Human hepatoma HepG2 cells (three stocks with different lot numbers) were obtained from the Human Science Research Resources Bank (Osaka, Japan). Cells were cultured in Dulbecco's modified Eagle's medium low glucose (WAKO Pure Chemical Industries, Osaka, Japan) supplemented with 10% (v/v) fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA) in a humidified atmosphere of 5% CO 2 at 37°C. Cells from different stocks were cultured simultaneously and passaged to the same passage number (range 3-10). Prior to treatment, cells were grown in 60-mm culture dishes (AGC Techno Glass Co., Shizuoka, Japan) to 80%-90% confluence. β-Naphthoflavone was purchased from WAKO Pure Chemical Industries, and 0.1, 1, and 10 mM stock solutions of β-Naphthoflavone were prepared in dimethyl sulfoxide (DMSO, WAKO Pure Chemical Industries). The concentrations of β-Naphthoflavone were based on a previous report (Hanioka et al., 2006) . For experiments, HepG2 cells were treated with 0.1% (v/v) β-Naphthoflavone stock solution in culture medium for 3 days. Culture medium containing β-Naphthoflavone was refreshed every day.
β naphthoflavone
Manufactured by Fujifilm
Sourced in Japan
β-naphthoflavone is a chemical compound used in laboratory research applications. It serves as a potent aryl hydrocarbon receptor (AhR) agonist, a key function in experimental studies.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Fujifilm (2 mentions)
Cycloheximide (chx), by Fujifilm (1 mentions)
Mg132, by Abcam (1 mentions)
Acetic acid, by Fujifilm (1 mentions)
Dexamethasone (dex), by Fujifilm (1 mentions)
Methimazole, by Merck Group (1 mentions)
Acivicin, by Merck Group (1 mentions)
Isoniazid, by Merck Group (1 mentions)
Acetonitrile, by Fujifilm (1 mentions)
Methazolamide, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium low glucose, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
3 protocols using β naphthoflavone
1
HepG2 Cell Culture and β-Naphthoflavone Treatment
2
Preparation of Chemical Compounds for Cell Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
3-Methylcholanthrene (3MC), indole-3-carbinol (I3C), indole-3-acetate (IAA), cycloheximide (CHX), and β-naphthoflavone (βNF) was purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Indirubin was kindly provided by Dr. Tomonari Matsuda (Kyoto University, Kyoto, Japan). MG-132 was purchased from Abcam (Tokyo, Japan). All other chemicals and solvents used were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ligands were dissolved in dimethyl sulfoxide (DMSO) and added to media. The final concentration of DMSO was adjusted to 0.1% (v/v) in culture media.
3
Synthesis and Characterization of Methazolamide Conjugates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methazolamide, acivicin, isoniazid and methimazole were purchased from Sigma Chemicals (St. Louis, MO, USA). Dexamethasone, β-naphthoflavone, dimethylsulfoxide (DMSO), acetic acid and acetonitrile were obtained from Wako Pure Chemicals (Osaka, Japan). In quantitative studies, we employed purified Methazolamide. It was purified in our laboratory by HPLC as described in the next paragraph. Cysteine, cysteinylglycine, and glutathione conjugates of Methazolamide were prepared in our laboratory according to the method described in the preceding paper [27 (link)]. N-[3 (link)-Methyl-5-sulfo-1,3,4-thiadiazol-2(3H)-ylidene]acetamide (MSO), and N-(3-methyl-5-mercapto-Δ4-1,3,4-thiadiazol–2-yl)acetamide (MSH) were also synthesized in our laboratory [14 (link)]. Fig. (1
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!