Cell titer glow assay

Manufactured by Promega

The Cell Titer Glow assay is a luminescent cell viability assay that measures the quantification of ATP as an indicator of metabolically active cells. The assay utilizes a thermostable luciferase that generates a stable luminescent signal proportional to the amount of ATP present in the sample.

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Lab products found in correlation

Decitabine, by Merck Group (1 mentions) Go 203, by Merck Group (1 mentions)

Spelling variants of this product

Cell Titer Glow (37 citations) Cell titer Glow assay reagent (2 citations)

3 protocols using cell titer glow assay

Synergistic Effects of GO-203 and Decitabine

Cited 1 time

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Cells were seeded in a 96-well plate and treated with GO-203 [47 ] and/or decitabine (Sigma). Cell viability was assessed using cell titer glow assay (Promega). Combination index values were determined by isobologram analysis using CompuSyn software. Combination index values < 1 were considered as synergistic and > 1 as antagonistic [44 (link)].

Tagde A., Rajabi H., Stroopinsky D., Gali R., Alam M., Bouillez A., Kharbanda S., Stone R., Avigan D, & Kufe D. (2016). MUC1-C induces DNA methyltransferase 1 and represses tumor suppressor genes in acute myeloid leukemia. Oncotarget, 7(26), 38974-38987.

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Butyrate-induced ATP Levels in GLUTag Cells

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ATP measurements on GLUTag cells in response to 1 mM butyrate were performed in the same culture conditions as GLP-1 secretion assays, using Cell Titer Glow assay (Promega) according to the manufacturer’s protocol. Luciferase activity was measured using Victor luminescence counter (PerkinElmer). Relative ATP levels were calculated from the measured luminescence setting the control condition (DMSO/control) at 1.

Ducastel S., Touche V., Trabelsi M.S., Boulinguiez A., Butruille L., Nawrot M., Peschard S., Chávez-Talavera O., Dorchies E., Vallez E., Annicotte J.S., Lancel S., Briand O., Bantubungi K., Caron S., Bindels L.B., Delzenne N.M., Tailleux A., Staels B, & Lestavel S. (2020). The nuclear receptor FXR inhibits Glucagon-Like Peptide-1 secretion in response to microbiota-derived Short-Chain Fatty Acids. Scientific Reports, 10, 174.

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Evaluating Cytotoxic Effects on Leukemia Cell Lines

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MV4-11, MOLM-13, K562, REH, MOLT-4 (all from ATCC) and KOPN-8 (from DSMZ) human leukemia cell lines were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and penicillin/ streptomycin. Cells were plated at a density of 1.0 x 10⁶ cells per ml in 96-well plates. SPK111 and the control peptide SPK110 were dissolved in DMSO and added to the cells at the indicated concentrations. 24 h later cell viability was measured using the Cell Titer Glow assay (Promega) and reported as a percentage of DMSO (vehicle) treated cells.

Barretto N.N., Karahalios D.S., You D, & Hemenway C.S. (2014). An AF9/ENL-targted peptide with therapeutic potential in mixed lineage leukemias. Journal of experimental therapeutics & oncology, 10(4), 293-300.

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