Standard western blotting protocol was used. Gastroctrocnemius muscle was lysed with ice-cold RIPA buffer in the presence of 1X protease inhibitor cocktail (Sigma). For each sample, 10 µg to 100 µg of total protein was loaded onto 4–12% SDS-polyacrylamide gradient gels (Invitrogen), and transferred to PVDF membranes. The membranes were blocked for 1 h with 1% BSA in PBST and incubated with various antibodies against dystrophin (Abcam), β-dystroglycan (β-DG) (Santa Cruz), caveolin-3 (BD Biosciences), dysferlin (Abcam), myosin (Iowa Hybridoma Bank), MG53 (Novus Biologicals), and Lamp1 (ID4B; Iowa Hybridoma Bank) in PBST. The blots were detected by using Peroxidase-conjugated anti-rabbit/mouse/rat secondary antibody with an enhanced chemiluminescence reagent (Amersham Pharmacia Biotech).
Peroxidase conjugated anti
Manufactured by Cytiva
Peroxidase-conjugated anti is a laboratory reagent used in various immunoassay techniques. It consists of an antibody molecule conjugated to the enzyme peroxidase. The primary function of this product is to serve as a detection tool, enabling the visualization and quantification of target analytes in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Dystrophin, by Abcam (2 mentions)
Dysferlin, by Abcam (2 mentions)
Gradient sds polyacrylamide gel, by Thermo Fisher Scientific (2 mentions)
β dystroglycan β dg, by Santa Cruz Biotechnology (2 mentions)
Caveolin 3, by BD (2 mentions)
Enhanced chemiluminescence reagent, by Cytiva (2 mentions)
Protease inhibitor cocktail, by Merck Group (2 mentions)
2 protocols using peroxidase conjugated anti
1
Muscle Protein Expression Analysis
2
Muscle Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Standard western blotting protocol was used. Gastroctrocnemius muscle was lysed with ice-cold RIPA buffer in the presence of 1X protease inhibitor cocktail (Sigma). For each sample, 10 µg to 100 µg of total protein was loaded onto 4–12% SDS-polyacrylamide gradient gels (Invitrogen), and transferred to PVDF membranes. The membranes were blocked for 1 h with 1% BSA in PBST and incubated with various antibodies against dystrophin (Abcam), β-dystroglycan (β-DG) (Santa Cruz), caveolin-3 (BD Biosciences), dysferlin (Abcam), myosin (Iowa Hybridoma Bank), MG53 (Novus Biologicals), and Lamp1 (ID4B; Iowa Hybridoma Bank) in PBST. The blots were detected by using Peroxidase-conjugated anti-rabbit/mouse/rat secondary antibody with an enhanced chemiluminescence reagent (Amersham Pharmacia Biotech).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!