Seven peptides QITEGEDGGG, ITEGEDGGG, QITEGED, QEGEDGGG, QITEGGGG, HIQKEDVPSER, and HIQKVPSER were synthesized by SciLight Biotechnology Ltd. (Beijing, China) based on the literature [13 (link),14 (link)]. Virgin walnut oil was provided by Xinmu Biotechnology Ltd. (Beijing, China). Linoleic acid, linolenic acid, arachidonic acid and oleic acid were purchased from J&K Chemical (Hebei, China). Nt-butyl-α-phenylnitrone (PBN) was obtained from Sigma-Aldrich (St. Louis, MO, USA). C11-BODIPY 581/591 was purchased from Thermo Fisher Scientific (Waltham, MA, USA) and fluorescein isothiocyanate (FITC) was purchased from Lablead Biotech (Beijing, China).
Oleic acid
Manufactured by J&K Scientific
Sourced in China
Oleic acid is a naturally occurring long-chain monounsaturated fatty acid. It is a colorless to pale yellow oily liquid with a mild odor. Oleic acid is commonly used as a laboratory reagent and in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Linoleic acid, by J&K Scientific (2 mentions)
Linolenic acid, by J&K Scientific (2 mentions)
Oleylamine, by J&K Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Bodipy 581 591 c11, by Thermo Fisher Scientific (1 mentions)
Nickel chloride hexahydrate, by Sinopharm (1 mentions)
Dopamine, by Thermo Fisher Scientific (1 mentions)
Iron 2 chloride tetrahydrate, by Sinopharm (1 mentions)
Anhydrous ethanol, by Sinopharm (1 mentions)
Iron 3 chloride hexahydrate, by Sinopharm (1 mentions)
Ammonium hydroxide, by Sinopharm (1 mentions)
Sodium hydroxide (naoh), by Sinopharm (1 mentions)
Cetyl trimethyl ammonium bromide, by J&K Scientific (1 mentions)
Fluorescein diacetate, by Merck Group (1 mentions)
Haucl4, by J&K Scientific (1 mentions)
Nabh4, by J&K Scientific (1 mentions)
L ascorbic acid, by J&K Scientific (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
7 protocols using oleic acid
1
Peptide Synthesis and Lipid Analysis
2
Synthesis and Characterization of Magnetic Nanoparticles
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Dopamine was purchased from Acros Organic. Octadecylamine (ODA) and tris (hydroxymethyl) aminomethane (Tris) were obtained from Shanghai Chemical Regent Co., Ltd (China). Iron (III) chloride hexahydrate (FeCl3·6H2O, 98%), iron (II) chloride tetrahydrate (FeCl2·4H2O, 98%), ammonium hydroxide (NH3·H2O, 28–30%), anhydrous ethanol (AR), sodium hydroxide (NaOH, AR), dimethylglyoxime (DMG) and nickel chloride hexahydrate (NiCl2·6H2O, 98%) were purchased from Sinopharm Chemical Reagent Co., Ltd (China). Oleic acid (OA, 99%) was purchased from J&K Chemical Ltd. The p-aminobenzene sulfonic acid (C6H7NO3S, AR) and alpha naphthylamine (α-C10H9N, AR) were obtained from Macklin Biochemical Co., Ltd. Sodium bicarbonate (NaHCO3, AR) and acetic acid (Ac, AR) were produced from Shanghai Aladdin Biochemical Technology Co., Ltd. All the chemical agents were used as obtained.
3
Synthesis of Multicomponent Nanoparticles
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Iron(III) acetylacetonate (Fe(acac)3), 1,2-hexadecanediol, oleylamine, polyethylene glycol (PEG), oleic acid, benzyl ether, cetyl trimethyl ammonium bromide (CTAB), HAuCl4, NaBH4, l -ascorbic acid, agar and doxorubicin (DOX) were purchased from J&K Scientific Ltd. Glycolide and Lactide were purchased from Purac (Netherlands) and further purified by recrystallization from ethyl acetate for several times. Stannous octoate, polyvinyl alcohol (PVA) and fluorescein diacetate were purchased from Sigma Aldrich. Fast membrane emulsification equipment (FMEM-500 M) and Shirasu porous glass (SPG) membrane were purchased from National Engineering Research Center for Biotechnology (Beijing). The glass membranes were annular cylinders (inner diameter = 8 mm, external diameter = 10 mm, length = 170 mm) with pore sizes of 7.2 μm. All other reagents were purchased from Beijing Chemical Reagents Company and used as received without further purification. Cells were supplied by China Infrastructure of Cell Line Resources.
4
Quantification of Unsaturated Fatty Acids
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Standard UFAs including linolenic acid (99.0%), linoleic acid (97.0%), and oleic acid (99.1%) (J&K Scientific) were dissolved in DMSO by ultrasonic to prepare standard samples at various concentrations of 0, 20, 40, 50, 60, 80, and 100 µg/ml for all three standard UFAs. Twenty micro liters of each standard sample was chromatographied on an Agilent RRLC system under same conditions described as above. Signal peak area was linearly regressed with the concentration of standard unsaturated fatty acid to make the standard curve for each unsaturated fatty acid. Same volume (20 µl) of CCS‐HE sample was chromatographied as same as standard UFAs on an Agilent RRLC system, and the area of identical signal peak to each standard unsaturated fatty acid was measured with software on Agilent RRLC system. Individual unsaturated fatty acid content and the ratio in mass of three UFAs were calculated according to the prepared standard curves.
5
Synthesis of Multi-Arm PEG Hydrogel
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4-Armed poly(ethylene glycol) succinimidyl (Tetra-PEG-NHS, Mw = 20 kDa, Mw/Mn = 1.03) and 4-armed poly(ethylene glycol) amine (Tetra-PEG-NH2, Mw = 20 kDa, Mw/Mn = 1.03) were purchased from SINOPEG, China. Agar was purchased from ENERGY CHEMICAL. Ferric acetylacetonate (Fe(acac)3), 1,2-hexadecanediol, oleylamine, oleic acid, and polyol medium triethylene glycol were purchased from J&K Scientific Ltd. All chemicals were used as received. All other chemicals were of analytical grade.
6
Nanoparticle Formulation for Drug Delivery
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Iron tri(acetyl acetonate), oleylamine, and oleic acid were purchased from J&K Chemical Ltd. (Beijing, China). DSPE-PEG 2000 was purchased from Advanced Viecle Technology Co., Ltd. (Shanghai, China). Doxorubicin hydrochloride (DOX.HCl) was purchased from Meilune Biological Technology Co., Ltd. (Dalian, China). MTT was purchased from Sigma-Aldrich Co. (Shanghai, China). DMEM and FBS were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Trypsin-EDTA (0.25%), streptomycin, and penicillin were purchased from Thermo Fisher Scientific.
7
Synthesis of Cs-oleate Precursor
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Chemicals：Cs2CO3 (99.9%) was purchased from J&K. Oleic acid (90%, OA) and octadecene (90%, ODE) were purchased from Alfa Aesar. PbBr2 (99.0%) and oleylamine (80-90%, OLA)
were purchased from Aladdin. Toluene (99.5%) was purchased from Beijing Chemical Factory.
Preparation of Cs-oleate: Cs2CO3 (0.8 g), OA (2.5 mL) and ODE (30 mL) were loaded into a 100 mL 3-neck flask and dried under vacuum for 1 h at 120 ºC. The reaction solution was then kept at 150 ºC under N2 until it became clear.
were purchased from Aladdin. Toluene (99.5%) was purchased from Beijing Chemical Factory.
Preparation of Cs-oleate: Cs2CO3 (0.8 g), OA (2.5 mL) and ODE (30 mL) were loaded into a 100 mL 3-neck flask and dried under vacuum for 1 h at 120 ºC. The reaction solution was then kept at 150 ºC under N2 until it became clear.
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