Glutathione reduced form gsh

Doxorubicin hydrochloride and ferritin from equine spleen were purchased from Sigma Aldrich (Shanghai, China). Erastin and ferrostatin-1 were obtained from Selleck (Shanghai, China). Deferoxamine was obtained from Novartis (Shanghai, China), glutathione reduced form (GSH) was purchased from Tokyo Chemical Industry (Shanghai, China). Fe³⁺(FeCl₃·6H₂O) was obtained from Sinopharm Chemical Reagent Co., Ltd, Float-A-Lyzer G2 Dialysis Cassettes (MWCO 3.5 kDa) and Tube-O-DIALYZER™ Micro Dialysis System (MWCO 50 kDa) were purchased from Sangon Biotech (Shanghai, China). PD-10 desalting columns were obtained from GE Healthcare. C11-BODIPY was obtained from Thermo Fisher. The kits for Cell Counting Kit-8 (CCK-8), enhanced BCA protein assay, and reactive oxygen species assay, anti-actin, goat anti-rabbit IgG (H + L)-HRP conjugate and goat anti-mouse IgG (H + L)-HRP conjugate were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Anti-GPX4 was obtained from Abcam. Anti-GPX4 and anti-actin were used as primary antibodies. Goat anti-rabbit IgG (H + L)-HRP conjugate and goat anti-mouse IgG (H + L)-HRP were used as secondary antibodies. All obtained chemicals were used without further purification.

Laminin, poly‐ornithine, thyroid hormone (T3), sodium l‐ascorbyl‐2‐phosphate (As‐2P), paraformaldehyde (PFA), Bis(cyclohexanone)oxaldihydrazone (Cuprizone), Hoechst 33342, ascorbic acid, n‐acetylcysteine, resveratrol, poly‐d‐lysine, papain, l‐cysteine, insulin, transferrin, progesterone, putrescine, BSA, and 5‐Fluoro‐2′‐deoxyuridine were purchased from Sigma–Aldrich. Phloretin (PT), Vitamin E (α‐tocopherol) and Glutathione reduced form (GSH) were purchased from Tokyo Chemical Industry. l‐carnitine hydrochloride and Vitamin B1 (Thiamine hydrochloride) were purchased from TargetMol. Rat tail collagen, collagenase II and dispase II were purchased from Roche. EGF, bFGF, and PDGF‐AA were purchased from Peprotech.