Goat anti murine tyrosinase igg antibody

For the present study, the compound, mushroom tyrosinase (lyophilized powder ≥ 1000 units/mg solid), and protein kinase inhibitors, protein kinase A (PKA) inhibitor (H89), protein kinase B (PKB) inhibitor (LY294002), protein kinase C (PKC) inhibitor (Ro-32-0432), and MEK1 inhibitor (PD98059) were purchased from Sigma-Aldrich, St. Louis, Missouri, United States. Goat anti-murine tyrosinase IgG antibody and Alexa Fluor® 594 donkey anti-goat IgG (H+L) (2 mg/mL) were purchased from Life Technologies, North America, United States. Dulbecco's Modified Eagle Medium (AT006A-5L), fetal bovine serum (RM10432-100 mL), Antibiotic Antimycotic Solution 100x (A002-20 mL), Trypsin-EDTA Solution 1x (TCL042-5 ×  100 mL), MTT {[3-(4,5-dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide} (TC191-500MG), 4′,6-diamidino-2-phenylindole (DAPI) (TC229-5MG), phosphate buffered saline (RM7385-1PK), and Trypan blue, Certified (RM263-5G), were purchased from HiMedia Laboratories Pvt. Ltd., Mumbai.

For the present study, the compound, mushroom tyrosinase (lyophilized powder ≥1000 unit/mg solid), was purchased from Sigma-aldrich St. Louis, Missouri, United States. Goat anti-murine tyrosinase IgG antibody and Alexa Flour ® 594 dunkey anti-goat IgG (H+L) (2mg /mL) was purchased from life technologies North America, United States. Dulbecco’s Modified Eagle Medium (AT006A-5L) Fetal bovine serum (RM10432-100ML), Antibiotic Antimycotic Solution 100X (A002-20ML), Trypsin-EDTA solution1X (TCL042-5×100ML), 4', 6-diamidino-2-phenylindole (DAPI) (TC229-5MG), Phosphate buffered saline (RM7385-1PK) and Trypan blue, Certified (RM263-5G) were purchased from HiMedia Laboratories Pvt.Ltd. Mumbai.