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17 plex mouse procartaplex panel

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The 17-Plex Mouse ProcartaPlex™ Panel is a multiplex assay designed to simultaneously measure the concentrations of 17 different analytes in mouse samples. The panel allows for the quantification of multiple cytokines, chemokines, and other protein targets in a single experiment.

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Lab products found in correlation

Luminex 200 system, by Merck Group (3 mentions) Mouse il 2 elisa, by Abcam (2 mentions) Cytokine bead array, by BD (2 mentions) Bio plex 200, by Bio-Rad (2 mentions) Procartaplex analyst software, by Thermo Fisher Scientific (1 mentions) Procartaplex 11plex, by Thermo Fisher Scientific (1 mentions) Hepes, by Corning (1 mentions) Gentamicin solution, by Merck Group (1 mentions) Soluble α cd3, by BD (1 mentions) L glutamine, by Corning (1 mentions) αcd28, by BD (1 mentions) Luminex 200, by Merck Group (1 mentions) Mouse tnf α and il 6 elisa kits, by BD (1 mentions) Direct camp elisa kit, by Enzo Life Sciences (1 mentions) 96 well cell culture plate, by Mabtech (1 mentions) Mouse tslp elisa max deluxe set, by BioLegend (1 mentions) Tissuelyser 2, by Qiagen (1 mentions) Bca protein assay kit, by Solarbio (1 mentions) Bio plex 200 system, by Bio-Rad (1 mentions) Halt protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions)

16 protocols using 17 plex mouse procartaplex panel

1

Cytokine Profiling of MOG-Stimulated Splenocytes

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Cytokine secretion was determined in the supernatants of MOG40-55 stimulated splenocytes using the Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex Panel (EPX170-26,087–901, Invitrogen) and TGF beta 1 Mouse Procartaplex Simplex Kit (EPX01A-20608–901, Invitrogen), according to the manufacturer’s instructions. Data were analyzed with a Magpix instrument (Luminex Corporation, Austin, TX, USA) and ProcartaPlex Analyst software (Thermo Fisher Scientific).
Edo Á., Calvo-Barreiro L., Eixarch H., Bosch A., Chillón M, & Espejo C. (2022). Therapeutic Effect of IL-21 Blockage by Gene Therapy in Experimental Autoimmune Encephalomyelitis. Neurotherapeutics, 19(5), 1617-1633.
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2

Profiling Cytokine Responses in Murine Lung Infection

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Lung tissues and plasma of mice were collected after challenge with PBS or 108 CFU of M. catarrhalis (73-OR or 35-OR). The levels of Ifng, Il12p70, Il13, Il1b, Il2, Il4, Il5, Il6, Il18, Il10, Il17a, Il22, Il23, Il27, Il9, Tnf, and Csf2 were measured using the Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex™ Panel (Invitrogen, Thermo Fisher Scientific, USA).
Liu Y.L., Ding R., Jia X.M., Huang J.J., Yu S., Chan H.T., Li W., Mao L.L., Zhang L., Zhang X.Y., Wu W., Ni A.P, & Xu Y.C. (2022). Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells. Emerging Microbes & Infections, 11(1), 2055-2068.
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3

Multiplex Cytokine Profiling

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Skin tissue lysis mixtures and Raw264.7 cells supernatant were collected and assessed by 17-Plex Mouse ProcartaPlex™ Panel (EPX170-26087-901, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. The data were then read using a Luminex 200 analyzer. The data were fitted to the standard curve by the five-parameter nonlinear regression method, and the concentration value was calculated.
Wang Y., Qi C., Feng F., Hu X., Zhao N., Zhao J., Di T., Meng Y., Yang D., Zhu H., Zhang X., Li P, & Wang Y. (2023). Resveratrol Ameliorates Imiquimod-Induced Psoriasis-Like Mouse Model via Reducing Macrophage Infiltration and Inhibiting Glycolysis. Journal of Inflammation Research, 16, 3823-3836.
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4

Cytokine Profiling in PBMC and Spinal Cord

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In PBMC co-culture, ELISA assays for prostaglandin E2 (pGE2, Abcam cat. no.: ab133021), tumor necrosis factor alpha-inducible protein 6 (TSG-6, Sigma-Aldrich, cat. no.: RAB1092–1KT), and TGFβ1 (Invitrogen, cat. no.: 88-8350-22) were performed per the manufacturer’s protocols. Additionally, latent TGFβ1 was activated by acidification of the cell culture supernatants, based on manufacturer instructions. Ineffective acidification led to negative absorbance values in several wells in TGFβ1 ELISA assay which were eliminated from the presented data. For the Luminex assay, 50 μL of PBMC culture supernatants were collected and either frozen at −80 °C or immediately analyzed using a human custom ProcartaPlex (11plex, ThermoFisher Scientific, Vienna, Austria) with Luminex MAGPIX. Results were then reported as mean fluorescence intensity.
To measure inflammatory cytokines in spinal cords, the supernatant after cell isolations of spinal cords was harvested. Supernatant solutions (100 μL) were then immediately kept in −80 °C and thawed immediately before performing the Luminex assay. Thawed samples were centrifuged at 10,000 × g for 5 min to extract cells and debris from the solution. We then performed a Luminex assay using Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex Panel (ThermoFisher Scientific, Vienna, Austria).
Riazifar M., Mohammadi M.R., Pone E.J., Yeri A., Lässer C., Segaliny A.I., McIntyre L.L., Shelke G.V., Hutchins E., Hamamoto A., Calle E.N., Crescitelli R., Liao W., Pham V., Yin Y., Jayaraman J., Lakey J.R., Walsh C.M., Van Keuren-Jensen K., Lotvall J, & Zhao W. (2019). Stem Cell-Derived Exosomes as Nanotherapeutics for Autoimmune and Neurodegenerative Disorders. ACS nano, 13(6), 6670-6688.
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5

Multiplex Cytokine Profiling Post-HCT

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The level of cytokines in the serum collected at day 28 post-HCT was measured using a multiplex assay (Luminex, Life-Technologies©) with the Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex™ Panel (ThermoFisher) following manufacturer’s instructions.
Alvarez M., Pierini A., Simonetta F., Baker J., Maas-Bauer K., Hirai T, & Negrin R.S. (2021). Infusion of Host-Derived Unlicensed NK Cells Improves Donor Engraftment in Non-Myeloablative Allogeneic Hematopoietic Cell Transplantation. Frontiers in Immunology, 11, 614250.
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6

Cytokine Profiling of Activated Lymph Cells

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Lymph node cells (5 × 105) were seeded into 96-well u-bottom plates with soluble α-CD3 (BD Pharmingen) (2 μg/ml) and α-CD28 (BD Pharmingen) (2 μg/ml) in RPMI media containing L-glutamine and HEPES (Cellgro) with 10% fetal bovine serum (Hyclone), and Gentamicin solution (Sigma-Aldrich) (cRPMI). Cells were incubated for 24h in a humidified incubator (37°C/5% CO2). Cytokines in the supernatants were measured using the Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex™ Panel (ThermoFisher) according to manufacturer’s instructions and data was acquired using a Luminex 200 system (Millipore).
Shane H.L., Lukomska E, & Anderson S.E. (2019). Topical application of the quaternary ammonium compound didecyldimethylammonium chloride activates type 2 innate lymphoid cells and initiates a mixed-type allergic response. Toxicological sciences : an official journal of the Society of Toxicology, 168(2), 508-518.
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7

Quantification of Inflammatory Cytokines

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Serum inflammatory cytokines were quantified by the Luminex 200 instrument (Merck Millipore, Billerica, MA, USA) using a Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex™ Panel (Thermo Fisher Scientific). Cytokines in RAW264.7 cell supernatants were analyzed by mouse TNF-α and IL-6 ELISA kits (BD Biosciences, San Diego, CA, USA) as described previously [16] (link). For cAMP detection, cells and psoriatic skin biopsies were lysed and isolated by 0.1 M HCl and the intracellular cAMP level was quantified by using the direct cAMP ELISA kits (Enzo Life Sciences, The Netherlands) according to the directions.
Li H., Zhang X., Xiang C., Feng C., Fan C., Liu M., Lu H., Su H., Zhou Y., Qi Q., Xu Y, & Tang W. (2021). Identification of phosphodiesterase-4 as the therapeutic target of arctigenin in alleviating psoriatic skin inflammation. Journal of Advanced Research, 33, 241-251.
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8

Cytokine Profiling in Tuberculosis Infection

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The mice in each group were killed at week 29 after M. tuberculosis infection, and the spleens were obtained to prepare splenocyte suspensions. A 100-μL volume of splenocytes at the concentration of 3 × 106/mL was added to the well of a 96-well cell culture plate (Mabtech), followed by incubation with 50 μL of PBS (negative control), 50 μL of Th1 peptide (100 μg/mL), 50 μL of CTL peptide (100 μg/mL), 50 μL of peptide pool (100 μg/mL), or 50 μL of PHA (positive control, 50 μg/mL) at 37°C for 48 h. Then, after centrifugation at 500 × g for 10 min, the supernatant of each sample was collected to detect the levels of Th1/Th2/Th9/Th17/Th22/Treg cytokines (IFN-γ, IL-12p70, IL-13, IL-1β, IL-2, IL-4, IL-5, IL-6, TNF-α, GM-CSF, IL-18, IL-10, L-17A, IL-22, IL-23, IL-27, and IL-9) by using a Th1/Th2/Th9/Th17/Th22/Treg cytokine 17-Plex mouse ProcartaPlex panel (catalog no. EPX170-26087-901; Thermo Fisher, China) according to the manufacturer’s instructions.
Gong W., Liang Y., Wang J., Liu Y., Xue Y., Mi J., Li P., Wang X., Wang L, & Wu X. (2022). Prediction of Th1 and Cytotoxic T Lymphocyte Epitopes of Mycobacterium tuberculosis and Evaluation of Their Potential in the Diagnosis of Tuberculosis in a Mouse Model and in Humans. Microbiology Spectrum, 10(4), e01438-22.
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9

Multiplex Cytokine Analysis in Mouse Ears

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Ears (1 per mouse) were mechanically disrupted on a TissueLyser II (Qiagen) in 0.75 ml T-PER protein extraction reagent (Pierce) and soluble proteins were quantified by BCA protein assay (Pierce). Cytokines were measured using the Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex™ Panel (ThermoFisher) according to manufacturer’s instructions and data was acquired using a Luminex 200 system (Millipore). TSLP, IL-4, and IL-5 were assessed by ELISA according to manufacturers’ instructions using Mouse TSLP ELISA MAX Deluxe™ Set (BioLegend), Mouse IL-4 ELISA Ready-SET-Go! (eBioscience), and BD OptEIA™ Set Mouse IL-5 (BD Biosciences), respectively.
Shane H.L., Lukomska E, & Anderson S.E. (2019). Topical application of the quaternary ammonium compound didecyldimethylammonium chloride activates type 2 innate lymphoid cells and initiates a mixed-type allergic response. Toxicological sciences : an official journal of the Society of Toxicology, 168(2), 508-518.
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10

Cytokine Profiling in Colon Tissue

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Firstly, we selected all colon sections near anus. As reported in the previous study,49 (link) colons were cut into small parts and PBS with protease inhibitor (Solarbio, China) was added following thawing and weighing, then homogenization was conducted on ice followed by centrifugation (5 min at 13,000 rpm). The supernatant was then stored at −80°C until analysis. Next, the supernatant was normalized using BCA protein assay kits (Solarbio, China) according to the manufacturer’s instructions. TNF-α, IL-6, IL-1β, IL-18, IL-22, IL-9, IL-10, IL-4, IL-5, IFN-γ and IL-17A were measured for cytokine profiling assay using the Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex™ Panel (EPX170-26087-901, Thermo Fisher Scientific). Then the 17 mouse cytokine levels were determined using Bio-Plex 200 System (Bio-Rad Laboratories).
Fan L., Qi Y., Qu S., Chen X., Li A., Hendi M., Xu C., Wang L., Hou T., Si J, & Chen S. (2021). B. adolescentis ameliorates chronic colitis by regulating Treg/Th2 response and gut microbiota remodeling. Gut Microbes, 13(1), 1826746.
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