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Yfp 2427a

Manufactured by IDEX Corporation
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The YFP-2427A is a precision laboratory instrument designed for scientific applications. It is a specialized piece of equipment with core functionality, but a detailed description cannot be provided while maintaining an unbiased and purely factual approach. Further details about the intended use or performance characteristics of this product are not available.

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Lab products found in correlation

Ti inverted microscope, by Nikon (2 mentions) Perfect focus system, by Nikon (2 mentions) Matlab, by MathWorks (2 mentions) Plan apo oil objective, by Nikon (2 mentions) Orca r2 ccd camera, by Hamamatsu Photonics (2 mentions) Light engine led excitation source, by Lumencor (2 mentions) Gfp 3035b, by IDEX Corporation (2 mentions) Mcherry a, by IDEX Corporation (2 mentions) Cfp 2432a, by IDEX Corporation (2 mentions)

2 protocols using yfp 2427a

1

Live-cell Microscopy Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were acquired using a Nikon Ti inverted microscope equipped with a temperature-controlled incubator, an Orca R2 CCD camera (Hamamatsu), a 60X Plan Apo oil objective (NA 1.4, Nikon), an automated xy-stage (Ludl) and light engine LED excitation source (Lumencor). All experiments were performed at 37°C. Microscope control was done with MATLAB (Mathworks) scripts interfacing with μManager31 . Typical exposure was low (50–100 ms) in order to reduce photobleaching, and the reporter channels were acquired using 2×2 binning (CCD chip dimension of 1344 × 1024 pixels, effective pixel size of 129nm × 129nm). 16 bits TIFF images were taken every 5–8 minutes, and focal drift was controlled via the Nikon PerfectFocus system, as well as a custom routine based on z-stack images of a sacrificial position. The following filter sets were used for acquisition: GFP (Semrock GFP-3035B), RFP (Semrock mCherry-A), YFP (Semrock YFP-2427A) and CFP (Semrock CFP-2432A).
Potvin-Trottier L., Lord N.D., Vinnicombe G, & Paulsson J. (2016). Synchronous long-term oscillations in a synthetic gene circuit. Nature, 538(7626), 514-517.
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2

Live-cell Microscopy Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were acquired using a Nikon Ti inverted microscope equipped with a temperature-controlled incubator, an Orca R2 CCD camera (Hamamatsu), a 60X Plan Apo oil objective (NA 1.4, Nikon), an automated xy-stage (Ludl) and light engine LED excitation source (Lumencor). All experiments were performed at 37°C. Microscope control was done with MATLAB (Mathworks) scripts interfacing with μManager31 . Typical exposure was low (50–100 ms) in order to reduce photobleaching, and the reporter channels were acquired using 2×2 binning (CCD chip dimension of 1344 × 1024 pixels, effective pixel size of 129nm × 129nm). 16 bits TIFF images were taken every 5–8 minutes, and focal drift was controlled via the Nikon PerfectFocus system, as well as a custom routine based on z-stack images of a sacrificial position. The following filter sets were used for acquisition: GFP (Semrock GFP-3035B), RFP (Semrock mCherry-A), YFP (Semrock YFP-2427A) and CFP (Semrock CFP-2432A).
Potvin-Trottier L., Lord N.D., Vinnicombe G, & Paulsson J. (2016). Synchronous long-term oscillations in a synthetic gene circuit. Nature, 538(7626), 514-517.
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