Calcium carbonate

Manufactured by Fujifilm

Sourced in Japan, United States

Calcium carbonate is a mineral compound that is commonly used in laboratory equipment. It is a white, crystalline solid that is insoluble in water. Calcium carbonate serves as a source of calcium and is often used in various laboratory applications, including as a pH buffer, precipitating agent, and filler material.

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5 protocols using calcium carbonate

Calcium Carbonate Synthesis in Methanol

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A weight of 50 mg of calcium carbonate
(Wako Pure Chemical Industries,
Ltd.) and 50 mL of methanol (Wako Pure Chemical Industries, Ltd.)
was added to a 130 mL Pyrex glass container, which was subsequently
sealed and purged with nitrogen to remove any traces of reactive gases.
After that, 500 μL of 1 M sulfuric acid was added to the container
using a micropipette, and the resulting mixture was stirred using
a magnetic stirring bar on a stir plate. The reaction was allowed
to proceed for 425 min at a temperature of 20 °C. For comparison,
the same experiment was conducted using distilled water as the solvent.
The samples synthesized in methanol were filtered and washed with
methanol, and the samples synthesized in distilled water were filtered
and washed with distilled water.

Fukugaichi S, & Matsue N. (2018). One-Step Synthesis of Calcium Sulfate Hemihydrate Nanofibers from Calcite at Room Temperature. ACS Omega, 3(3), 2820-2824.

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Characterization of Chemical Compounds

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White Coat was purchased from Shiraishi Calcium. Calcium carbonate, dodecane, pentadecane, hexadecane, heptadecane, nonyl acetate, dichloromethane, tetramethylsilane, and deuterated chloroform were obtained from Wako Pure Chemicals (Osaka, Japan). Other standards were obtained as follows: tridecane and tetradecane (Tokyo Chemical Industry, Tokyo, Japan), farnesene (mixture of isomers) and hexyl 2-methylbutanoate (Sigma-Aldrich, St. Louis, MO, USA), TXIB (Matrix Scientific, Columbia, SC, USA), texanol (Santa Cruz Biotechnology, Dallas, TX, USA).

Kazama H., Oohata Y., Takanashi T., Tokoro M., Ishiguri Y., Mori N, & Yoshinaga N. (2020). Inhibitory substances contained in calcium carbonate wettable powder on the oviposition of the peach fruit moth, Carposina sasakii. Journal of Pesticide Science, 45(1), 16-23.

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In Vitro Phosphate Solubility Evaluation

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PA21 (Vifor Pharma, Glattbrugg, Switzerland), lanthanum carbonate hydrate (Alfa Aesar, Lancashire, UK), sevelamer hydrochloride (AK Scientific, Inc., Union City, CA, USA), calcium carbonate (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), and ferric citrate hydrate (Nacalai Tesque, Inc., Kyoto, Japan) were used for the experiments. For the in vivo studies, each investigational drug was dissolved or suspended in 0.5% (w/v) methylcellulose (MC) solution (FUJIFILM Wako Pure Chemical Corporation). After phosphate (FUJIFILM Wako Pure Chemical Corporation), disodium hydrogen phosphate dodecahydrate (FUJIFILM Wako Pure Chemical Corporation), and sodium dihydrogen phosphate dihydrate (FUJIFILM Wako Pure Chemical Corporation) were diluted or dissolved in distilled water, the resulting phosphate solution was mixed and adjusted to pH 2, 3, 4, 5, 6, 7, or 8 [16 (link)], simulating the conditions in the digestive tract.

Yaguchi A., Akahane K., Tsuchioka K., Yonekubo S., Yamamoto S., Tamai Y., Tatemichi S, & Takeda H. (2019). A comparison between the combined effect of calcium carbonate with sucroferric oxyhydroxide and other phosphate binders: an in vitro and in vivo experimental study. BMC Nephrology, 20, 465.

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Calcium Carbonate and Maltose Monohydrate Protocol

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Calcium carbonate and maltose monohydrate were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Catalase and glucose oxidase were purchased from Amano Enzyme Inc. (Aichi, Japan). CaMb powder was prepared according to our previous studies.⁴⁾ ⁵⁾ The purity of CaMb was confirmed to be > 99 % by high-performance liquid chromatography (HPLC).

Anantawittayanon S., Mochizuki T., Harada Y., Fukami K, & Kawai K. (2023). Browning Behavior of Calcium Maltobionate-water System. Journal of Applied Glycoscience, 70(3), 75-80.

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Isolation and Identification of Lactic Acid Bacteria

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Samples obtained from kimchi and rice bran were streaked on deMan, Rogosa and Sharpe (MRS) agar (Becton, Dickinson and Company, MD, USA) plates containing 0.5% calcium carbonate (Wako Pure Chemical Industries, Osaka, Japan). After incubation at 30°C under anaerobic conditions, bacterial colonies with transparent halos were isolated and further characterized. The bacterial colonies were cultured in 15 mL of MRS broth (Becton, Dickinson and Company) under anaerobic conditions at 30°C. Bacterial pellets were collected by centrifugation, suspended into 0.9% NaCl (Wako Pure Chemical Industries), mixed well with the same volume of 80% glycerol (Wako Pure Chemical Industries), and stored at -80°C. The bacterial 16S rDNA was amplified by colony polymerase chain reaction. A homology search was carried out on a database using BLAST. Bacterial species exhibiting the highest sequence homology were identified.

Ishii M., Nishida S., Kataoka K., Nishiyama Y., Abe S, & Sekimizu K. (2017). Lactic acid bacteria of the Leuconostoc genus with high innate immunity-stimulating activity. Drug discoveries & therapeutics, 11(1).

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