Saccharomyces cerevisiae strain AH109, control vectors pGADT7, pGBKT7, pGADT7-T, pGBKT7-lam, and pGBKT7-p53 were purchased from Clontech (Mountain View, CA, USA). Yeast strain AH109 was co-transformed with the combination of the pGADT7 and the pGBKT7 plasmids. Transformed yeast cells containing both plasmids were first grown on SD-minus Trp/Leu plates (DDO) to maintain the two plasmids and then were sub-cloned replica plated on SD-minus Trp/Leu/Ade/His plate (QDO).
Pgbkt7 p53
Manufactured by Takara Bio
Sourced in United States
The PGBKT7-p53 is a plasmid vector designed for the expression of the p53 tumor suppressor protein in bacterial and yeast systems. It contains the p53 gene, as well as elements necessary for plasmid replication and selection. The core function of this product is to provide a tool for the study and manipulation of the p53 protein in controlled experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Pgadt7 t, by Takara Bio (6 mentions)
Pgbkt7 lam, by Takara Bio (6 mentions)
Pgadt7, by Takara Bio (5 mentions)
Pgbkt7, by Takara Bio (5 mentions)
Matchmaker gal4 based two hybrid system 3, by Takara Bio (1 mentions)
3 amino 1 2 4 triazole, by Merck Group (1 mentions)
Pact2, by Takara Bio (1 mentions)
Pcdna3.1 expression plasmid, by Thermo Fisher Scientific (1 mentions)
Pgem t easy vector, by Promega (1 mentions)
7 protocols using pgbkt7 p53
1
Yeast Two-Hybrid Screening Protocol
2
Yeast Two-Hybrid Protein Interactions
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Protein interactions were analyzed using GAL4 fusion proteins in a yeast two-hybrid system. Saccharomyces cerevisiae strain AH109 and control vectors pGADT7, pGADT7-T, pGBKT7-p53, and pGBKT7-Lam were purchased from Clontech. The AH109 yeast strain was transformed with the bait plasmid pGBK-UL80.5 (in fusion with GAL4-BD) and subsequently transformed with selected expression clones pGAD-UBC9 and UBC9 deletion mutant series (in fusion with GAL4-AD). Positive clones were selected on a synthetic dropout medium that lacked four nutrients, including tryptophan, leucine, adenine, and histidine (QDO), and were tested for β-galactosidase activity.
3
Yeast Two-Hybrid Assay for C4 and CLV1 Interaction
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The CDS of C4 was cloned into pGBKT7 and the intracellular catalytic domain of CLV1 (704AA-967AA) was cloned into pGADT7. Yeast two-hybrid (Y2H) assays were performed using the Matchmaker GAL4-based Two-Hybrid System 3 (Clontech) according to the manufacturer’s instructions. Interactions were tested by stringent selection (SD/–Leu/–Trp/–His) supplied with 1 mM 3-amino-1,2,4-triazole (Sigma). Yeast cells with pGBKT7-p53 and pGADT7-T (Clontech) were used as positive controls; the yeast cells with pGBKT7-Lam and pGADT7-T (Clontech) were used as negative controls.
4
Yeast Two-Hybrid Screening of DENV Proteins
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Human MMP-9 gene was subcloned in pGBKT7, and DENV NS1, E, and NS4A were subcloned in pGADT7 respectively. control vectors pGBKT7, pGADT7, pGBKT7-p53, pGADT7-T, and pGBKT7-lam, and some reagents were purchased from Clontech Laboratories. All experimental procedures were done following the Matchmaker Gold Yeast 2-Hybrid System User Manual. Briefly, yeast strain AH109 cells were co-transformed with plasmid pGADT7 and plasmid pGBKT7. Transformed yeast cells were grown in SD-minus Trp/Leu plates (DDO) for 24–48 h, and then subcloned replica plated on SD-minus Trp/Leu/Ade/His plate (QDO) for another 48–96 h.
5
Yeast Two-Hybrid Screening Protocol
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Saccharomyces cerevisiae strain AH109 and control vectors pGADT7, pGBKT7, pGADT7-T, pGBKT7-lam, and pGBKT7-p53 were purchased from Clontech (Mountain View, CA, USA). Yeast strain AH109 was co-transformed with the combination of the pGADT7 and the pGBKT7 plasmids. Transformed yeast cells containing both plasmids were first grown on SD-minus Trp/Leu plates (DDO) to maintain the two plasmids and then were subcloned replica plated on SD-minus Trp/Leu/Ade/His plate (QDO).
6
Yeast Two-Hybrid System Protocol
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Saccharomyces cerevisiae strain AH109 and control vectors pGADT7, pGBKT7, pGADT7-T, pGBKT7-Lam, and pGBKT7-p53 were purchased from Clontech (Mountain View, CA, USA). Yeast strain AH109 was co-transformed with the combination of the pGADT7 and the pGBKT7 plasmids. Transformed yeast cells containing both plasmids were first grown on SD-minus Trp/Leu plates (DDO) to maintain the two plasmids and then were sub-cloned replica plated on SD-minus Trp/Leu/Ade/His plate (QDO).
7
Molecular Cloning and Plasmid Construction
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The parental pCS2+ vector was kindly provided by Sergei Tevosian (Dartmouth Medical School, Hanover, NH). pCS2+FOG-2 was kindly provided by Alan Cantor (Children’s Hospital, Boston, Massachusetts). pcDNA3-Nkx2.5 was kindly provided by Mona Nemer (University of Ottawa, Canada). Human GATA-4, Art27 and Art27/GAL(DBD) were cloned into the pCS2+ expression plasmid. The BNP, ANP and αMHC luciferase reporter constructs, GATA-1 and GATA-6 were cloned into the pcDNA3.1 expression plasmid (Invitrogen). FOG-2 and Art27 GST fusions were created by subcloning into pDEST15 (Gateway Technology, Invitrogen). FOG-2, FOG-2 (856–1156), GATA-4, GATA-4 1–216, GATA-4 217–440, GATA-4 N-finger and GATA-4 C-finger were created by subcloning into pGBKT7 (Clontech). Art27 was subcloned into pACT2 (Clontech). pGBKT7-p53, pGADT7-T antigen, and pGBKT7-Lamin C were purchased from Clontech. pGEM-T:easy vector was purchased from Promega.
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