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Incucyte s3

Manufactured by Thermo Fisher Scientific
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The IncuCyte S3 is a live-cell imaging system that enables real-time, automated, and long-term monitoring of cell cultures. It provides quantitative data on cell proliferation, migration, and morphology without the need for cell labeling.

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Lab products found in correlation

Propidium iodide, by Thermo Fisher Scientific (1 mentions) Ionomycin, by Bio-Techne (1 mentions) Cytochalasin d, by Merck Group (1 mentions) Phrodo red e coli bioparticles, by Thermo Fisher Scientific (1 mentions) Yo pro 1 iodide, by Thermo Fisher Scientific (1 mentions)

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IncuCyte (3 citations)

3 protocols using incucyte s3

1

Microscopic Evaluation of Cell Viability

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Cells were treated with dimethylsulfoxide (DMSO) or 7.5 μM ionomycin (Tocris Bioscience) in complete media, unless otherwise indicated. Cells were visualized by live phase-contrast microscopy using the IncuCyte S3 system as described above while in culture for 48 hours. At the end of the time course, cells were treated with media containing propidium iodide (Thermo Fisher Scientific), and cells were again visualized by epifluorescence microscopy using IncuCyte S3. Morphometric analysis was performed in Neurotrack and Basic Analyzer software (Sartorius Corporation) as described above.
Wadhwani A.R., Affaneh A., Van Gulden S, & Kessler J.A. (2019). Neuronal Apolipoprotein E4 Increases Cell Death and Phosphorylated Tau Release in Alzheimer Disease. Annals of neurology, 85(5), 726-739.
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2

Phagocytic Assay for Microglia

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For the Phagocytic assay, 15,000 cells were seeded in a 96-well flat bottom plate in DMEM (4.5 g/L) or maturation medium, for mouse or iMGL, respectively. The cells were incubated for 24 h at 37 °C and 5% CO2. 4 h before treatment, the plate was placed inside the IncuCyte S3 to obtain a blank measurement of the cells. The cells were treated with Cytochalasin D (Sigma Aldrich, C8273, 10 μM) in microglia medium with pHrodo red E.coli bioparticles 1 ug/0.1 ml (Thermo Fischer, p35361) and monitored for a minimum of 24 h. The data was obtained using the IncuCyte S3.
Sabogal-Guáqueta A.M., Marmolejo-Garza A., Trombetta-Lima M., Oun A., Hunneman J., Chen T., Koistinaho J., Lehtonen S., Kortholt A., Wolters J.C., Bakker B.M., Eggen B.J., Boddeke E, & Dolga A. (2023). Species-specific metabolic reprogramming in human and mouse microglia during inflammatory pathway induction. Nature Communications, 14, 6454.
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3

Radiosensitivity of MCF7 Cells

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MCF7 cells were seeded in 6-well plates with 3 × 104 cells per well, allowed to attach, then treated with DMSO, NU-2 (0.5 μM), NU-1 (0.5 μM) or chrolactomycin (0.5 μM) and irradiated with 0 or 6 Gy. 7 days after IR, cells were incubated with 1 μM YO-PRO-1 iodide (Thermo Fisher Scientific) for 30 min at 37° C and imaged in the IncuCyteS3. Phase contrast and green channel images were acquired at 20X magnification. 25 non-overlapping fields were captured for each well. Quantitative analysis of cell confluency was performed using IncuCyteS3 2019 software.
Liu Y., Betori R.C., Pagacz J., Frost G.B., Efimova E.V., Wu D., Wolfgeher D.J., Bryan T.M., Cohen S.B., Scheidt K.A, & Kron S.J. (2022). Targeting telomerase reverse transcriptase with the covalent inhibitor NU-1 confers immunogenic radiation sensitization. Cell chemical biology, 29(10), 1517-1531.e7.
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