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Cat assay kit

Manufactured by Solarbio
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Sourced in China

The CAT assay kit is a laboratory instrument used to measure the activity of the enzyme catalase (CAT) in biological samples. Catalase is an important antioxidant enzyme that helps protect cells from oxidative stress. The CAT assay kit provides a quantitative method for determining the catalase activity in various sample types, such as cell lysates, tissue homogenates, or purified enzyme preparations.

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Lab products found in correlation

Sod assay kit, by Solarbio (3 mentions) Reduced gsh assay kit, by Solarbio (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Power sybr green master mix, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit with gdna eraser, by Takara Bio (1 mentions) Propidium iodide, by Merck Group (1 mentions) 2 7 dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Radio immunoprecipitation assay ripa buffer, by Beyotime (1 mentions) Tg content detection kit, by Solarbio (1 mentions) T aoc assay kit, by Solarbio (1 mentions) Microplate reader, by Tecan (1 mentions) Gsh assay kit, by Solarbio (1 mentions) Tc assay kit, by Solarbio (1 mentions) Mda assay kit, by Solarbio (1 mentions)

7 protocols using cat assay kit

1

Antioxidant Assays and Cell Viability

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DPPH was purchased from Shanghai Chemical Reagent Co. (Shanghai, China); ABTS was purchased from Hefei Bomei Biotechnology Co., Ltd. (Hefei, China); Dulbecco’s Modified Eagle’s Medium (DMEM), trypsin-EDTA (0.25%), fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and penicillin–streptomycin were bought from Gibco, Life Technologies (Grand Island, NY, USA). Radio immunoprecipitation assay (RIPA) buffer, BCA protein assay kit, SOD, MDA, GPx, oxidized glutathione (GSSG) assay kits, and total antioxidant capacity assay kit with ferric-reducing ability of plasma (FRAP) method were all obtained from Beyotime Biotechnology (Shanghai, China). CAT assay kit and GR assay kit were bought from Solarbio Science & Technology (Beijing, China). 2′,7′-Dichlorofluorescein diacetate (DCFH-DA) and propidium iodide (PI) were purchased from Sigma Chemical Company (St. Louis, MO, USA). TRIGene regent was bought from GenStar (Beijing, China). PrimeScript™ RT reagent kit with gDNA eraser was bought from Takara (Dalian, China). Power SYBR green master mix was bought from Thermo Fisher Scientific (Wilmington, DE, USA).
Jiang J., Zhu Y., Wang M, & Chen J. (2023). Protective Effect of Chinese Bayberry (Myrica rubra Sieb. et Zucc.) Pomace Wine on Oxidative Stress of Hydrogen Peroxide by Regulating Keap1/Nrf2 Pathway in HepG2 Cells. Foods, 12(9), 1863.
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2

Liver Metabolic Profile Analysis

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Approximately 30 mg liver tissue was ground into homogenate to detect triglyceride (TG), free fatty acid (FFA), glutathione (GSH), SOD, and catalase (CAT) levels by TG content detection kit (Cat# BC0625), FFA content detection kit (Cat# BC0595), reduced GSH assay kit (Cat# A006-2-1), SOD assay kit (Cat# A001-3-2), and CAT assay kit (Cat# A007-1-1), which were from Solarbio (Beijing, China) and Nanjing Jiancheng Bioengineering Insititute (Nanjing, China).
Wang M., Zhang F., Zhou J., Gong K., Chen S., Zhu X., Zhang M., Duan Y., Liao C., Han J, & Yin Z. (2023). Glabridin Ameliorates Alcohol-Caused Liver Damage by Reducing Oxidative Stress and Inflammation via p38 MAPK/Nrf2/NF-κB Pathway. Nutrients, 15(9), 2157.
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3

Antioxidant Activity Quantification

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According to the manufacturer’s requirements, 24 h after cell transfection, a T-AOC assay kit (Solarbio), CAT assay kit (Solarbio), and reduced GSH assay kit (Solarbio) were used to detect the levels of T-AOC, CAT, and GSH, respectively. The absorption value was measured with a microplate reader (Tecan, Mannedorf, Switzerland). The T-AOC and activity of GSH and CAT were calculated based on the absorption value and standard curve.
Yu W., Jiang H., Liu F., Li Z., Xu L., Liu C., Lv W., Wang J., Gao Y., Liang S., Kim N.H., Zhang J., Chen C, & Yuan B. (2022). KRAS Affects the Lipid Composition by Regulating Mitochondrial Functions and MAPK Activation in Bovine Mammary Epithelial Cells. Animals : an Open Access Journal from MDPI, 12(22), 3070.
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4

Catalase and GSH Assay Protocol

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Catalase was measured by using the CAT Assay Kit (Solarbio, China), and GSH was measured by using the GSH Assay Kit (Solarbio, China) according to the manufacturer's instructions.
Lou Z.L., Zhang C.X., Li J.F., Chen R.H., Wu W.J., Hu X.F., Shi H.C., Gao W.Y, & Zhao Q.F. (2021). Apelin/APJ-Manipulated CaMKK/AMPK/GSK3β Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps. Oxidative Medicine and Cellular Longevity, 2021, 8836058.
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5

Quantifying Antioxidant Enzyme Activity

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After the freezing process, fresh leaves were immediately sampled to detect the antioxidant enzyme activity, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). The measurements were conducted using the SOD assay kit (Solarbio, China; Cat#BC0170), POD assay kit (Solarbio, China; Cat#BC0090), and CAT assay kit (Solarbio, China; Cat#BC0200) following the manufacturer’s protocols. A UV-6000PC UV-Visible Spectrophotometer (Shanghai, China) was used to measure the absorbance values. Six biological replicates for each sample were carried out through the whole process.
Peng Z., Wang Y., Zuo W.T., Gao Y.R., Li R.Z., Yu C.X., Liu Z.Y., Zheng Y., Shen Y.Y, & Duan L.S. (2022). Integration of Metabolome and Transcriptome Studies Reveals Flavonoids, Abscisic Acid, and Nitric Oxide Comodulating the Freezing Tolerance in Liriope spicata. Frontiers in Plant Science, 12, 764625.
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6

Evaluation of Semen Antioxidant and Calcium Levels

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The CAT assay kit (BC 0205), SOD assay kit (BC 0175), TC assay kit (BC 1980), and MDA assay kit (BC0025) were purchased from Solarbio Science&Technology Co., Ltd. (Beijing, China), T-AOC assay kit (S0121) was purchased from Beyond Biotechnology Co., Ltd. (Shanghai, China), and the Ca2+ assay kit (R22060) was purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). A 20 µL semen sample was mixed with 1 mL of the extract, and the semen sample was ground with a homogenizer for 5 min. The supernatant was centrifuged, and the levels of CAT, SOD, TC, MDA, T-AOC, and Ca2+ were measured using a spectrophotometer or enzyme-linked immunosorbent assay according to the manufacturer’s instructions.
Bai H., Zhang Z., Shen W., Fu Y., Cao Z., Liu Z., Yang C., Sun S., Wang L., Ling Y., Zhang Z, & Cao H. (2023). Metabolomics Analysis of Sodium Salicylate Improving the Preservation Quality of Ram Sperm. Molecules, 29(1), 188.
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7

Superoxide Anion Scavenging Assay

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The superoxide anion scavenging activity was assessed with a CAT assay kit (Solarbio, China). The PSCZ, PSZ, PCZ concentrations (100 μg/ml) test was performed according to the instructions provided with the kit. The absorbance at 405 nm was measured using a quartz colorimetric dish reader after incubating at room temperature for 10 min.
Hou X., Shi J., Zhang J., Wang Z., Zhang S., Li R., Jiang W., Huang T., Guo J, & Shang W. (2022). Treatment of Acute Kidney Injury Using a Dual Enzyme Embedded Zeolitic Imidazolate Frameworks Cascade That Catalyzes In Vivo Reactive Oxygen Species Scavenging. Frontiers in Bioengineering and Biotechnology, 9, 800428.
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