patients. Toxics, 12(1), 32.
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5

Lipidomics Standards for Plasma Analysis

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Triolein (TG (18:1(9Z)/18:1(9Z)/18:1(9Z)), triα-linolenic acid (TG (18:3(9Z, 12Z, 15Z)/18:3(9Z, 12Z, 15Z)/ 18:3(9Z, 12Z, 15Z))), and 1-Palmitoyl-2-oleoyl-3-linoleoyl-rac-glycerol (TG (16:0/18:1(9Z)/18:2(9Z, 12Z))) standards species were purchased from Sigma Aldrich. SPLASH Lipidomix Mass Spectrometry Standard was purchased from Avanti Polar Lipids (Alabaster, AL, US). Pooled human plasma (anticoagulant: Li Heparin) was purchased from Innovative Research (Novi, MI, US). Acetone, methanol, ethyl acetate, acetonitrile, 2-propanol, and ammonium acetate were purchased from Fisher Chemical (Pittsburgh, PA, US). Isooctane and chloroform were purchased from Beijing Tong Guang Fine Chemicals Company (Chaoyang Dist., Beijing, China). A purification system at 0.03 μS cm (model: Micropure UV; Thermo Scientific; San Jose, CA, USA) was used to obtain the deionized water.
Franklin E.T, & Xia Y. (2020). Structural elucidation of triacylglycerol using online acetone Paternò–Büchi reaction coupled with reversed-phase liquid chromatography mass spectrometry. The Analyst, 145(20), 6532-6540.
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6

HPLC-based Extraction and Purification of MCD and MCC

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HPLC-grade methanol (Honeywell Burdick & Jackson, Seoul, Korea) was purchased and deionized water was prepared with a water purification system (MicroPure UV Thermo Scientific, Budapest, Hungary). MCC and MCD were extracted as described by Srisook et al. (2017) . The purity of MCD (Supplementary Figure S1) and MCC (Mankhong et al., 2019) determined by HPLC was more than 97%.
Srisook K., Malapong C., Sawai P., & Srisook E. (2021). Validation of quantitative RP-HPLC-DAD method and extraction optimization of 4-methoxycinnamyl p-coumarate and trans-4- methoxycinnamaldehyde in Etlingera pavieana rhizomes. Journal of Applied Pharmaceutical Science.
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