Total protein concentration in each sample was determined by a bicinchoninic acid colorimetric assay (Fisher Cat#23223 and 23224), using bovine serum albumin as a standard (Thermo Fisher Cat#23210). Protein was resolved on 5–20% gradient polyacrylamide gels using equal protein loading. Proteins were transferred to 0.2 um nitrocellulose membranes and detected with primary antibodies (Syn9027, CNDR, 1:20,000), pS129 α-synuclein (Abcam Cat#ab168381, 1:1000), GCase (Sigma-Aldrich Cat#G4171, 1:1,000), GCase (Santa Cruz Biotech Cat#sc-166407, 1:200), GFAP (Agilent Cat# Z0334, 1:5,000) or GAPDH (2-RGM2, Advanced Immunological, 1:5000). Primary antibodies were detected using IRDye 800 (Li-cor 925–32210) or IRDye 680 (Li-cor 925–68071) secondary antibodies, scanned on a Li-cor Odyssey Imaging System and analyzed using Image Studio software. Values obtained from this program were normalized to GAPDH or GFAP, and were further normalized to the mean control values.
Gcase
Manufactured by Merck Group
The GCase is a laboratory equipment product developed by Merck Group. It is designed to measure the activity of the enzyme glucocerebrosidase (GCase) in biological samples. The GCase plays a crucial role in the breakdown of glucocerebroside, a lipid molecule. The product provides researchers and scientists with a reliable tool to assess GCase activity, which is important for the study of various metabolic disorders and diseases.
Automatically generated - may contain errors
Lab products found in correlation
Ps129 α synuclein, by Abcam (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Odyssey imaging system, by LI COR (1 mentions)
Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions)
βiii tubulin, by BioLegend (1 mentions)
Calnexin, by Cell Signaling Technology (1 mentions)
Cathepsin d, by Santa Cruz Biotechnology (1 mentions)
Lamp1 sc 20011, by Santa Cruz Biotechnology (1 mentions)
Rab10, by Cell Signaling Technology (1 mentions)
α tubulin, by Merck Group (1 mentions)
Vps13c, by Proteintech (1 mentions)
Cathepsin b, by R&D Systems (1 mentions)
Gapdh, by Merck Group (1 mentions)
Tom20, by BD (1 mentions)
2 protocols using gcase
1
Western Blot Quantification of Neurological Proteins
2
Protein Analysis in Neurodegenerative Disorders
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Primary antibodies for western blotting were VPS13C (HPA043507, 1:500; Sigma-Aldrich, 28676-1-AP, 1:800; Proteintech), phosphoRab10-T73 (230261, 1:500; Abcam), Rab10 (8127, 1:1,000; Cell Signaling), βIII-Tubulin (4466S, 1:4,000; Biolegend), TH (657012, 1:2,000; Millipore), GAPDH (2,118, 1:2,000; Millipore), α-Tubulin (5168, 1:20,000; Sigma-Aldrich), LAMP2 (H4B4, 1:1,000; DSHB), GCase (G4171, 1:1,000; Sigma-Aldrich), Rab7 (137029, 1:1,000; Abcam), HA (3724, 1:2,000; Cell Signaling), Calnexin (1:1,000; Cell Signaling), TOM20 (612278, 1:1,000; BD Biosciences), PEX5 (83020S, 1:500, Cell Signaling), GFP (1544, 1:2,000; Sigma-Aldrich), Myc (2278, 1:2,000; Cell Signaling), cathepsin B (AF953, 1:2,000; R&D systems), cathepsin D (6487, 1:1,000; Santa Cruz), LRRK2 (ab133474, 1:500; Abcam), LRRK2-S935 (ab133450, 1:500; Abcam), EHBP1 (17637-1-AP, 1:1,000; Proteintech), and PPM1H (PA5-26102, 1:1,000; Invitrogen).
Primary antibodies for immunofluorescence were βIII-Tubulin (4466S, 1:300; Biolegends), TH (657012, 1:300; Millipore), and LAMP1 (sc-20011, 1:300; Santa Cruz).
Primary antibodies for PLA were VPS13C (28676-1-AP, 1:100; Proteintech) and Rab10 (ab104859, 1:100; Abcam).
Primary antibodies for immunofluorescence were βIII-Tubulin (4466S, 1:300; Biolegends), TH (657012, 1:300; Millipore), and LAMP1 (sc-20011, 1:300; Santa Cruz).
Primary antibodies for PLA were VPS13C (28676-1-AP, 1:100; Proteintech) and Rab10 (ab104859, 1:100; Abcam).
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