Mpw 351 r

For analysis of lipid peroxidation, MDA leaf material (0.5 g) was homogenized using 5% trichloracetic acid (TCA) (Sigma-Aldrich). The method of Hodges et al. with slight modifications was used to estimate MDA. The homogenates were centrifuged at 13 g for 17 min (centrifuge MPW-351 R) and the supernatant was added to 20% TCA containing 0.5% thiobarbituric acid (TBA) (Alfa Aesar). The homogenate was incubated in a heater at 95 °C for 30 min (Blockthermostat BT 200, Kleinfeld, Labortechnik) and subsequently cooled on ice. The optical density was measured at 532 and 660 nm using a spectrophotometer (Analytik Jena Specord 210 Plus, Analytik Jena, Jena, Germany)). The results were expressed in µmol g⁻¹ FW [63 (link)].

For analysis of MDA and H₂O₂, leaf material (0.5 g) was homogenized using 5% trichloracetic acid (TCA) (Sigma-Aldrich, St. Louis, MO, USA). The method of Hodges et al. [72 (link)] with slight modifications, have been used to estimate MDA. The homogenates were centrifuged at 12.13× g for 17 min (centrifuge MPW-351 R), and supernatant was added to 20% TCA containing 0.50% thiobarbituric acid (TBA) (Alfa Aesar, Haverhill, MA, USA). The homogenate was incubated in a heater at 95 °C for 30 min (Blockthermostat BT 200) and then subsequently cooled on the ice. The optical density was measured at 532 and 660 nm by spectrophotometer (Analytik Jena Specord 210 Plus, Analytik Jena, Jena, Germany). The results were expressed in µmol g⁻¹ FW [72 (link)]. To avoid desiccation effects, the RWC was used as a factor to calculate the MDA content and to estimate the effective differences between treatments.